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Offlinewocka
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Which inoculation parameter
    #7621290 - 11/11/07 08:49 AM (16 years, 2 months ago)

i inoculated 6 pf-tek style jars about 3 days ago, and i ordered them online pre sterilized so all my lazy butt had to do was shoot spores in them.

now a few questions are, they looked kinda dry when they arrived, but i donno ive never see them before.

im keeping them in a garage thats usually around 85-86'f with about 50% humidity.

do they need higher humidity? im thinkin about putting half inside the house were its alot cooler to see which one grows better. what do u all think


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Offlineveda_sticks
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Re: Which inoculation parameter [Re: wocka]
    #7621312 - 11/11/07 09:00 AM (16 years, 2 months ago)

u don't need to worry about rh. u don't need that until u put them in ur fruiting chamber.

ur temps r too high. room temp is just fine no higher than 81_82.

ur jars should be fine. they should look like that. nice light fluffy colour. its better to be a little dry than wet. u will be dunking them after full colinisation anyway to rehydrate.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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Offlinewocka
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Re: Which inoculation parameter [Re: veda_sticks]
    #7621369 - 11/11/07 09:30 AM (16 years, 2 months ago)

sounds good thx for the advice. what if the room i put the jars in get cold like down to 74'f? the temp drops alot in the night since we are not using A/C and the cold fronts come threw.

i turned them upside down so the tin foil is on the bottom for better carbon dioxide exchange? there under a bed now were its 78


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Offlinewocka
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Re: Which inoculation parameter [Re: wocka]
    #7621574 - 11/11/07 10:46 AM (16 years, 2 months ago)

bump


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InvisibleNwerd
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Re: Which inoculation parameter [Re: wocka]
    #7621759 - 11/11/07 11:57 AM (16 years, 2 months ago)

Me personally... I suggest keeping them at the temps they are in.. In the jars, the substrate stays cooler than ambient temp... So if your garage is at 86... You are primo for incubation.. Once colonized and ready to fruit.. Get em in a 75 temp area and THEN offer them 100% humidity. Trust me, I've gotten ambient temps up to 90f and had no problem... 90 is not where you wanna KEEP it.. but it didn't hurt my operation :smile: Keep em where they are temp and humidity wise and keep em DARK. Drop the temp at fruiting... They'll get on super fast for ya :smile:


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Offlinewocka
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Re: Which inoculation parameter [Re: Nwerd]
    #7621780 - 11/11/07 12:10 PM (16 years, 2 months ago)

hmm. im gonna put half in the cooler room and half in the garage and see which one grows better since i have 2 opinions :smile:

should i have them upside down where the tin foil is on ground so the heavy co2 can escape easily? or just sit them tin foil side up, or sit them tinfoil side up with no tin on top so lil air holes can exchange air


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InvisibleNwerd
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Re: Which inoculation parameter [Re: wocka]
    #7621810 - 11/11/07 12:24 PM (16 years, 2 months ago)

Well... check it out.. Warmer climates will colonize quicker.. not to mention when you drop the temp to fruit... It will be more of a signal to fruit.. If you incubate in 80 and fruit in 75... There's isn't much of a difference for the myc to notice..
I've got 12 jars going right now.. Started a week and a day ago.. I've been incubating at 86 room temp and they are ALL ready to be spawned... When I started... I just let the temp vary between 85-75 throughout, I have definitely noticed the difference and I wouldn't incubate at anything less than 85f ROOM temp... It's just tooooo efficient to not do it that way.


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Offlineveda_sticks
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Re: Which inoculation parameter [Re: Nwerd]
    #7622020 - 11/11/07 01:34 PM (16 years, 2 months ago)

Quote:

Nwerd said:
Me personally... I suggest keeping them at the temps they are in.. In the jars, the substrate stays cooler than ambient temp... So if your garage is at 86... You are primo for incubation.. Once colonized and ready to fruit.. Get em in a 75 temp area and THEN offer them 100% humidity. Trust me, I've gotten ambient temps up to 90f and had no problem... 90 is not where you wanna KEEP it for definate (agree with u thre).. but it didn't hurt my operation :smile: Keep em where they are temp and humidity wise and keep em DARK. Drop the temp at fruiting... They'll get on super fast for ya :smile:




You are very wrong, myc will produce its own heat and can be 2-3 degrees hotter than the ambient temperature, That is why it is suggested to be no hotter than 81 F. Also room temperature is recommended as more often than not, incubation setups can be unreliable and end up with temperatures too hot causing problems.

