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Offlineresptodd
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Registered: 10/16/07
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lc from a pf cake
    #7608901 - 11/07/07 09:14 PM (16 years, 2 months ago)

Can someone lead me in the direction. I want to try and pull out some myc. from one of my pf jars and transfer it to LC.


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InvisibleRoachMan
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Re: lc from a pf cake [Re: resptodd]
    #7608929 - 11/07/07 09:20 PM (16 years, 2 months ago)

#1) USE A GLOVEBOX

#2) If you don't have a glovebox...don't even mess with it.

#3) If you DO, and know how to use it:

Inside the glovebox...
Open the jar, and flip the entire cake upside down on the lid.
Use previously sterilized scalpel to cut a tiny piece out,
and deftly move it to the LC jar while keeping it closed 9/10ths
of the way yet ensuring that the extracted myc lands onto the LC
surface.


Reseal the LC container quickly, and move to a warm place.

IMO, it's better to clone from a fruitbody or start an LC from a
reptuable vendor syringe.


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OfflineCaptainLinger
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Re: lc from a pf cake [Re: RoachMan]
    #7608941 - 11/07/07 09:24 PM (16 years, 2 months ago)

You're really not going to want to do that. Cakes aren't really sterile anyhow. You have to dump out a bunch of presumably spore-laden dry vermiculite to get to anyway.

A cake is bound to have some contaminants, and transferring to an LC is shaky at best. I wouldn't advise it. Yes, use a glovebox.


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Offlineresptodd
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Re: lc from a pf cake [Re: CaptainLinger]
    #7609004 - 11/07/07 09:41 PM (16 years, 2 months ago)

So, this is not a common practice I take it. And probably not something I want to try.----Then, I have a slow growing pf cake which I am willing to sacrifice, it's half colonized. Are there any tecs in which I can use that half colonized pf cake to inoc. something, if anything else?


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OfflineCaptainLinger
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Re: lc from a pf cake [Re: resptodd]
    #7609320 - 11/07/07 11:00 PM (16 years, 2 months ago)

No, you definitely don't. It's also worth noting that if it's growing particularly slowly, that's a sign of likely contamination or poor genetics. When you inoculate with spores, you initiate sexual reproduction that produces genetic variance; liquid cultures and mycelium transfers will duplicate genetic tratits. So you'd rather not go around using a notably slow-growing mycelium.

A better option is to take your most promising fruit of a cake, and take a core sample with a sterilized biopsy needle. The process is simple, and very shielded; you'll preserve the genetics of your best grower in perpetuity.


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OfflineCaribou_Lou
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Re: lc from a pf cake [Re: CaptainLinger]
    #7609336 - 11/07/07 11:04 PM (16 years, 2 months ago)

You could always soak the cake with a 10% bleach solution prior to doing this, to make sure there are no contams.


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OfflineCaptainLinger
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Re: lc from a pf cake [Re: Caribou_Lou]
    #7609606 - 11/08/07 12:26 AM (16 years, 2 months ago)

That would kill any mycelium it comes into contact with as well. Not a great plan.


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OfflineGuffeyJon
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Re: lc from a pf cake [Re: CaptainLinger]
    #7609657 - 11/08/07 12:53 AM (16 years, 2 months ago)

if its a 10% mix of bleach it wouldn't hurt healthy mycelium, some people ever swear it helps.(I'm not one of them) I think there is a tek with grain where you make a lc put some in your jar and some in a syringe sterilize both, now inject your jar with the lc syringe shake a suck the liquid back out and into your lc. that will work with grain, and could work with brf cake. i would try to use a 100% colonized cake, be careful of the dry verm on the top, and birth cake after you make the lc.


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Offlinerodfarva
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Re: lc from a pf cake [Re: GuffeyJon]
    #7609671 - 11/08/07 12:59 AM (16 years, 2 months ago)

check out THIS thread brother.


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OfflineCaptainLinger
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Re: lc from a pf cake [Re: rodfarva]
    #7609784 - 11/08/07 01:53 AM (16 years, 2 months ago)

It's most certainly possible. The theory behind it isn't wrong. It's just begging for contamination though; there are simpler ways to do this.

Using bleach as a substitute for clean culture (which will work just fine) isn't really a great plan.


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Invisiblebudmanman
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Registered: 02/07/07
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Re: lc from a pf cake [Re: CaptainLinger]
    #7609788 - 11/08/07 01:57 AM (16 years, 2 months ago)

I made my first LC successfully this way and had my first successfully bulk grow.

Now as an experienced grower, IT WAS A FLUKE!

but you could get lucky!


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