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trippindad82
Trusted Cultivator of Trich



Registered: 01/07/07
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Question about trays/tubs
#7606431 - 11/07/07 11:09 AM (16 years, 2 months ago) |
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My friend is going to be doing a bunch of tubs up tonight. His grow room is heated to 74-78 degrees with an average humidity of 60%. Is this room an acceptable place to incubate colonizing tubs or will this cause them to dry out like an incubator would dry out trays?
Also, there are two or three jars that are fully colonized minus a grain or two of rye pressed up against the side of the jar. These jars have been the same way for two days now, so I don't know if those grains will ever colonize. Will it be safe to spawn these jars?
-------------------- Trying to explain a journey to someone who has never experienced it is like trying to explain what a zebra looks like to blind person who has never seen a horse. ^^^The above matter may be a complete fantasy that I concocted out of possible boredom.^^^ --------------------------------------
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mycocurious
Mike O. Kuerias



Registered: 02/09/07
Posts: 1,265
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Re: Question about trays/tubs [Re: trippindad82]
#7606466 - 11/07/07 11:15 AM (16 years, 2 months ago) |
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search "shot gun terrarium"

create a terrarium like that for those trays and they will flourish in that envrionment...
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Don't mistake my tone for a "matter-of-fact" attitude. I'm just presenting what I believe to be correct, until I'm corrected... - How Myco-Curious Prepares Coir & Compost Substrates - How Myco-Curious Builds A Bulk Humidifier - How Myco-Curious Builds An Automated Greenhouse ------------------------------------ figgusfiddus said: Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.
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trippindad82
Trusted Cultivator of Trich



Registered: 01/07/07
Posts: 1,087
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Last seen: 10 years, 11 months
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Re: Question about trays/tubs [Re: mycocurious]
#7606509 - 11/07/07 11:25 AM (16 years, 2 months ago) |
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I have a terrarium. I'm trying to clarify RR's statement. I know he recommends incubating the trays on a shelf at room temperature. The problem is that the average room temp at my friend's place is 60-65 degrees, far lower than what's recommended. One room in his place is heated up around 74-78 degrees and he is using that one as his grow room. Is this room acceptable to leave colonizing trays? He doesn't want to put them where he incubates his jars, as last time that ruined his project, and also because RR says not to do that, for the reasons we encountered.
-------------------- Trying to explain a journey to someone who has never experienced it is like trying to explain what a zebra looks like to blind person who has never seen a horse. ^^^The above matter may be a complete fantasy that I concocted out of possible boredom.^^^ --------------------------------------
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sublimemushhead
researcher



Registered: 01/17/07
Posts: 223
Loc: New Mimzy Galaxy
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Re: Question about trays/tubs [Re: trippindad82]
#7606965 - 11/07/07 01:21 PM (16 years, 2 months ago) |
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I always incubate using a cool mist enclosed environment @ approx.80*-86* Never dries out and no contams. Seriously.
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mycocurious
Mike O. Kuerias



Registered: 02/09/07
Posts: 1,265
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I incubate my rye grains and bulk substrates in the 68-72(F) range because my wife loves to keep the house freezing cold. Incubation takes a couple days long but cuts down on a lot of potential bacterial contaminations - as they don't flourish until the temps reach the 80's...
Additionally, because the mycelium generate their own heat internally, in order to keep them at or below their threshold of 82(F) - at which colonization slows down because the heat is stressing the colony - you'll have to keep the ambient temperatures lower. Up to 10(F) degrees lower in the case of a bulk substrate as the large volume of material can put of a (relatively) large amount of heat...
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Don't mistake my tone for a "matter-of-fact" attitude. I'm just presenting what I believe to be correct, until I'm corrected... - How Myco-Curious Prepares Coir & Compost Substrates - How Myco-Curious Builds A Bulk Humidifier - How Myco-Curious Builds An Automated Greenhouse ------------------------------------ figgusfiddus said: Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.
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sublimemushhead
researcher



Registered: 01/17/07
Posts: 223
Loc: New Mimzy Galaxy
Last seen: 14 years, 1 month
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Re: Question about trays/tubs [Re: mycocurious]
#7607914 - 11/07/07 05:10 PM (16 years, 2 months ago) |
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82* or 86*? My research has lead me to believe that diminished growth is started @87*. 76* is my optimal giving a safe 10* net for the substrates generated heat. But usualy stays about 80*. Never any trouble w/ contams though.
Edited by sublimemushhead (11/07/07 05:12 PM)
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trippindad82
Trusted Cultivator of Trich



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My friend thanks you all for the help. Now one thing he was unsure of was how sanitary the space had to be. Is it true that properly pasteurized coir is contam resistant? Obviously, he took the proper precautions, but doing the work out in the open air of a clean room shouldn't lead to contamination, right?
Thanks for the help.
-------------------- Trying to explain a journey to someone who has never experienced it is like trying to explain what a zebra looks like to blind person who has never seen a horse. ^^^The above matter may be a complete fantasy that I concocted out of possible boredom.^^^ --------------------------------------
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anarchOi
Ellenalien is fat.



