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OfflineRogerRabbitM
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Registered: 03/26/03
Posts: 42,214
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Re: fruit biopsy - iodine? [Re: mycocurious]
    #7609459 - 11/07/07 11:41 PM (16 years, 4 months ago)

You can clone any part of the fruit body. If using a scalpel as in the DVD, just tear the mushroom in half lengthwise to expose pure tissue. In general, take your tissue from the meatiest part of the mushroom to give you the best chance of getting a clean culture.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: fruit biopsy - iodine? [Re: RogerRabbit]
    #7609476 - 11/07/07 11:45 PM (16 years, 4 months ago)

so that sorta defeats this thread. if we're tearing them to get a nice meaty sample of pure tissue then there is no need for cleaners right? like you stated earlier.


even doing this with a syringe this would be the best way of doing this no?

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OfflineRogerRabbitM
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Re: fruit biopsy - iodine? [Re: blood4blood]
    #7610076 - 11/08/07 07:26 AM (16 years, 4 months ago)

You still need to clean it, especially if using a flow hood. The air from the flowhood blows across the fruit, knocking contaminants from the surface into the air, and then they swirl into your petri dish. In addition, they stick to your gloves when you handle the fruit, and that's another vector of contamination that is eliminated by disinfecting the exterior first.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Offlinespock1
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Registered: 04/14/07
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Re: fruit biopsy - iodine? *DELETED* [Re: RogerRabbit]
    #7612210 - 11/08/07 05:57 PM (16 years, 4 months ago)

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Invisibleblood4blood
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Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: fruit biopsy - iodine? [Re: spock1]
    #7612385 - 11/08/07 07:06 PM (16 years, 4 months ago)

why dont you just use a scalpel?

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