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anarchOi
Ellenalien is fat.



Registered: 08/06/07
Posts: 2,293
Loc: ASE
Last seen: 12 years, 11 months
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tub size?
#7609106 - 11/07/07 10:06 PM (16 years, 2 months ago) |
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I've been looking around for the correct spawn->substrate ratios and i believe i've decided on 1->4
i have 9 nearly full pint jars full of rye berries, so like 2.2-2.4quarts of spawn i'm gonna be using cpoo/straw/verm for substrate and i would like 3-4 inches of substrate
i'm not a math wizz, so my question is what size tub should i use? i was thinking an 18 gallon rubbermaid, but some of the things i've read have made me question whether 18gs might be a little large for only 2.4quarts of spawn
i've isolated a very strong ecuador clone on BRF-substitute agar that has been performing exceptionally well all 9 jars are within a few days of full colinization after a week with a single point inoculation (agar wedge)
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mycocurious
Mike O. Kuerias



Registered: 02/09/07
Posts: 1,265
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Re: tub size? [Re: anarchOi]
#7609120 - 11/07/07 10:10 PM (16 years, 2 months ago) |
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randomly, if you need 9 quarts of spawn then you'll need to prepare inoculate 12 jars to get it because you never want to fill up the jars more than 75% (50% if they're going to receive grains on a grain-2-grain transfer) full...to allow room for growth and to make shaking easier.
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Don't mistake my tone for a "matter-of-fact" attitude. I'm just presenting what I believe to be correct, until I'm corrected... - How Myco-Curious Prepares Coir & Compost Substrates - How Myco-Curious Builds A Bulk Humidifier - How Myco-Curious Builds An Automated Greenhouse ------------------------------------ figgusfiddus said: Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.
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anarchOi
Ellenalien is fat.



Registered: 08/06/07
Posts: 2,293
Loc: ASE
Last seen: 12 years, 11 months
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well i filled each jar up to the line just below the rim of the lid with rye berries because i made alot more than i intended to leaving about an inch of space between the rye and the lid, just barely enough to shake them up
i shook them a few times during the growth process because of the small innoc point, it worked out well since the isolate is so vigorous
i unfortunately don't have a good pc'r so i can't PC anything more than 3 pint jars at a time (if i get the bulk going well then that'll change xD)
i think you meant 9 pints though
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Civ
Pinning



Registered: 10/14/04
Posts: 2,537
Loc: California
Last seen: 6 months, 18 days
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Re: tub size? [Re: anarchOi]
#7609191 - 11/07/07 10:34 PM (16 years, 2 months ago) |
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don't overfil, an inch is not enough on qts, 2/3s to 3/4s full at most. With G2G I advise even less.
-------------------- "...Gal's seem to hate the thought of blending chicken shit in a blender. So, wash it well afterwards & DON'T tell them..." -Agar
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anarchOi
Ellenalien is fat.



Registered: 08/06/07
Posts: 2,293
Loc: ASE
Last seen: 12 years, 11 months
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Re: tub size? [Re: Civ]
#7609206 - 11/07/07 10:37 PM (16 years, 2 months ago) |
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as i said i didn't intend to make so much rye, but i knew it would work with the isolate, so i went with it and now i have nearly fully colonized jars with 0% contamination (knock on wood)
that's not the question though guys, what i need to know is what size tub should i use to obtain 4inches of substrate with 2.4quarts of spawn using a 1->4 spawn->substrate ratio
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anarchOi
Ellenalien is fat.



Registered: 08/06/07
Posts: 2,293
Loc: ASE
Last seen: 12 years, 11 months
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Re: tub size? [Re: anarchOi]
#7611166 - 11/08/07 01:12 PM (16 years, 2 months ago) |
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any ideas?
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