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Invisiblebudmanman
OTD Masterbater
Male


Registered: 02/07/07
Posts: 17,975
Loc: PNW
8 of 9 jars made it, but why.
    #7618481 - 11/10/07 12:05 PM (16 years, 2 months ago)

So I knocked up 9 jars with some PE uncut. I did make 10 but one cracked in the pc. But all 8 jars colonized supper fast, but the one never did anything.... I gave them all 1cc equally.


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Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal.

And I am mentally unstable.


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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: 8 of 9 jars made it, but why. [Re: budmanman]
    #7618758 - 11/10/07 01:16 PM (16 years, 2 months ago)

the world may never know. thats not bad though, you got 8 jars.


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Offlineshroom_ninja
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Registered: 11/03/07
Posts: 150
Last seen: 8 years, 10 months
Re: 8 of 9 jars made it, but why. [Re: blood4blood]
    #7619008 - 11/10/07 02:11 PM (16 years, 2 months ago)

Did you shake the syringe well? (assuming you used one) before inoculating?

If you flame-sterilized the needle, did you get rid of the liquid that you basically burned (and a little extra, to cool the needle).. ?

Did you mix your substrate in between jars? If not, the last few jars can have a much higher water content. I usually mix my substrate well every few jars to avoid that.

I've had this happen to me a lot using spore syringes from companies. The problem becomes a lot less common once you begin using your own spore prints and/or agar samples.


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Offlineveda_sticks
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Registered: 07/29/07
Posts: 14,191
Loc: UK Flag
Last seen: 4 years, 25 days
Re: 8 of 9 jars made it, but why. [Re: shroom_ninja]
    #7621153 - 11/11/07 06:48 AM (16 years, 2 months ago)

Thats a pretty good success rate, so i wouldnt worry too much about it, it may have been unlucky and got a bacterial contam, or spores somehow didnt make it.


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Offlinenerotheavenger
turd that won'tflush
Registered: 11/11/07
Posts: 51
Last seen: 14 years, 9 months
Re: 8 of 9 jars made it, but why. [Re: budmanman]
    #7621277 - 11/11/07 08:38 AM (16 years, 2 months ago)

seriously i would not worry. you got 8 of 9. could be too many things to consider specially with that success rate.


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