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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
shakin my grains
    #7635447 - 11/14/07 02:34 PM (16 years, 2 months ago)




i shook this jar right after inoculation and was wondering if would be a good idea to shake it again around 1/3 colonization? and then wait for it to fully colonize after that. thanks


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Offlineurayasan
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Re: shakin my grains [Re: blood4blood]
    #7635491 - 11/14/07 02:43 PM (16 years, 2 months ago)

I may be incorrect in my judgment of the image there, but to me it looks like there is fairly even growth throughout the jar. Shaking mostly serves to achieve even growth such as that, so if you think it appears even to you at this point then another shake will likely not accomplish much.


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Invisibleblood4blood
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Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: shakin my grains [Re: urayasan]
    #7635506 - 11/14/07 02:46 PM (16 years, 2 months ago)

yea thats what i was wondering. because the whole point of shaking is to get mycelium distributed throughout the entire jar so that it WILL fully colonize right?

i dont know. ill just leave it alone and see what happens i guess. thanks for the advice though urayasan...


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: shakin my grains [Re: blood4blood]
    #7635543 - 11/14/07 02:54 PM (16 years, 2 months ago)

I agree, for that particular jar, if it's like that all over...
leave it.

don't shake if you don't have to.
nice jar.


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Invisibleblood4blood
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Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: shakin my grains [Re: monstermitch]
    #7635559 - 11/14/07 02:59 PM (16 years, 2 months ago)

yea i shook this particular jar right after inoculation. ive read not to do that but i think it turned out quite well. thanks guys


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Offlineurayasan
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Re: shakin my grains [Re: blood4blood]
    #7635580 - 11/14/07 03:03 PM (16 years, 2 months ago)

I'm a bit on the fence about shaking vs. not shaking following inoculation. At times it seems to me that not shaking, and allowing compatible spores to find each other faster, results in considerably faster growth when all is said and done. But I have also had jars like yours, where they have been shaken and resulted in wonderfully even growth everywhere that fully colonized rapidly. I've also had jars shaken just the same which took absolutely forever, only having spotty growth in a few places. My only guess is that perhaps the spore concentrations somehow affect whether shaking is as effective, I don't really know.


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InvisibleOmnicracker
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Re: shakin my grains [Re: urayasan]
    #7635606 - 11/14/07 03:08 PM (16 years, 2 months ago)

Quote:

urayasan said:
I'm a bit on the fence about shaking vs. not shaking following inoculation. At times it seems to me that not shaking, and allowing compatible spores to find each other faster, results in considerably faster growth when all is said and done. But I have also had jars like yours, where they have been shaken and resulted in wonderfully even growth everywhere that fully colonized rapidly. I've also had jars shaken just the same which took absolutely forever, only having spotty growth in a few places. My only guess is that perhaps the spore concentrations somehow affect whether shaking is as effective, I don't really know.




unless of course we're talking about LCs, which should definately be shook up after inoculation. otherwise, learn to make LCs.


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Invisibleblood4blood
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Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: shakin my grains [Re: Omnicracker]
    #7635619 - 11/14/07 03:10 PM (16 years, 2 months ago)

yea this was multispore. i think im going to make a grain lc out of this though following one of agar's teks.

btw what percent colonized do you think this is?
its my first time at grains.


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Invisibleblood4blood
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Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: shakin my grains [Re: blood4blood]
    #7639689 - 11/15/07 01:04 PM (16 years, 2 months ago)






here are a couple pics from just a couple minutes ago. like i said earlier, this is my first time at grains and i couldnt find anything in the forum on what a 100% colonized jar looks like. so what do you guys think? does the entire surface have to be covered in white?


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OfflineCaribou_Lou
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Re: shakin my grains [Re: blood4blood]
    #7639694 - 11/15/07 01:05 PM (16 years, 2 months ago)

I think once it shrinks away from the sides then it's 100% or very close, but I'm not positive.


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InvisibleSlimz
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Re: shakin my grains [Re: Caribou_Lou]
    #7639722 - 11/15/07 01:12 PM (16 years, 2 months ago)

when in doubt.. wait 2 days .. if its more colonized, it wasn't 100%


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Offlinemilkman
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Re: shakin my grains [Re: Slimz]
    #7639861 - 11/15/07 01:45 PM (16 years, 2 months ago)

how many ccs did you put in it

and it looks like its gonna be a bitch to get it out unless you use a spoon or something similar i can never find the fully wide mouth jars that are a quart or bigger theyre always just a little too small around the top


Edited by milkman (11/15/07 01:47 PM)


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Invisibleblood4blood
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Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: shakin my grains [Re: milkman]
    #7639866 - 11/15/07 01:47 PM (16 years, 2 months ago)

3 cc's


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InvisibleOmnicracker
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Re: shakin my grains [Re: blood4blood]
    #7647367 - 11/17/07 11:43 AM (16 years, 2 months ago)

often when a substrate is at 100% the mycelium will become more rhizomorphic because its shifting into fruiting mode. peep your rhizomes.


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Offlinedrwatson
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Re: shakin my grains [Re: Omnicracker]
    #7647379 - 11/17/07 11:49 AM (16 years, 2 months ago)

Quote:

Omnicracker said:
  peep your rhizomes.




Is it just me, or does that sound naughty? :grin:


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OfflineBanjoMojo
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Re: shakin my grains [Re: drwatson]
    #7647431 - 11/17/07 12:08 PM (16 years, 2 months ago)

Do you plan to case or utilize a bulk method?


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