Registered: 08/06/07
Posts: 2,293
Loc: ASE
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Re: Question about trays/tubs [Re: trippindad82]
#7609136 - 11/07/07 10:15 PM (16 years, 2 months ago) |
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incubating does help.. i have 2 incubators, one is a shipping styrofoam cooler(the kind they use to send temp sensitive parcels) with a heatbomb inside which since it's so well insulater stays about 80degrees, then the 2nd one is simply a box folded closed which stays around 74 degrees or so
i usually put the rye jars that are too far ahead of their brothers in the lower temp one to slow growth a little bit, and the ones being slow into the better insulated one
this usually equalizes them
while incubating isn't really neccesary, it deff helps
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trippindad82
Trusted Cultivator of Trich



Registered: 01/07/07
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Re: Question about trays/tubs [Re: anarchOi]
#7610079 - 11/08/07 07:30 AM (16 years, 2 months ago) |
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Bump.
If the coir was properly prepared, then the risk of contam while spawning in the open air is nill, correct?
-------------------- Trying to explain a journey to someone who has never experienced it is like trying to explain what a zebra looks like to blind person who has never seen a horse. ^^^The above matter may be a complete fantasy that I concocted out of possible boredom.^^^ --------------------------------------
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Slimz
.-~*´`*·~-experience-~*´`*·~-.




Registered: 10/03/07
Posts: 3,588
Loc: Maryland
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Re: Question about trays/tubs [Re: trippindad82]
#7610094 - 11/08/07 07:43 AM (16 years, 2 months ago) |
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Spawning in open air ALWAYS risks contam
-------------------- Lazy Drywall Tek (no powdery mess) This series will blow your mind and confirm what you already know to be true. The Pharmacratic Inquisition Best Thread Ever ! ! !
me if you have questions about lasers Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles. FeelFamily resident tech guru
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trippindad82
Trusted Cultivator of Trich



Registered: 01/07/07
Posts: 1,087
Loc: down, down the hole
Last seen: 10 years, 11 months
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Re: Question about trays/tubs [Re: Slimz]
#7610135 - 11/08/07 08:09 AM (16 years, 2 months ago) |
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I came across this from RR.
Quote:
Once your spawn jars are fully colonized, you can scratch your butt while you inoculate the coir if you want. Bacteria isn't a contaminant of bulk substrates. The important thing is to learn when it's important to be sterile.
So, that helps clear it up for me. I thought I had read before that it isn't necessary to be that sterile when spawning. But, being that this is my friends first "bulk" tray, we wanted to make sure. Super paranoid.
-------------------- Trying to explain a journey to someone who has never experienced it is like trying to explain what a zebra looks like to blind person who has never seen a horse. ^^^The above matter may be a complete fantasy that I concocted out of possible boredom.^^^ --------------------------------------
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anarchOi
Ellenalien is fat.



Registered: 08/06/07
Posts: 2,293
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Last seen: 12 years, 11 months
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Re: Question about trays/tubs [Re: trippindad82]
#7610146 - 11/08/07 08:18 AM (16 years, 2 months ago) |
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a question that i've been wondering about is if bulk is contam resistant, would it be ok to stir up your substrate at 40% colonization to speed up full colonization?
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mycocurious
Mike O. Kuerias



Registered: 02/09/07
Posts: 1,265
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Re: Question about trays/tubs [Re: anarchOi]
#7610420 - 11/08/07 10:07 AM (16 years, 2 months ago) |
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the only thing that makes a bulk substrate contaminate resistant is the fact that it was properly pasteurized and was introduced to a healthy, uncontaminated spawn (your grains, crumbled cakes, etc.) run that was already 100% colonized. The pasteurization process kills any fungal contaminates and any mesophilic bacterial contaminates. Only the thermophlic bacteria - which are considered the _beneficial_ bacteria - can survive the temperatures of proper pasteurization. These thermophilic bacteria have been linked to pinning triggers - although i can't quote a cite at the moment - as well as assisting in staving off invader bacterial infections.
--- And now, for something completely different...
The *other* reason why RR mentions that bacterial infections are not a "contaminate" for bulk trays is because at the point in which they the spawn run is initiated, there is so much healthy mycelium colonizing the substrate it would take something short of a miracle for a bacterial infection to win out the "substrate consolization war" being waged by your mycelial army that's just landed...it will win and it will fruit. It doesn't meant that an improperly pasteurized substrate will be infection free, it very well could have some type of bacterial infection that may cause food-poisoning, but the colony will still fruit virtually unaffected...
In that regard, bulk substrates are contaminate resistant.
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Don't mistake my tone for a "matter-of-fact" attitude. I'm just presenting what I believe to be correct, until I'm corrected... - How Myco-Curious Prepares Coir & Compost Substrates - How Myco-Curious Builds A Bulk Humidifier - How Myco-Curious Builds An Automated Greenhouse ------------------------------------ figgusfiddus said: Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.
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