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Offlineveda_sticks
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Re: B+ and colonization [Re: thatamatt]
    #7650048 - 11/18/07 04:58 AM (16 years, 2 months ago)

Some people have not seen germination until as late as 2 weeks, so u will just have to wait, 4 days is quite early, 3 days is the minimum time that germination will happen.

BRF jars are not slow at germination, i have seen germination in my jars in as little as 3 days but generaly is day 6 or 7 before i see anything.

I have inoced 4-5 hours after sterilising and they have worked.

Its best to let your pressure cooker cool for 12 hours just to be safe.

Also remember that if the moisture content is off this can have an effect.


--------------------

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Foo Mans No Soak WBS Prep Tek


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Offlinelester
String Snapper


Registered: 05/18/07
Posts: 265
Last seen: 10 years, 3 months
Re: B+ and colonization [Re: thatamatt]
    #7652757 - 11/18/07 09:29 PM (16 years, 2 months ago)

Ok - another thing: I re-covered the innoc. holes with tape before putting them away .. does this slow colonization? Do they need some air now?


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Invisiblethatamatt
reaching for the secret
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Registered: 09/16/07
Posts: 168
Loc: Pangaea
Re: B+ and colonization [Re: lester]
    #7653198 - 11/19/07 12:20 AM (16 years, 2 months ago)

There's enough air for now. Leave the tape on until you see the mycelium and then remove. The first time I did not use tape on the holes. guess what happened?  10 out of 12 were infected with greenish mold about the same time the mycelium appeared. It least a got 2 cakes flushing, as I type this.
Good Luck:smirk:


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i cry for the moon


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Offlinelester
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Registered: 05/18/07
Posts: 265
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Re: B+ and colonization [Re: thatamatt]
    #7654556 - 11/19/07 12:29 PM (16 years, 2 months ago)

Thanks m8 - I'll keep you posted ..


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Offlinelester
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Registered: 05/18/07
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Re: B+ and colonization [Re: veda_sticks]
    #7658008 - 11/20/07 07:20 AM (16 years, 2 months ago)

Quote:

veda_sticks said:
Also remember that if the moisture content is off this can have an effect.




I can see moisture at the top of the jar as well as notices the whitening in the BRF where I inoculated, so I think it still has a chance ..


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Offlinelester
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Re: B+ and colonization [Re: lester]
    #7667758 - 11/22/07 06:25 PM (16 years, 2 months ago)

Now I see some pinking .. but really sparse .. 13 days now.

Put the jars on front of a heater to warm a bit .. still around 70 deg.

Is there a shelf life for syringes, as these weren't used for 2 months after delivery? ..


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Invisibleblackfir
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Registered: 10/18/07
Posts: 88
Re: B+ and colonization [Re: lester]
    #7668052 - 11/22/07 07:53 PM (16 years, 2 months ago)

\I would say after a few months you should be okay.I've always read to keep them in the fridge until use. I would try to incubate at 78-80 degrees. I think 70's a tad too cold. Pink might not be so good.


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Tell me, I pray thee, where the seer's house is.


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Offlinelester
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Re: B+ and colonization [Re: blackfir]
    #7670322 - 11/23/07 12:33 PM (16 years, 2 months ago)

Well maybe since I'm such a n00b, does the gradual whitening indicate colonization, or is it just moist BRF? I've seen 100% colonization on pics, but it looks more "spongy" than my jars ..


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Invisibleblackfir
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Registered: 10/18/07
Posts: 88
Re: B+ and colonization [Re: lester]
    #7670457 - 11/23/07 12:59 PM (16 years, 2 months ago)

Yes, as long as coming out of the points at which you inoculated. like this here
not my pic BTW. My friends didn't look so white and fluffy either. I think alot of that has to do with the flash. Once out of the jars they look much better. IMO anyway.


--------------------
Tell me, I pray thee, where the seer's house is.


