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Offlinelittlebigshoe
The dude

Registered: 10/09/07
Posts: 12
Loc: south dakota
Last seen: 15 years, 10 months
myco bags.... 3 weeks and sign jof myc.
    #7635521 - 11/14/07 02:49 PM (16 years, 2 months ago)

I inoculated my 3lb bags of rye grain with 5cc's of alacabenzi strain, i first put the needle over an alcohol lamp until it was red, i then cooled it down using an alcohol soaked cotton pad. and i wiped down the enviorment as well as my hands and the bags before i injected the spore solution. well that was three weeks ago and nothing is happening not even contams. i have had it in a box at a constant 81 degrees. i dont know whats wrong. should i just throw them out and start over or just wait longer. the only thing i can think that went wrong is maybe the seringe wasnt cooled down all the way and i killed the spores


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InvisibleSlimz
.-~*´`*·~-experience-~*´`*·~-.
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Folding@home Statistics
Registered: 10/03/07
Posts: 3,588
Loc: Maryland
Re: myco bags.... 3 weeks and sign jof myc. [Re: littlebigshoe]
    #7635534 - 11/14/07 02:53 PM (16 years, 2 months ago)

shake it up and give it a week.. then decide


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Offlinelittlebigshoe
The dude

Registered: 10/09/07
Posts: 12
Loc: south dakota
Last seen: 15 years, 10 months
Re: myco bags.... 3 weeks and sign jof myc. [Re: Slimz]
    #7635563 - 11/14/07 03:00 PM (16 years, 2 months ago)

i tried that a week ago
any ideas


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SO THERE I WAS.....HOLY %#@&...THERE I AM!


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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: myco bags.... 3 weeks and sign jof myc. [Re: littlebigshoe]
    #7635591 - 11/14/07 03:05 PM (16 years, 2 months ago)





here are my 2 bags after two weeks... thats odd man. 3 weeks and NO colonization. mine are spawn bags though.


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InvisibleOmnicracker
Crusted Trolltivator

Registered: 09/18/05
Posts: 1,421
Loc: Wal-Merica
Trusted Cultivator
Re: myco bags.... 3 weeks and sign jof myc. [Re: littlebigshoe]
    #7635595 - 11/14/07 03:06 PM (16 years, 2 months ago)

your grains might be too dry. or you got a bad syringe. but ive had that happen a number of times with rye when i didnt hydrate it enough


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Offlinelittlebigshoe
The dude

Registered: 10/09/07
Posts: 12
Loc: south dakota
Last seen: 15 years, 10 months
Re: myco bags.... 3 weeks and sign jof myc. [Re: Omnicracker]
    #7635672 - 11/14/07 03:19 PM (16 years, 2 months ago)

thanks for the info man. and i'm not sure if what i have are myco bags exactly... there is a self healing injection port and a filter patch. i got them from a very reputable company.
anyway... back to the drawing board i guess... im trying quart jars this time so i know the moisture level is right. any other thoughts are welcome ... thanks guys


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Offlineemilbus
Drunk
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Registered: 10/01/06
Posts: 1,113
Loc: Texas
Last seen: 16 years, 1 month
Re: myco bags.... 3 weeks and sign jof myc. [Re: littlebigshoe]
    #7637795 - 11/14/07 11:26 PM (16 years, 2 months ago)

im having some what of the same problem. mine has only been about a week and half, maybe two weeks. the temp has been between 71-78. i have one of those dig temp gauges right next to it. im thinking about shaking that bitch up but i dont want to fuck shit up.


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OfflineQDP843
Entrepreneur


Registered: 03/05/07
Posts: 295
Loc: SOUTHERN
Last seen: 15 years, 3 months
Re: myco bags.... 3 weeks and sign jof myc. [Re: emilbus]
    #7637811 - 11/14/07 11:31 PM (16 years, 2 months ago)

hey dont know but 5 ccs of solution might be too much when i first started i put 6 ccs into a 2 pound bag and mine started to grow but stop and never worked.


i think it should of been like 2-3?

im not sure just trying to help.


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Offlineemilbus
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Registered: 10/01/06
Posts: 1,113
Loc: Texas
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Re: myco bags.... 3 weeks and sign jof myc. [Re: QDP843]
    #7637918 - 11/15/07 12:04 AM (16 years, 2 months ago)

i put around 2-3cc's


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You look down at me and you see a fool
You look up at me and you see a god
You look straight at me and you see yourself


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Offlinelittlebigshoe
The dude

Registered: 10/09/07
Posts: 12
Loc: south dakota
Last seen: 15 years, 10 months
Re: myco bags.... 3 weeks and sign jof myc. [Re: emilbus]
    #7638106 - 11/15/07 01:18 AM (16 years, 2 months ago)

thanks guys for all your help. hopefully next attempt will be successful.


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Offlineyepyepyep
Stranger
Registered: 09/05/07
Posts: 147
Last seen: 26 days, 8 hours
Re: myco bags.... 3 weeks and sign jof myc. [Re: QDP843]
    #7638330 - 11/15/07 04:09 AM (16 years, 2 months ago)

Quote:

QDP843 said:
hey dont know but 5 ccs of solution might be too much when i first started i put 6 ccs into a 2 pound bag and mine started to grow but stop and never worked.


i think it should of been like 2-3?

im not sure just trying to help.




You can get away with less but that is not a particularly extravagant amount of solution and it certainly wouldn't factor into why he has had no growth.


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Offlineyepyepyep
Stranger
Registered: 09/05/07
Posts: 147
Last seen: 26 days, 8 hours
Re: myco bags.... 3 weeks and sign jof myc. [Re: QDP843]
    #7638332 - 11/15/07 04:14 AM (16 years, 2 months ago)

Quote:

QDP843 said:
hey dont know but 5 ccs of solution might be too much when i first started i put 6 ccs into a 2 pound bag and mine started to grow but stop and never worked.


i think it should of been like 2-3?

im not sure just trying to help.




You can get away with less but that is not a particularly extravagant amount of solution and it certainly wouldn't factor into why he has had no growth.


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