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InvisiblefahtsterM
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Re: growing clean prints in a cleanbag. [Re: fastfred]
    #7816064 - 12/31/07 06:30 AM (16 years, 1 month ago)

are you talking about micropore tape?? so you think that micropore tape is a better filter than three inches of stuffed Pressure cooked polyfil? lol do you print in front of a flowhood? how does that work? also, and I'm not sure how much clearer I can make this: the main idea is to flood caps of hard to get spores like PE.

keep the site feud BS out of my thread please.. thank you.

fahtster


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Invisiblefastfred
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Re: growing clean prints in a cleanbag. [Re: fahtster]
    #7817237 - 12/31/07 02:08 PM (16 years, 1 month ago)

> are you talking about micropore tape??

No. You can get a filter, called a micropore filter, that is useful for filtering air, or liquids as in filter sterilization. It has a membrane in it that only allows particles of a certain size or less through. Very handy to have around the lab. They cost around $5 each and usually have a leur lock for attachment to a syringe or tubing.

> lol do you print in front of a flowhood? how does that work?

Set up the caps to print in your flowhood and then cover them with some sort of container. Pretty simple and effective. I never used laminar flow hoods anyways, just top down reverse fume hood style ones, so it was easy to set up caps to print then turn off the blower and shut the front.

Perhaps my post didn't come across as complimentary as I meant it. Congrats on perfecting an interesting technique and for creating a detailed writeup. It is a useful technique to know.

> keep the site feud BS out of my thread please.. thank you.

I'm not trying to stir up any feud, just pointing out that mycotopia can't be taken seriously for any sort of research. Hippie makes no real attempt at impartiality and has banned several important members just for disagreeing with him and proving him wrong.

With a moderator who chooses sides and allows little or no criticism or even posting of facts and references you have no peer review and therefore are not a reliable source for scientific information. Another point is that the shroomery is much larger and has a forum for these types of things.

Mycotopia is very valuable as a backup site in case the shroomery were to dissolve. It is good to have multiple sites on the same topic for redundancy and competition reasons.

However posting to mycotopia vs the shroomery is comparable to a letter to the editor printed in your school newspaper vs. being published in Nature IMHO.

Sorry to stray from the topic, I just wanted to explain my earlier statement since it was taken as trying to feed a feud. Just wanted to clarify that so nobody else jumps in and we can continue on topic.

One question that came to mind...
As for flooding the caps... How can you be sure that you're recovering spores rather than just cloning tiny bits of tissue that might come off in the wash?


-FF


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OfflineRogerRabbitM
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Re: growing clean prints in a cleanbag. [Re: fastfred]
    #7817322 - 12/31/07 02:35 PM (16 years, 1 month ago)

Quote:

you have no peer review



But, isn't posting the procedure here asking for peer review?  I don't see anything wrong with the procedure itself.  It should work well. 

I think all sides need to not further any inter-site feuds.  They make both places look bad. :wink:
RR


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InvisiblefahtsterM
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Re: growing clean prints in a cleanbag. [Re: fastfred]
    #7817385 - 12/31/07 02:50 PM (16 years, 1 month ago)

well aren't you just a bundle of joy. lol  reading that post is like waking up christmas morning at the easter bunnies house.

Quote:

With a moderator who chooses sides and allows little or no criticism or even posting of facts and references you have no peer review and therefore are not a reliable source for scientific information. Another point is that the shroomery is much larger and has a forum for these types of things.





lol for someone who gives compliments, you're not very good at it.  I allow criticism, just not blunt disregard based on my title.  If I were choosing sides, you wouldn't even be reading this thread, now would you?  but, thats fine.  My comments were directed at the use of micropore tape... my bad. 

Quote:

One question that came to mind...
As for flooding the caps... How can you be sure that you're recovering spores rather than just cloning tiny bits of tissue that might come off in the wash?





it's definitely something that I've thought of, but for now, it's still a work in progress and results are still out.  but maybe I shouldn't call it work since I have no scientific value. :wink:

....ignored

fahtster


Edited by fahtster (12/31/07 03:23 PM)


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Invisibledumbfounded1600
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Re: growing clean prints in a cleanbag. [Re: the_chosen_one]
    #7924183 - 01/24/08 01:08 PM (16 years, 8 days ago)

There is no such thing as a sterile print...