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Offlinelester
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Registered: 05/18/07
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Re: B+ and colonization [Re: blackfir]
    #7674349 - 11/24/07 12:15 PM (16 years, 2 months ago)

Here they are after exactly 2 weeks:




dead?


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Offlinepazzy
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Registered: 08/24/07
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Re: B+ and colonization [Re: lester]
    #7674376 - 11/24/07 12:23 PM (16 years, 2 months ago)

Why did you only fill the jars half full?

You should have filled them to about a quarter inch from the top with pf mix then used dry vermiculite to fill them to the top.

They don't look dead though they are just colonizing really slow.


--------------------
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:yinyang2:

Absolutely no source checks/discussions.


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Offlinelester
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Registered: 05/18/07
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Re: B+ and colonization [Re: pazzy]
    #7674399 - 11/24/07 12:29 PM (16 years, 2 months ago)

Ya, the tek I was following had the mixtures for half pint jars, and these are bigger (had to borrow them). I looked around and it appears using bigger jars is ok.

So, maybe the colonization is slower because of the jar size?


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OfflineMarlboroMan
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Re: B+ and colonization [Re: pazzy]
    #7674401 - 11/24/07 12:30 PM (16 years, 2 months ago)

Quote:

pazzy said:
Why did you only fill the jars half full?

You should have filled them to about a quarter inch from the top with pf mix then used dry vermiculite to fill them to the top.



:thumbup:

What temperature do you have them at? :rockon:


--------------------
I'll make it to the moon if I have to crawl..


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Offlinelester
String Snapper


Registered: 05/18/07
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Re: B+ and colonization [Re: MarlboroMan]
    #7674412 - 11/24/07 12:32 PM (16 years, 2 months ago)

Had them in front of a heater set at 64 .. now moved it up to 70


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Offlinelester
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Re: B+ and colonization [Re: MarlboroMan]
    #7684870 - 11/27/07 07:54 AM (16 years, 2 months ago)

Would the jars half full stop colonization - of just slow it down?

Should I crack them and add more dry verm on the top?


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Offlinemad genius
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Registered: 11/10/07
Posts: 167
Last seen: 4 years, 11 months
Re: B+ and colonization [Re: lester]
    #7684979 - 11/27/07 08:41 AM (16 years, 2 months ago)

No do not open the jars, from what ive been told its a high risk for contams. The temperature is probably why your mycelium is movin so slow. Ideal incubation temp. is about 80F.


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OfflineBlargIAmDead
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Re: B+ and colonization [Re: mad genius]
    #7685546 - 11/27/07 11:20 AM (16 years, 2 months ago)

Room temp is fine. 70-75 is good. Yes it goes slower than higher temps but bacteria also like higher temps.


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Offlinelester
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Registered: 05/18/07
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Re: B+ and colonization [Re: BlargIAmDead]
    #7694684 - 11/29/07 12:43 PM (16 years, 2 months ago)

Still nothing .. tomorrow is 3 weeks. They've stayed about the same as above 2 pics of jars ..

Damn, do I need a contained chamber at the right temp?


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OfflineKCizzle
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Registered: 08/27/07
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Re: B+ and colonization [Re: lester]
    #7694851 - 11/29/07 01:27 PM (16 years, 2 months ago)

I would def keep them at 82-86. Bacteria likes it but just keep your area nice and clean. 60-70 was most definitely the reason for slow colonization.


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Offlinelester
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Re: B+ and colonization [Re: KCizzle]
    #7695391 - 11/29/07 03:49 PM (16 years, 2 months ago)

The advice is really helpful - thanks.

I actually had them in a thermos container (for picnics), angled sideways towards a heater which blew warm air into the container. At a couple of points, I felt the jars and they were bordering on HOT, so I pulled them out for a while. My room temp fluctuates between 68-70 depending on the time of the day.

So - should I keep them out of the container at 68-70, or in it? (putting them at less peril by keeping them away from the direct blast of the heater fan ..)

Thanks!


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