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mycocurious
Mike O. Kuerias



Registered: 02/09/07
Posts: 1,265
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Sporeless Mutant From Poor Performing Isolate?
#7617113 - 11/09/07 10:33 PM (16 years, 3 months ago) |
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I grew out an isolate that turned out to be a poor performer but it netted me a sporeless mutant fruit. I've been waiting/hoping for a sporeless fruit to clone for quite some time but I'm concerned that because it's from such a slow colonizer that the negatives outweigh the positive.
Or, would it be possible through growing out several isolates of the new culture be able to further isolate a fast colonizing, sporeless culture from this fruit?
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Don't mistake my tone for a "matter-of-fact" attitude. I'm just presenting what I believe to be correct, until I'm corrected... - How Myco-Curious Prepares Coir & Compost Substrates - How Myco-Curious Builds A Bulk Humidifier - How Myco-Curious Builds An Automated Greenhouse ------------------------------------ figgusfiddus said: Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.
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YidakiMan
Stranger


Registered: 09/28/02
Posts: 2,023
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Re: Sporeless Mutant From Poor Performing Isolate? [Re: mycocurious]
#7618295 - 11/10/07 11:14 AM (16 years, 3 months ago) |
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It's probably not COMPLETELY sporeless. To find a better performing isolate, place gill fragments on agar and isolate. Not all isolates will still be sporeless.
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milkman
DeliveringWorldWide



Registered: 07/04/07
Posts: 2,108
Loc: tha FLA
Last seen: 3 months, 16 days
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Re: Sporeless Mutant From Poor Performing Isolate? [Re: YidakiMan]
#7619153 - 11/10/07 02:55 PM (16 years, 3 months ago) |
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why do you want a sporeless mushy cuase of the mess? and then how would you keep th species going for over 3-5 g2g/agar transfers?
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wutang
fungi



Registered: 06/28/07
Posts: 1,903
Last seen: 16 years, 2 months
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Re: Sporeless Mutant From Poor Performing Isolate? [Re: milkman]
#7621557 - 11/11/07 10:40 AM (16 years, 3 months ago) |
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i've had sporeless caps...100% sporeless i guess its just some shitty mutation
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legallyhomeless
mooch


Registered: 07/01/05
Posts: 4,051
Loc: EZRA for the Refuge
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Re: Sporeless Mutant From Poor Performing Isolate? [Re: wutang]
#7622743 - 11/11/07 05:12 PM (16 years, 3 months ago) |
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if i could get a sporeless mutant, i would clone it, then let the fruits get massive since they wont be trashing up my casing layer with spores
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boingkster
CuriousCultivator


Registered: 09/28/07
Posts: 109
Last seen: 14 years, 2 months
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Re: Sporeless Mutant From Poor Performing Isolate? [Re: legallyhomeless]
#7629026 - 11/13/07 06:14 AM (16 years, 3 months ago) |
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Make a master slant of the isolate you keep and you'll have no worries - just keep going back to it in the fridge. Man, you'll have some crazy shrooms...wonder if they'll be more prone to other mutations?
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