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Offlinemad genius
Stranger


Registered: 11/10/07
Posts: 167
Last seen: 4 years, 11 months
I MADE THE HOLES TO SMALL!!
    #7653390 - 11/19/07 02:18 AM (16 years, 2 months ago)

Ok so today i attempted inoculating my first pressure cooked 250ml jars full of BRF goodness. So i get my jars out of the pressure cooker, flame my spore syringe and try to stick the syringe into the holes on top of the jar, only to realize that i made the DAMNED HOLES TO SMALL!! So I lysol'd my bathroom to death, cleaned a nail with alcohol and went to work. I opened the jars one by one and covered them with tin foil right away. I then proceeded to make the holes bigger with the disinfected nail and re-cover the jars putting the lids on and then the foil on the lids and putting the jars back in my glovebox. After that I inoculated inside the glovebox. The jars are now in the incubator. Am I screwed?


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Offlinegaladar


Registered: 05/09/07
Posts: 981
Loc: in a monotub!
Last seen: 2 years, 2 months
Re: I MADE THE HOLES TO SMALL!! [Re: mad genius]
    #7653394 - 11/19/07 02:20 AM (16 years, 2 months ago)

i wouldve started over again as brf and verm are dirt cheap and i hate waiting on a jar only to see it contam.
buuuuut i wish you luck and hope that it worked.


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Offlinegrandesign
Mushroom Madness
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Registered: 08/13/07
Posts: 488
Loc: Flag
Last seen: 1 year, 2 months
Re: I MADE THE HOLES TO SMALL!! [Re: mad genius]
    #7653401 - 11/19/07 02:25 AM (16 years, 2 months ago)

its hard to tell i wouldn worrie too much your sterility sounds ok ive had many holes close up on me i think its the heat from the pc causes the metal to expand slightly ive never had any problems yet so you should be fine the only thing i would say is not to work in the bathroom its just a thing i wouldn do i think of the bacteria that could be living in the cleanest of bathrooms but thats my opinion GD


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who are you ? who am i ? This shit aint real !!!


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OfflineTurntableJunky
Ethno Grower
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Registered: 04/26/07
Posts: 4,742
Loc: Sydney
Last seen: 15 years, 9 months
Re: I MADE THE HOLES TO SMALL!! [Re: mad genius]
    #7653445 - 11/19/07 03:14 AM (16 years, 2 months ago)

Did you use a dry verm barrier?


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Offlinemad genius
Stranger


Registered: 11/10/07
Posts: 167
Last seen: 4 years, 11 months
Re: I MADE THE HOLES TO SMALL!! [Re: TurntableJunky]
    #7653916 - 11/19/07 08:58 AM (16 years, 2 months ago)

Of course.


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OfflineRogerRabbitM
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: I MADE THE HOLES TO SMALL!! [Re: mad genius]
    #7654017 - 11/19/07 09:41 AM (16 years, 2 months ago)

If you used a dry verm barrier, you can remove the lid anytime you need to. It's only there to restrict evaporation of the moisture from the substrate.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Offlineveda_sticks
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Posts: 14,191
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Re: I MADE THE HOLES TO SMALL!! [Re: RogerRabbit]
    #7654022 - 11/19/07 09:43 AM (16 years, 2 months ago)

And to prevent contams reaching the substrate?


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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OfflineRogerRabbitM
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Re: I MADE THE HOLES TO SMALL!! [Re: veda_sticks]
    #7654044 - 11/19/07 09:52 AM (16 years, 2 months ago)

Quote:

veda_sticks said:
And to prevent contams reaching the substrate?



No. That's the job of the verm filter.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Offlinemad genius
Stranger


Registered: 11/10/07
Posts: 167
Last seen: 4 years, 11 months
Re: I MADE THE HOLES TO SMALL!! [Re: RogerRabbit]
    #7654424 - 11/19/07 12:00 PM (16 years, 2 months ago)

I appreciate all your feedback guys. This site is the best.


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Offlineveda_sticks
Cultivator
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Folding@home Statistics
Registered: 07/29/07
Posts: 14,191
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Re: I MADE THE HOLES TO SMALL!! [Re: mad genius]
    #7654906 - 11/19/07 02:07 PM (16 years, 2 months ago)

well as they say where i come from

"Ya nuggett"


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


Extras: Filter Print Post Top
Offlineveda_sticks
Cultivator
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Folding@home Statistics
Registered: 07/29/07
Posts: 14,191
Loc: UK Flag
Last seen: 4 years, 25 days
Re: I MADE THE HOLES TO SMALL!! [Re: veda_sticks]
    #7654976 - 11/19/07 02:24 PM (16 years, 2 months ago)

ok im confused again,


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


Extras: Filter Print Post Top
Offlinemad genius
Stranger


Registered: 11/10/07
Posts: 167
Last seen: 4 years, 11 months
Re: I MADE THE HOLES TO SMALL!! [Re: veda_sticks]
    #7656610 - 11/19/07 08:09 PM (16 years, 2 months ago)

???


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Invisibletakk
Stranger
Male

Registered: 02/22/06
Posts: 736
Re: I MADE THE HOLES TO SMALL!! [Re: mad genius]
    #7656751 - 11/19/07 08:49 PM (16 years, 2 months ago)

*smacks forhead* :laugh:


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InvisibleMojo
Stranger

Registered: 07/12/07
Posts: 1,676
Re: I MADE THE HOLES TO SMALL!! [Re: veda_sticks]
    #7656899 - 11/19/07 09:38 PM (16 years, 2 months ago)

Quote:

veda_sticks said:
ok im confused again,




I think what RR means is that the lid is only there to restrict evaporation from the substrate so it doesn’t matter if you remove it temporarily.


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Offlineveda_sticks
Cultivator
Male User Gallery


Folding@home Statistics
Registered: 07/29/07
Posts: 14,191
Loc: UK Flag
Last seen: 4 years, 25 days
Re: I MADE THE HOLES TO SMALL!! [Re: Mojo]
    #7657855 - 11/20/07 05:24 AM (16 years, 2 months ago)

ah, i understand i misread it, i thought he was talking about the dry verm layer stoping moisture escaping, and i got all con fusse!


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


Extras: Filter Print Post Top
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