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johnnyboy
Bastard Son

Registered: 04/02/07
Posts: 644
Last seen: 11 years, 8 months
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innoculating rye
#7606203 - 11/07/07 10:12 AM (16 years, 2 months ago) |
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im about ready to inoculate some rye with multispore. i was wondering how this works with my jars being only 2/3 full and only having a 1 1/2" needle. my lids will have 4 holes in them.
do i just put the needle through the hole as far as it can go against the glass (if it can reach) and just squirt 3/4 cc of the solution against the glass and let it run down into the grains?
does this pose a contamination risk with having spores on the glass?
can i just squirt directly down on top of the grains? does it have to be against the glass?
thanks
-------------------- I keep the bible in a pool of blood so that none of it's lies will affect me
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urayasan
Stranger

Registered: 04/10/06
Posts: 111
Last seen: 16 years, 27 days
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Re: innoculating rye [Re: johnnyboy]
#7606274 - 11/07/07 10:28 AM (16 years, 2 months ago) |
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You don't have to inject against the glass, but it is good for a couple reasons. First it allows the majority of the solution to pool in the bottom, rather than being filtered by all the grains in the middle, this allows the spores to germinate quickly because it is faster for compatible pairs to meet.
The second good thing is that you see growth quicker as it is against the glass, and not in the center of your grains. You can quickly assess growth and hopefully spot contaminations faster if they are introduced by the syringe.
Just aim the needle against the glass as well as you can, and do your best not to come in contact with any grains.
Good luck!
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Magash
Da Bud Guru



Registered: 07/25/02
Posts: 5,876
Loc: Near Hilo
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Re: innoculating rye [Re: johnnyboy]
#7608551 - 11/07/07 07:42 PM (16 years, 2 months ago) |
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I use to just make one hole and aim toward the glass in a few spots and then shake the shit out of it.
3 days later 
9 days later
-------------------- All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?
Join us at the Growery!
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BUDDHA_702
Master Mycologist In Training



Registered: 02/17/07
Posts: 1,296
Loc: Some Country
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Re: innoculating rye [Re: Magash]
#7608625 - 11/07/07 08:00 PM (16 years, 2 months ago) |
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^^how many CC's did you use?
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: innoculating rye [Re: BUDDHA_702]
#7609056 - 11/07/07 09:53 PM (16 years, 2 months ago) |
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i think 2-3 cc's will suffice.
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