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OfflineSpooglywoogly
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Registered: 11/06/07
Posts: 38
Last seen: 15 years, 4 months
Another First Timer Stepping Out Into Cultivation
    #7605036 - 11/06/07 10:18 PM (16 years, 2 months ago)

Well!

The wheels are finally in motion. I have 2 spore syringes. 1 of b+ and another of huautla. i've spent the best part of 4 hours trotting around my kitchen in the dead of night like a cross between Dr.Frankenstein and Delia Smith. I felt quite classy in using some rather nice cut glass whiskey tumblers to put the cakes in.

Having planted and cooked the little fellas i thought i would be inoculating straight away. But it dawned on me, as i was preparing the jacking-up area with disinfectant and dub, that shooting the spores into near to boiling substrate was perhaps not a good idea.

As everyone keeps saying: patience is the key!

I feel something akin with the feeling u get after mail ordering an obscure gift. elation and premature anticipation. Its time to settle back and wait. no amount of rushing around and personal haste is gonna speed the process up. things must brew!

ive collected a few crumby pics which i shall upload as i go along. but for now...

[][][][][] !STAGE ONE COMPLETE! [][][][][]


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OfflineSeventy
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Registered: 05/24/07
Posts: 912
Loc: Canada (PNW)
Last seen: 6 months, 28 days
Re: Another First Timer Stepping Out Into Cultivation [Re: Spooglywoogly]
    #7605077 - 11/06/07 10:25 PM (16 years, 2 months ago)

good luck on your grow!

you'll be smiling even more soon enough.


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OfflineSpooglywoogly
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Registered: 11/06/07
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Last seen: 15 years, 4 months
Re: Another First Timer Stepping Out Into Cultivation [Re: Seventy]
    #7605086 - 11/06/07 10:27 PM (16 years, 2 months ago)

hehe, i hope so.

fingers crossed the inoculation goes well...

reading contamination reports doesn't fill me with confidence.


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Offlinec0_hush
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Re: Another First Timer Stepping Out Into Cultivation [Re: Spooglywoogly]
    #7605110 - 11/06/07 10:33 PM (16 years, 2 months ago)

Where did you order the spores from and how long did it take if you don't mind...


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OfflineAzurescendence
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Registered: 10/21/07
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Re: Another First Timer Stepping Out Into Cultivation [Re: Spooglywoogly]
    #7605115 - 11/06/07 10:33 PM (16 years, 2 months ago)

I'll be starting on my own soon as well, so I know the feeling! lol

Good luck! - and with that patience and the tons of good information here I'm sure you'll be enjoying the fruits of your labor soon enough :grin::mushroom2:


--------------------
~ Peace, love, happiness, and good health to all. ~


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OfflineNewFungiGrower
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Registered: 08/11/06
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Last seen: 15 years, 5 months
Re: Another First Timer Stepping Out Into Cultivation [Re: Spooglywoogly]
    #7605121 - 11/06/07 10:34 PM (16 years, 2 months ago)

The single best advice I can give you. Please do your self a fav and make a liquid culture out of your spore syringe. It is not that hard and it will work a hell of a lot better. And you will have that strain forever. Start collecting =]


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OfflineSeventy
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Re: Another First Timer Stepping Out Into Cultivation [Re: NewFungiGrower]
    #7605122 - 11/06/07 10:35 PM (16 years, 2 months ago)

yeah, LCs are time savers and money savers.

do your self a favor and make one


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Invisiblemycocurious
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Registered: 02/09/07
Posts: 1,265
Re: Another First Timer Stepping Out Into Cultivation [Re: Seventy]
    #7605148 - 11/06/07 10:42 PM (16 years, 2 months ago)

I mistrust them LC's because most contaminate mycelium appears _very_ similar to your specific mycelium. It's much more cost effective to grow out a quart of rye grains and see any contaminates than inoculate a reasonable volume of bulk substrate only to discover the entire inoculate was contaminated from the go.


--------------------
:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

- How Myco-Curious Prepares Coir & Compost Substrates
- How Myco-Curious Builds A Bulk Humidifier
- How Myco-Curious Builds An Automated Greenhouse
------------------------------------
figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.


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OfflineNewFungiGrower
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Re: Another First Timer Stepping Out Into Cultivation [Re: mycocurious]
    #7605152 - 11/06/07 10:44 PM (16 years, 2 months ago)

WRONG if you have a contam LC, you will know it far before you use it. If you wait a reasonable time that is.


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OfflineSpooglywoogly
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Re: Another First Timer Stepping Out Into Cultivation [Re: c0_hush]
    #7605160 - 11/06/07 10:46 PM (16 years, 2 months ago)

i got mine from here: <removed url>

they took 3 days to arrive i think, something like that. There are other cheaper sites around for UK folk but they didn't accept my card. so i had to go with them.

edit: i removed the link because of a later comment.


Edited by Spooglywoogly (11/07/07 06:22 AM)


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OfflineSpooglywoogly
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Re: Another First Timer Stepping Out Into Cultivation [Re: NewFungiGrower]
    #7605169 - 11/06/07 10:49 PM (16 years, 2 months ago)

i've not heard of the liquid culture approach. hmmmm. you guys reckon its definately the better option? i guess id better get back to trawling the forum :smile:

quick edit:

ive had a look through this guide: http://www.shroomery.org/forums/showflat.php/Number/4141612#Post4141612

So a liquid culture is a solution where u've already given the mycelium time to take hold and form. therefore your far less likely to suffer contamination as theres very little down time from inoculation to mycelium formation and consumption of the substrate.

that make sense?
heh


Edited by Spooglywoogly (11/06/07 11:00 PM)


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OfflineSkeeblix
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Registered: 01/28/07
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Re: Another First Timer Stepping Out Into Cultivation [Re: Spooglywoogly]
    #7605233 - 11/06/07 11:13 PM (16 years, 2 months ago)

That's exactly how LC works, though I'd recommend getting a regular PF grow under your belt first. Learn proper sterile technique before you use a whole syringe on an LC, as it's pretty hard to tell if it's contaminated unless you use it to inoculate a few jars with it. At least with cakes, you can tell when something funky starts growing.

