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Offlinegaladar


Registered: 05/09/07
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Bleach in LC
    #7655396 - 11/19/07 03:41 PM (16 years, 2 months ago)

anyone know if a tiny ammount of bleach would help keep a lc clean?
i know it dont hurt mycelium, maybe makin this ok for lc cloning or lc2lc or agar? would it be safe for spores? never thought of that

also, what ammountof bleach would be good ? maybe a 3-4 drops per 100ml?


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InvisibleBUDDHA_702
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Registered: 02/17/07
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Re: Bleach in LC [Re: galadar]
    #7655405 - 11/19/07 03:42 PM (16 years, 2 months ago)

To my knowledge Bleach will kill everything.


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Offlinebryanbzl
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Registered: 03/11/07
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Re: Bleach in LC [Re: BUDDHA_702]
    #7655432 - 11/19/07 03:46 PM (16 years, 2 months ago)

I Just finished using bleach in my LC and it worked perfectly. I would recommend it.

heres a link:
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/7654398/an/0/page/0


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Cheers,

bzl

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"From 1898 through to 1910 heroin was marketed as a non-addictive morphine substitute and cough medicine for children."

conclusion:
poor fucking children of the early 1900's.


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Offlinetotal
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Re: Bleach in LC [Re: bryanbzl]
    #7655453 - 11/19/07 03:51 PM (16 years, 2 months ago)

sigh...why add bleach? with proper sterile procedures, you shouldnt need it!


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OfflinetequilaWorm
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Re: Bleach in LC [Re: total]
    #7655538 - 11/19/07 04:09 PM (16 years, 2 months ago)

Mycelium doesn't like bleach too much, if you need to use some sort of sterilizer, I'd suggest h2o2.


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Pictures of custom timer for automated FAE coming soon... and of course, some of the musshh.


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OfflineNibin
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Re: Bleach in LC [Re: tequilaWorm]
    #7655562 - 11/19/07 04:14 PM (16 years, 2 months ago)

Quote:

tequilaWorm said:
Mycelium doesn't like bleach too much, if you need to use some sort of sterilizer, I'd suggest h2o2.




NO. H2O2 damages mycelium more than bleach. RR even dips pieces of muchroom in bleach before cloning.

But using either is pretty pointless as once you boil the jars they will decompose and cease having any effect, and neither of them will be able to fully sterilize a jar on their own (without heat) as some microorganisms can survive them.


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Newcomers guide-----> For all things shroomy


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Offlinegaladar


Registered: 05/09/07
Posts: 981
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Re: Bleach in LC [Re: Nibin]
    #7655753 - 11/19/07 04:43 PM (16 years, 2 months ago)

well every bit of sterility helps and im not saying dont boil anything, im just saying a few drops of bleach + boiling or pc can help better than boil or pc alone.

im pretty sure it would help those without a pressure cooker.


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Offlinebryanbzl
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Re: Bleach in LC [Re: galadar]
    #7656149 - 11/19/07 06:12 PM (16 years, 2 months ago)

True.. i do not have a pressure cooker and adding bleach to my LC solution proved worthwhile. i have about 240ml (CC) of solution to work with. Give it a shot, use about 5 percent in total. A little less.


--------------------
Cheers,

bzl

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------
"From 1898 through to 1910 heroin was marketed as a non-addictive morphine substitute and cough medicine for children."

conclusion:
poor fucking children of the early 1900's.


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Offlinebryanbzl
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Registered: 03/11/07
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Re: Bleach in LC [Re: bryanbzl]
    #7656165 - 11/19/07 06:14 PM (16 years, 2 months ago)

I agree with your statement Nibin... before the heat is provided i would assume that the bleach begins kicking ass to ease the load on the cooking :wink:


--------------------
Cheers,

bzl

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------
"From 1898 through to 1910 heroin was marketed as a non-addictive morphine substitute and cough medicine for children."

conclusion:
poor fucking children of the early 1900's.


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OfflineCaribou_Lou
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Registered: 10/17/07
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Re: Bleach in LC [Re: bryanbzl]
    #7656548 - 11/19/07 07:57 PM (16 years, 2 months ago)

Somebody said the left a piece of a cake in a cup of bleach and it thrived in it and there was a bunch of myc growing.


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Offlinethebrain
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Re: Bleach in LC [Re: bryanbzl]
    #7656560 - 11/19/07 07:59 PM (16 years, 2 months ago)

I have used bleach and water(1:10) and without proper ventilation, the vapors can bruise your mycelium. Does bruising use up nutrients to repair itself? If so, you are looking at this in possibly the wrong direction.


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Offlinegaladar


Registered: 05/09/07
Posts: 981
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Re: Bleach in LC [Re: thebrain]
    #7656653 - 11/19/07 08:18 PM (16 years, 2 months ago)

thats most likely caused by water droplets spashing the cake.


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OfflineFraggin
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Re: Bleach in LC [Re: galadar]
    #7656757 - 11/19/07 08:50 PM (16 years, 2 months ago)

Bleach is an oxidizing agent that serves a purpose similar to hydrogen peroxide. Some bleaches do contain hydrogen peroxide. Peroxide was developed as an alternative to chlorine bleach, even thought Hydrogen Peroxide in it's undiluted form is stronger than chlorine, chlorine dioxide, and potassium permanganate.
I would expect diluted hydrogen peroxide and diluted chlorine bleach to work very similarly in this hobby.
I would, however, stick to the proven method of utilizing H2O2.


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Offlinegaladar


Registered: 05/09/07
Posts: 981
Loc: in a monotub!
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Re: Bleach in LC [Re: Fraggin]
    #7657495 - 11/20/07 01:28 AM (16 years, 2 months ago)

how about having a syringe of bleach and let a few cc's of bleach in and then some cc's of spore solution?

as a precaution if boiling didnt kill all contams.


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Offlineveda_sticks
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Re: Bleach in LC [Re: galadar]
    #7657805 - 11/20/07 04:50 AM (16 years, 2 months ago)

There is already another discusion on the effects of bleach on pinset, although this may be slightly of topic it is still inline with the discusion of bleach.

Maybe u should take this here rather than create another topic.

If u are sterilising things, then there is no need to use additives.

H202 is not really gonna kill anything, and it will break down into 02 and h20 in a short time anyway. Its only really helpful in the fight agains cobweb

Bleach is harder on spores but it still doesnt really kill moulds.

For LC's u can make them without a pressure cooker.


--------------------

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