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Chumley1
Missing Link

Registered: 07/29/06
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the syringe/print make-up
#7653771 - 11/19/07 07:51 AM (16 years, 2 months ago) |
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My plan is to put a qt of well water inside my PC when I cook my WBS, along with the syringe, ect. My question is, how much of a print to how much water? I read till my eyes were bleeding and can't nail this one down. Chumley
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grandesign
Mushroom Madness



Registered: 08/13/07
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Re: the syringe/print make-up [Re: Chumley1]
#7653779 - 11/19/07 07:56 AM (16 years, 2 months ago) |
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what do you wanna do make a syringe ?
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impeachme2
AmateurMycologist



Registered: 05/10/07
Posts: 646
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Re: the syringe/print make-up [Re: Chumley1]
#7653780 - 11/19/07 07:57 AM (16 years, 2 months ago) |
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I'm not sure what you're asking.
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veda_sticks
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Re: the syringe/print make-up [Re: impeachme2]
#7653837 - 11/19/07 08:31 AM (16 years, 2 months ago) |
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I think he is asking how much of a spore print should be use for his syringe.
When you sterilie your syringe, it should be have water in it, so you have a 10cc syringe with just over 10cc of water in it, thats how much water you need.
A small scraping from the print will be plenty for a 10ml syringe, an eight of the print i would think be plenty.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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grandesign
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Re: the syringe/print make-up [Re: veda_sticks]
#7653844 - 11/19/07 08:32 AM (16 years, 2 months ago) |
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i wouldn mess about making multi spore syringes when you can put the spores straight into lc !
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veda_sticks
Cultivator




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Re: the syringe/print make-up [Re: grandesign]
#7653846 - 11/19/07 08:34 AM (16 years, 2 months ago) |
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tru, that reminds me, i;ve got a b+ print dying to be used
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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grandesign
Mushroom Madness



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Re: the syringe/print make-up [Re: veda_sticks]
#7653856 - 11/19/07 08:38 AM (16 years, 2 months ago) |
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i think we have killed his thread lol scrape that print into some lc is it one you have done or bought i will prob get prints in future for new strains !
-------------------- who are you ? who am i ? This shit aint real !!!
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Slimz
.-~*´`*·~-experience-~*´`*·~-.




Registered: 10/03/07
Posts: 3,588
Loc: Maryland
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Re: the syringe/print make-up [Re: grandesign]
#7653864 - 11/19/07 08:41 AM (16 years, 2 months ago) |
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i've got a B+, R44, and half an Oak Ridge just waiting to be used
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Ogla



Registered: 02/16/04
Posts: 11,314
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Re: the syringe/print make-up [Re: Slimz]
#7653879 - 11/19/07 08:47 AM (16 years, 2 months ago) |
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I've always been able to make many syringes even out of the smallest print. Ive done it before making a dark syringe, then sterilizing water in a tupperware and sucking more water back in the make the syringe go further. You sould scrape enough spores so you can see black lumps in the bottom of shot glass before exspelling the water. Thats just me thou. depends on how big and dark your print is.
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grandesign
Mushroom Madness



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Re: the syringe/print make-up [Re: Ogla]
#7653886 - 11/19/07 08:49 AM (16 years, 2 months ago) |
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yeah thats cool but im saying why make a syringe when you can make enough lc to kill a dog lol
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Ogla



Registered: 02/16/04
Posts: 11,314
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Re: the syringe/print make-up [Re: grandesign]
#7653922 - 11/19/07 09:00 AM (16 years, 2 months ago) |
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yeah, i do both. That way you can make so much spawn, you don't know what to do with it..
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grandesign
Mushroom Madness



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Re: the syringe/print make-up [Re: Ogla]
#7653935 - 11/19/07 09:04 AM (16 years, 2 months ago) |
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lol thats allways nice though
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Chumley1
Missing Link

Registered: 07/29/06
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Re: the syringe/print make-up [Re: grandesign]
#7653999 - 11/19/07 09:36 AM (16 years, 2 months ago) |
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Sorry I wasn't clear enough. I want to make enough solution to inoculate 6 qt of WBS. I only have 1 syringe and 1 print.
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grandesign
Mushroom Madness



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Re: the syringe/print make-up [Re: Chumley1]
#7654042 - 11/19/07 09:52 AM (16 years, 2 months ago) |
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well you got two options you can make a syringe or a lc both have a chance of contamination depending on your sterility do a search and compare the teks if you make lc you have an emmence amount of mycelium to ennoc a load of jars and you also have the added bonus of the spores having already germinated so the speed of mycelium growth will be that much faster to beat those nasty (chance of) contams but do the searches and decide for yourself
-------------------- who are you ? who am i ? This shit aint real !!!
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Chumley1
Missing Link

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Re: the syringe/print make-up [Re: grandesign]
#7654050 - 11/19/07 09:53 AM (16 years, 2 months ago) |
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Yes, I want to make enough spoor solution to inoculate 6 qt of WBS. I got 1 print and 1 syringe, 20ml. By the way, what does lc stand for. Is there a page anywhere that tells old farts what all the abrevations stand for?
Thanks
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grandesign
Mushroom Madness



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Re: the syringe/print make-up [Re: Chumley1]
#7654066 - 11/19/07 10:01 AM (16 years, 2 months ago) |
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sorry liquid culture (lc) do a search for the post acronyms that will tell you all the abbreviations ive never made a syringe cos liquid culture makes sooo much more myc from which you can ennoculate other jars of seed or whatever and its started growing already so gives you a head start on contams but if its a spore syringe you really want better ask someone else
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Chumley1
Missing Link

Registered: 07/29/06
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Re: the syringe/print make-up [Re: grandesign]
#7654253 - 11/19/07 11:22 AM (16 years, 2 months ago) |
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I can't do the lc at present because I got nothing to clone. I am really turned on by the LC TEK and can't wait to try it. thanks for the mention.
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grandesign
Mushroom Madness



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Re: the syringe/print make-up [Re: Chumley1]
#7654387 - 11/19/07 11:54 AM (16 years, 2 months ago) |
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you dont have to clone you can use spores cloning lc is different from spore lc
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veda_sticks
Cultivator




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Re: the syringe/print make-up [Re: grandesign]
#7655028 - 11/19/07 02:34 PM (16 years, 2 months ago) |
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Liquid cultures are great, but your sterile procedures must be down good, as its harder to spot contams in LC, but apart from that once u have a nice pint of healthy myc water, u start another set of jars up using that "master" LC to make working cultures, which once ready you use to inoc your substrate jars.
Once u get this procedure down, for the personal mycologist (one who grows a few shrooms for personal use and occasional hand out) having a master LC can last a long time.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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Nibin
Getting there



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Re: the syringe/print make-up [Re: veda_sticks]
#7655269 - 11/19/07 03:12 PM (16 years, 2 months ago) |
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Making a spore syringe can be a very good way of actually making a LC.
Instead of messing about with a glove box and opening the lids of the jars and so, to scrape the spores in, he can make the syringe and use that to make the LC or just inoculate straight.
So it costs you a couple of bucks for a syringe (that you can recicle) but it saves you a lot of hassle.
Also, if you have access to enough spores, innoculating with syringes is basically the same as with a LC. Unless the LC comes from a clone they will both be multispore, it is just that the LC will start to colonize faster (but you will have inoculated with spores a week beforehand)
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