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Tightmopedman9
Stranger

Registered: 11/08/07
Posts: 15
Last seen: 14 years, 8 months
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How long can liquid culture be stored for? 1
#7631168 - 11/13/07 03:41 PM (16 years, 2 months ago) |
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Id like to use 1/4 of the liquid culture every 2-3 weeks or so. Would it stay viable for a month or two?
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Funkatron9000
Astronaut



Registered: 10/03/07
Posts: 535
Loc: Sector Zed: Region 6: Sol...
Last seen: 2 years, 2 months
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Re: How long can liquid culture be stored for? [Re: Tightmopedman9] 1
#7631326 - 11/13/07 04:21 PM (16 years, 2 months ago) |
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yea it'll be fine, toss it in the fridge and it can be stored for months.
-------------------- You cannot yet touch my daughters awesome boobage
You must fight to the death in the Breastriary of Nippopolis.
I thought Gene Wilder was cool BEFORE he was dead.
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BUDDHA_702
Master Mycologist In Training



Registered: 02/17/07
Posts: 1,296
Loc: Some Country
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Re: How long can liquid culture be stored for? [Re: Funkatron9000] 1
#7631722 - 11/13/07 05:31 PM (16 years, 2 months ago) |
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I've heard of LC's lasting over a year.
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SFsorrow
Is Born


Registered: 11/11/07
Posts: 259
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Re: How long can liquid culture be stored for? [Re: BUDDHA_702] 1
#7632351 - 11/13/07 07:41 PM (16 years, 2 months ago) |
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Thats amazing! I am gonna have to try this out.
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ozzyozzyozzy
Australian



Registered: 10/23/06
Posts: 545
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Re: How long can liquid culture be stored for? [Re: SFsorrow] 1
#7632365 - 11/13/07 07:45 PM (16 years, 2 months ago) |
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mine's been sitting in the cupboard and is over 8 months old
im going to have to knock up another one though because i didnt put in enough honey and so im running out of LC :P
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Tightmopedman9
Stranger

Registered: 11/08/07
Posts: 15
Last seen: 14 years, 8 months
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Re: How long can liquid culture be stored for? [Re: BUDDHA_702] 1
#7632647 - 11/13/07 08:58 PM (16 years, 2 months ago) |
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cool thanks
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wwwshroom
Old GulfCoast Tripinoid


Registered: 07/30/03
Posts: 36
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Re: How long can liquid culture be stored for? [Re: Tightmopedman9] 2
#20843253 - 11/15/14 10:04 PM (9 years, 2 months ago) |
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I've kept liquid cultures more than year in a dark place without refrigeration. Unless you place the jars in a sterile, enclosed container--I use a plastic storage box sprayed with disinfectant after each time it's opened--you run a high risk of contamination in a refrigerator. It's best if you have dedicated refrigerator if you feel you must keep it cold. My 11 years of growing indicates refrigeration is unnecessary.
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Myconomy
High-Wizard

Registered: 11/14/14
Posts: 19
Last seen: 9 years, 1 month
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Re: How long can liquid culture be stored for? [Re: wwwshroom] 1
#20843272 - 11/15/14 10:11 PM (9 years, 2 months ago) |
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I've had refrigerated and room temp LC's last well over a year. I'm just going through a bunch now that are well over 2 years old, and some seem to have recovered just fine.
wwwshroom is right about the refrigerator, I've had molds get in, and eat my mycelium, and you can't even see it happen. You just go back to an old LC one day, and it grows green on you.
That's using 2 RTV silicone injection ports, every so often either the lid bypasses, or the port leaks, from the expansion and contraction of the vapors in the jar as the temperature changes. If you're using your LC that fast, refrigeration is not necessary. I'd only ever recommend it for long term storage, and in a clean container. (although I just bleach bomb mine before use)
-------------------- Hydrogen is an invisible, odorless gas, that when given enough time turns into people... and mushrooms.
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 24 days
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Re: How long can liquid culture be stored for? [Re: Myconomy] 1
#20844505 - 11/16/14 08:29 AM (9 years, 2 months ago) |
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I have never had success with long term storage.
People say it does better with less nutrients, and storing in distilled water.
A way to do this is get a large 60cc syringe, suck up some myc dense LC and leave the syringe to settle, needle pointing upwards. Now when settled you hold it over a sink or outside and squirt out the top liquid level. Now get a jar full of sterile distilled water and inject this into it. You have now diluted the remaining nutrients a lot. This could be repeated once or twice more, letting the myc settle again and again inject into a new jar of distilled water, further diluting down any nutrients.
You can also start with a low nutrient broth to begin with. Workman was recommending a broth of only 0.1-0.4% sugar.
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Myconomy
High-Wizard

Registered: 11/14/14
Posts: 19
Last seen: 9 years, 1 month
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Re: How long can liquid culture be stored for? [Re: blackout] 1
#20873483 - 11/22/14 03:56 AM (9 years, 2 months ago) |
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Quote:
blackout said: I have never had success with long term storage.
People say it does better with less nutrients, and storing in distilled water...
...You can also start with a low nutrient broth to begin with. Workman was recommending a broth of only 0.1-0.4% sugar.
How long are we talking? Over a year?
The low nutrient broth seems to be solid advice though. I've tried varying the levels of sugar from 2-8% for the Turbinado LC, and it really seemed much better around the 2% range than 8%. Clearer, less sugar used, not very much difference in mycelial mass.
I haven't tried anything lower than 2% yet myself, but I will definitely be experimenting in this range soon.
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 24 days
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Re: How long can liquid culture be stored for? [Re: Myconomy] 1
#20873520 - 11/22/14 04:44 AM (9 years, 2 months ago) |
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Quote:
Myconomy said: I have never had success with long term storage.
I don't think I had an LC still viable after maybe 2months. Most kept about 15-18C
I have heard of people using weak agar, I think it was that it forced the myc to stretch out to "search" for new nutrients. I guess the LC is the same idea, you might force it to expand outwards more. A lot of my LCs were weak as I microwaved them which drove off moisture and concentrated the sugars. So I would be careful not to have too much sugar from the start.
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