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Mycodood



Registered: 05/22/07
Posts: 277
Loc: USA
Last seen: 2 years, 11 months
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Cloning idea - Would like suggestions
#7638935 - 11/15/07 10:25 AM (16 years, 2 months ago) |
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I don't have a glovebox nor do I want one due to lack of space in my home.
I have tried a couple of open air clone transfers to LC and have had miserable failures (FYI - I did not soak the tissue in H202 prior)
So an idea I had is this (may not be a new idea).
- Make up an LC of choice and set aside.
- Take a sterilized syringe and drop a few pices of tissue int the
syringe and replace the plunger.
- Draw up some H202 enough to clean tissue and bang it around a bit
to break up the tissue so some pieces will transfer to the LC.
- Expel enough of the H202 so I have the minimal amount when it comes
time to flame sterilize and inoc the LC
Does anyone see any problems with this? Any suggestions? What is the minimum amount of H202 that I should inject into say 300 ML of LC?
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lipa


Registered: 07/24/07
Posts: 2,684
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Re: Cloning idea - Would like suggestions [Re: Mycodood]
#7639103 - 11/15/07 10:56 AM (16 years, 2 months ago) |
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I give it a 60-70 % success rate. I could be wrong though. I would change your process. Take the fungus and wipe it down with a 10 % bleach solution. Tear it open and grab a piece of tissue and quickly put it in the syringe. Forget about the peroxide. Suck up some sterile water, shake the hell out of it ,and inoculate.
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CantiSama


Registered: 10/21/04
Posts: 794
Last seen: 4 months, 10 days
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Re: Cloning idea - Would like suggestions [Re: lipa]
#7639170 - 11/15/07 11:08 AM (16 years, 2 months ago) |
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Search on the forum for cloning by taking a "plug" with a syringe. it works really well and requires no flowhood/still air box, etc. at least i didn't use one
--------------------
Tiger got to hunt, bird got to fly; Man got to sit and wonder 'why, why, why?' Tiger got to sleep, bird got to land; Man got to tell himself he understand.
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Slimz
.-~*´`*·~-experience-~*´`*·~-.




Registered: 10/03/07
Posts: 3,588
Loc: Maryland
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Re: Cloning idea - Would like suggestions [Re: CantiSama]
#7639218 - 11/15/07 11:16 AM (16 years, 2 months ago) |
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is a fresh bottle of distilled water from your grocer sterile?
-------------------- Lazy Drywall Tek (no powdery mess) This series will blow your mind and confirm what you already know to be true. The Pharmacratic Inquisition Best Thread Ever ! ! !
me if you have questions about lasers Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles. FeelFamily resident tech guru
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CantiSama


Registered: 10/21/04
Posts: 794
Last seen: 4 months, 10 days
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Re: Cloning idea - Would like suggestions [Re: Slimz]
#7639233 - 11/15/07 11:19 AM (16 years, 2 months ago) |
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probably but i wouldn't risk it. get a pot of water boiling and take a pull of water, give it a few seconds, expel and repeat a few times. tap water is fine, too.
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Tiger got to hunt, bird got to fly; Man got to sit and wonder 'why, why, why?' Tiger got to sleep, bird got to land; Man got to tell himself he understand.
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Mycodood



Registered: 05/22/07
Posts: 277
Loc: USA
Last seen: 2 years, 11 months
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Re: Cloning idea - Would like suggestions [Re: CantiSama]
#7639688 - 11/15/07 01:04 PM (16 years, 2 months ago) |
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Quote:
CantiSama said: Search on the forum for cloning by taking a "plug" with a syringe. it works really well and requires no flowhood/still air box, etc. at least i didn't use one
I tried this and could never get a sample to fill the needle.
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CantiSama