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OfflineRogerRabbitM
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Re: growing clean prints in a cleanbag. [Re: dumbfounded1600]
    #7924200 - 01/24/08 01:12 PM (16 years, 8 days ago)

Sure there is. It just won't grow anything.
RR


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Invisibledumbfounded1600
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Re: growing clean prints in a cleanbag. [Re: RogerRabbit]
    #7924213 - 01/24/08 01:16 PM (16 years, 8 days ago)

:eek::eek::eek::eek::eek::eek:

*nods*


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InvisiblefahtsterM
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Re: growing clean prints in a cleanbag. [Re: dumbfounded1600]
    #7924445 - 01/24/08 02:32 PM (16 years, 8 days ago)

sorry, I have a monster headache.. I meant sterile as in clean.. I should probably have used better wording since it has a meaning so closely related in this field. I'll update this soon.. next couple days.. really busy atm.

fahtster


Edited by fahtster (01/24/08 03:18 PM)


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InvisiblefahtsterM
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Re: growing clean prints in a cleanbag. [Re: fahtster]
    #8083982 - 02/28/08 10:29 PM (15 years, 10 months ago)

alright, so it took me a little bit longer than a couple days.. more like a month to reply, BUT I did so on purpose... I didn't get normal fruits with the PE like I did with the TEX... I should have known better.. I got these "ball" fruits which I've seen with PE before.. not sure if it's not enough O2 or less etc.. or whatever, but I did try to flood the caps of a non bagged PE specimen and it didn't work very well.. so "hands to the sky" about the PE cap flooding.. not workin.. BUT I did let these go an extended period of time to see if mold would take over.. these pics were taken just a few moments ago..

this is before I broke the bag.. you can see the "ball" fruits in the container...


no mold to be seen.  Then I opened the bag and quickly took a big whiff.. and no abnormal smells were smelled to indicate bacteria.. just the earthiness of the myc..



But then I split open one of the decomposing fruits to see what was inside...



This black oily substance was inside.. I don't know if it's bacteria decomposition or if it self-decomposition, but it smelled earthy/oily as well.

This one was kinda of a nonperfect test as the inside container was sealed, this led to the container itself being malformed in the PC due to heating and cooling.. here's a pic of the bottom of the container.. you can see that it's concave.. this made the seal on the container less than desirable and I think the substrate dried out abnormally..



further tests will be done with sporelating, prolific fruit producing strains to obtain multi-use clean print jars.  just a slight setback is all.  Science excells through failure. :thumbup:

fahtster


Edited by fahtster (02/28/08 10:31 PM)


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Invisibleflavoraid
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Re: growing clean prints in a cleanbag. [Re: fahtster]
    #8084234 - 02/28/08 11:39 PM (15 years, 10 months ago)

Dare you to eat it. (the black oiling thing that is)


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imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.


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OfflineMycoAu
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Re: growing clean prints in a cleanbag. [Re: flavoraid]
    #8085279 - 02/29/08 08:53 AM (15 years, 10 months ago)

Next time, do the same grow, but use an air pump hooked to an in-line air filter and pump sterile air into the bag. Use a pin-point sized hole in the bag to allow air back out (so it doesn't over-inflate and pop). This way you have "good" air exchange and sterility is maintained without disturbing the mushrooms.


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InvisiblefahtsterM
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Re: growing clean prints in a cleanbag. [Re: MycoAu]
    #8090713 - 03/01/08 05:19 PM (15 years, 10 months ago)

well.. do you mean leave the air pump running?  I'm not sure it's not enough air or what.. I did pump air in thru the filter and suck it out twice a day like one would do with a normal FC.. I think due to imperfect fitting lid, it actually dried out too much.. that and I think I should have used a thicker substrate... I've successfully grown healthy fruits with this procedure as stated with pics in the beginning of this thread.. the TEX, but with my shitty luck the isolate turned out to be sporeless...



not a spore in sight. :frown: 

So I know this can be done, it's just a matter of getting it right and now I know that the bag is somewhat effective at keeping contams out as I kept the lid on the container and didn't air it out for a good 2 months with little to no sign of mold and seemingly no bacterial infection.  so thats good.  at least I got something out of the PE bag... that and knowing that the cap flooding is not as easy as I once thought.

fahtster


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