Its better to leave them at room temperature than to have your incubator suddenly peak at 90F for a few hours each night.

If the ambient temp is 80 then when ur myc is producing its own little heat your jars could well be 82, it has been proven that myc growth slows down after 83 F.

Getting any hotter will just favour the growth of contams, this is to not say that it will stop contams.

Getting as low as 74F is fine, room temp is considered about 75F, the colders temps will slow it down a litte but will not do any harm.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


Edited by veda_sticks (11/13/07 06:24 AM)


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Offlinewocka
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Re: Which inoculation parameter [Re: veda_sticks]
    #7622060 - 11/11/07 01:49 PM (16 years, 2 months ago)

my experiment will soon tell which grows mycelium better :>

ill let yall know in about a week

its wierd that 2 people swear by the exact same thing?


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Offlinewocka
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Re: Which inoculation parameter [Re: wocka]
    #7627815 - 11/12/07 08:27 PM (16 years, 2 months ago)

my mycelium seems to grow in the hotter temperature aposed to room temps.


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Offlineveda_sticks
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Re: Which inoculation parameter [Re: wocka]
    #7629043 - 11/13/07 06:33 AM (16 years, 2 months ago)

Mycelium will grow faster in the optimal temperature range, but its not gonna grow that much faster, if u have built a good incubator that is garenteed to stay at the set temp, then fine.

Most of the professional growers out here will advise against incubating and themselfs do not incubate, and for very good reasons.

Since starting i have never incubated, the only time i had jars take ages to colinise was with a slightly wet substrate that had gotten a little compacted.

My last few jars had to get moved to a colder room (around 60F)and they done just fine.

On that note, i have even been fruiting in lower temps 60-70F.

Wocka, yes mycelium will grow faster in the higher temps, but its not gonna be much faster than room temp.

I seem to remember RR saying that he has grown jars at 75F in just over 14 days. (but dont quote me on that)

Also, the temp drop is one of the least important pinning triggers.

FAE is the most important followed by humidity and light.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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OfflineRogerRabbitM
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Re: Which inoculation parameter [Re: veda_sticks]
    #7629087 - 11/13/07 06:56 AM (16 years, 2 months ago)

Temp drop is not a pinning trigger with cubensis at all, and you're correct, mycelium produces heat as it colonizes a substrate, making the interior of the jars warmer than ambient.
RR


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Invisiblefastfred
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Re: Which inoculation parameter [Re: RogerRabbit]
    #7630968 - 11/13/07 02:52 PM (16 years, 2 months ago)

> Most of the professional growers out here will advise against incubating and themselves do not incubate, and for very good reasons.

Where are you getting this from? This site is loaded with elaborate incubation setups created by expert growers.

There is no reason not to use them except for time/cost considerations and the fact that once you get going you'll have a rotation which will cause incubation time to be a non-factor.

The difference between room temp (72F) and say 84F is quite noticeable.

Optimal growth parameters are around 85-86 from what I've always read. So given that the jars generate a heat of 1-3 degrees, your optimal incubation temp is going to be about 83F.

From Stamets' 'The Mushroom Cultivator':

I know the graph is a pretty shitty scan, but you can clearly see that 86F is the optimal temperature for growth. I assume this was constructed using petri dishes in an incubator, so it's safe to say that you need to subtract 1-3F to account for the heat generated by the myc.


-FF


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Offlinefuturewolf42o
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Re: Which inoculation parameter [Re: fastfred]
    #7631025 - 11/13/07 03:07 PM (16 years, 2 months ago)

theres lots of discussion on the BEST temps. but in reality myc will grow anywhere from 70-90 degrees. fruiting temps seem to be more important than colonizing temps. although getting the right temp will make your myc grow efficiently.


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Offlinewocka
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Re: Which inoculation parameter [Re: futurewolf42o]
    #7632705 - 11/13/07 09:13 PM (16 years, 2 months ago)

the 3 jars that were in room temp showed 80% decrease in growth compared to the incubated ones. cold hard facts that a good stable high temp beats out any room temp.

can some one pm me if they know how i can incubate more jars with the colonized jar or a piece of mushroom so i got infinit supply


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