Registered: 10/21/04
Posts: 794
Last seen: 4 months, 10 days
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Re: Cloning idea - Would like suggestions [Re: Mycodood]
#7639951 - 11/15/07 02:09 PM (16 years, 2 months ago) |
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i had the same problem at first, i think i went vertically against the stem and got a little chunk. doesn't take much. i got a few syringes from sporeworks for halloween and they are huge so that should make it easier next time.
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Tiger got to hunt, bird got to fly; Man got to sit and wonder 'why, why, why?' Tiger got to sleep, bird got to land; Man got to tell himself he understand.
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: Cloning idea - Would like suggestions [Re: CantiSama]
#7639973 - 11/15/07 02:15 PM (16 years, 2 months ago) |
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You probably have a dozen gloveboxes hanging around your place right now. All you need is a plastic trash bag. It helps if it's clear or white. Simply stick your hands and product inside and do your work. Open air has a much lower success rate. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Mycodood



Registered: 05/22/07
Posts: 277
Loc: USA
Last seen: 2 years, 11 months
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Re: Cloning idea - Would like suggestions [Re: Mycodood]
#7640076 - 11/15/07 02:38 PM (16 years, 2 months ago) |
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So is bleach really better at cleaning the specimen?
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Mycodood



Registered: 05/22/07
Posts: 277
Loc: USA
Last seen: 2 years, 11 months
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Re: Cloning idea - Would like suggestions [Re: RogerRabbit]
#7640088 - 11/15/07 02:40 PM (16 years, 2 months ago) |
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Quote:
RogerRabbit said: You probably have a dozen gloveboxes hanging around your place right now. All you need is a plastic trash bag. It helps if it's clear or white. Simply stick your hands and product inside and do your work. Open air has a much lower success rate. RR
Thanks RR. I was thinking about doing this as well. So should I just Oust the inside of the bag, clean everything down place it in the bag and do my work?
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lipa


Registered: 07/24/07
Posts: 2,684
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Re: Cloning idea - Would like suggestions [Re: Mycodood]
#7643318 - 11/16/07 08:43 AM (16 years, 2 months ago) |
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Quote:
Mycodood said: So is bleach really better at cleaning the specimen?
My own personal opinion. YES!
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 25 days
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Re: Cloning idea - Would like suggestions [Re: lipa]
#7643332 - 11/16/07 08:50 AM (16 years, 2 months ago) |
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Nothing fancy about a glovebox,
A plastic container with 2 circles cut out, as long as u can see inside then there you go, glovebox.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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Mycodood



Registered: 05/22/07
Posts: 277
Loc: USA
Last seen: 2 years, 11 months
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Re: Cloning idea - Would like suggestions [Re: veda_sticks]
#7658198 - 11/20/07 09:24 AM (16 years, 2 months ago) |
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Quote:
veda_sticks said: Nothing fancy about a glovebox,
A plastic container with 2 circles cut out, as long as u can see inside then there you go, glovebox.
Umm...thanks but that wasn't my original question.
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grandesign
Mushroom Madness



Registered: 08/13/07
Posts: 488
Loc:
Last seen: 1 year, 2 months
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Re: Cloning idea - Would like suggestions [Re: Mycodood]
#7658231 - 11/20/07 09:39 AM (16 years, 2 months ago) |
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i once heard rr tell someone to use a trash bag preferable a clear one to act as a still air enviroment you could even make a type of frame for it too providing the room is clean and you sterilise every thing you should be ok when i done my lc clone i jabbed the stem out side the glove box then moved into the glove box to squirt the tissue peice out of the syringe so i may as well not have used one also make sure there is no moving air except what you are causing by moving you should be fine
-------------------- who are you ? who am i ? This shit aint real !!!
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Nibin
Getting there



Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
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Re: Cloning idea - Would like suggestions [Re: grandesign]
#7658861 - 11/20/07 12:15 PM (16 years, 2 months ago) |
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-------------------- Newcomers guide-----> For all things shroomy
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Mycodood



Registered: 05/22/07
Posts: 277
Loc: USA
Last seen: 2 years, 11 months
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Re: Cloning idea - Would like suggestions [Re: Nibin]
#7662225 - 11/21/07 10:24 AM (16 years, 2 months ago) |
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Edited by Mycodood (11/21/07 10:24 AM)
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