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OfflineNem
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Registered: 09/29/07
Posts: 144
Last seen: 13 years, 6 months
LC contam question, just worried
    #7609174 - 11/07/07 10:28 PM (16 years, 2 months ago)

So I made 3 LC jars and put 1 cc of B+ spore solution from ralphsters in each one. That was about a week ago. Right now I have some little poofs of white stuff chilling at the bottom that I mix up a few times a day. I dropped some new nuts and bolts in as an agitator, I don't have a stir plate yet.

A couple days ago I was stirring one of them and the micropore tape got wet so I set that one aside and am considering it a loss, am I correct in this?

My other concern is that a few of the white poofs have black dots in the middle. They are in both of the jars that I was sure were safe but not in the one that I thought could be suspect to contamination from the wet micropore tape.

I am really hoping they aren't contaminated because I only have 1 cc left of spore solution in the syringe after making those 3 jars and 6 pf cakes. And if the cakes that I just got done cold dunking, crumbling and casing yesterday don't fruit and these are contaminated I will be out of genetic material to try again.


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OfflineCiv
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Registered: 10/14/04
Posts: 2,537
Loc: California
Last seen: 6 months, 17 days
Re: LC contam question, just worried [Re: Nem]
    #7609182 - 11/07/07 10:32 PM (16 years, 2 months ago)

The black dots, if they don't grow sometimes are just spore clumps that are easy to see with a myc background. You should be fine. The LC touching the tyvek is not gonna ruin you. It just can increase your chance of contamination.


--------------------
"...Gal's seem to hate the thought of blending chicken shit in a blender.
So, wash it well afterwards & DON'T tell them..."  -Agar


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OfflineNem
Stranger
Registered: 09/29/07
Posts: 144
Last seen: 13 years, 6 months
Re: LC contam question, just worried [Re: Civ]
    #7609209 - 11/07/07 10:38 PM (16 years, 2 months ago)

Alright, thanks. I am just a bit worried because it is my first time and all. I am taking some pictures right now, you can't really see the dots but you can see the white poofs to make sure those look kosher.


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OfflineNem
Stranger
Registered: 09/29/07
Posts: 144
Last seen: 13 years, 6 months
Re: LC contam question, just worried [Re: Nem]
    #7609460 - 11/07/07 11:41 PM (16 years, 2 months ago)

http://img209.imageshack.us/img209/7361/1107072336wx0.jpg
http://img209.imageshack.us/img209/8531/1107072337uq1.jpg

Sorry the pictures aren't great, they are taken from a camera phone, it's all I have.


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Offlineyepyepyep
Stranger
Registered: 09/05/07
Posts: 147
Last seen: 26 days, 7 hours
Re: LC contam question, just worried [Re: Nem]
    #7609683 - 11/08/07 01:03 AM (16 years, 2 months ago)

The white poofs look fine. The black dots I would watch. If they increase you're probably facing contamination.


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