Baby steps help. Some people jump right out into stuff like casing, but generally, getting your feet wet with something simple like PF is a good way to start. If you really think you've read enough (which is pretty much impossible with this hobby) to try something more advanced for your first grow, give it a shot, but don't be too surprised if it all turns green. If it doesn't, more power to you! Just remember that this isn't the simplest of things to do. It's not exactly hard, but it's a hobby that involves combating stuff floating in the air that's so small you can't see it.

Patience is another key thing. Rushing can ruin a grow. I've heard of a few noobs who didn't take the time to CAREFULLY read a tek and then shot up substrate fresh out of the PC.

Just read as much as you can find about everything here. There will always be something new to learn.


--------------------
This post approved by:


Premedman1 said:
:lol: I just shat my pants.


Edited by Skeeblix (11/06/07 11:15 PM)


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OfflineNewFungiGrower
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Re: Another First Timer Stepping Out Into Cultivation [Re: Skeeblix]
    #7605253 - 11/06/07 11:20 PM (16 years, 2 months ago)

you dont have to waste a whole syringe on an lc just a little squeeze. And if you can mix up and PC brf then you can mix up and PC a karo/water solution.


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Offlineveda_sticks
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Re: Another First Timer Stepping Out Into Cultivation [Re: NewFungiGrower]
    #7605703 - 11/07/07 06:04 AM (16 years, 2 months ago)

U cant mention non shroomery sponsors here. Only buy from shroomery sponsers, they are trusted.

I got hit by card fraud using a non shroomery sponser, and i also got a syringe that didnt have any spores in it.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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OfflineSpooglywoogly
swoooooosh
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Registered: 11/06/07
Posts: 38
Last seen: 15 years, 4 months
STAGE TWO [Re: Spooglywoogly]
    #7605861 - 11/07/07 08:00 AM (16 years, 2 months ago)

After getting to sleep at 6am i woke up feeling groggy and tired. After a coffee i went about the inoculation.

(i decided not to bother with the liquid culture this time round)

i had 2 10cc syringes.

im not overly proud of my sterilization technique but i dont have vast amounts of cash to spend on the gear.

The process went something like this:


  • Washed my hands with anti-bacterial handwash.

  • Disinfected my desk using all purpose anti-bacterial spray.

  • Wiped clean and dry.

  • Took the syringe out of the packet and wiped the tip with a cloth drenched in 45% vodka (the vodka im not sure about)

  • Carefully lifted the top layer of foil and stood it face up on my desk.

  • Heated the tip of the syringe with a cigarette lighter for 30secs until it was clearly red hot.

  • Injected 0.3cc into 4 points in the foil.

  • Replaced the foil lid and wrote the appropriate strain on the top.



If anyone can see any drastic mistakes with this method please let me know.

After inoculation was complete on all 16 jars i placed them inside a cupboard in my bathroom. (again if this isn't a good idea please let me know)

I shall find out in a week or so whether my efforts were enough.

[][][][][] !STAGE TWO COMPLETE! [][][][][]


Edited by Spooglywoogly (11/07/07 08:54 AM)


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OfflineSpooglywoogly
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Re: Another First Timer Stepping Out Into Cultivation [Re: Spooglywoogly]
    #7607432 - 11/07/07 03:30 PM (16 years, 2 months ago)

gonna bump this to glean replies to my last post...

bump!


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OfflineFraggin
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Re: Another First Timer Stepping Out Into Cultivation [Re: Spooglywoogly]
    #7607444 - 11/07/07 03:33 PM (16 years, 2 months ago)

The only drastic mistake, was assuming that it takes vast amounts of cash to spend on gear.

A glove box made with a cardboard box and saran wrap will decrease your contam rates incredibly.

Otherwise, you did the best you can...

BTW, I have done open air innoculations for testing purposes and I usually get a 7 contams out of 12 where as the glovebox has never yeilded a contam.


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Offlinec0_hush
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Re: Another First Timer Stepping Out Into Cultivation [Re: Fraggin]
    #7607734 - 11/07/07 04:42 PM (16 years, 2 months ago)

Did you use 2 layers of foil while sterilization? I ask because that's what I've been wanting to do and not use the cap and lid that comes with the mason jars at all. I just want to use them at the end to sit the cakes on while they're in the terrarium.

My method will be using the bottom foil to inoculate the jars thru and use the top one (which is also sterile) as a 2nd layer, after inoculation.


--------------------


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OfflineSpooglywoogly
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Re: Another First Timer Stepping Out Into Cultivation [Re: c0_hush]
    #7607782 - 11/07/07 04:50 PM (16 years, 2 months ago)

Yep thats pretty much exactly what i did.

double layered the lower tier of foil and put a second thinner one above it. removed the first layer and inoculated through the double thickness one.


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OfflineSpooglywoogly
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Re: Another First Timer Stepping Out Into Cultivation [Re: Fraggin]
    #7607797 - 11/07/07 04:52 PM (16 years, 2 months ago)

interesting test about the open air contamination. i thought that the real difference was down to how clean u maintain the tools and level of physical sterility, not air flow.

next time i will definately make a glove box.


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