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Offlinedismantled02
Keep it Rockin'
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Registered: 10/28/07
Posts: 45
Loc: New England
Last seen: 15 years, 7 months
Question on amount of CC per jar
    #7600399 - 11/05/07 09:21 PM (16 years, 2 months ago)

How many cc's should I use per 1 pint jar for spores?


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InvisibleGlacier Creek
The Chef
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Registered: 09/23/07
Posts: 384
Loc: PNW
Re: Question on amount of CC per jar [Re: dismantled02]
    #7600431 - 11/05/07 09:26 PM (16 years, 2 months ago)

If you shake it up really well, you shouldnt need much at all. I wouldnt go any more than 1 cc per jar, but you can get away with less than that.


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Google "Earthly Info" to find my mushroom recipes. #1 baby.. yeah...

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Offlinexaxphaanes
Mycologist
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Registered: 08/08/05
Posts: 2,988
Last seen: 1 year, 5 months
Re: Question on amount of CC per jar [Re: Glacier Creek]
    #7600752 - 11/05/07 10:31 PM (16 years, 2 months ago)

I have done 2 drops with great success lol but that is really pushin it use about .5 cc per jar


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Offlinedismantled02
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Registered: 10/28/07
Posts: 45
Loc: New England
Last seen: 15 years, 7 months
Re: Question on amount of CC per jar [Re: xaxphaanes]
    #7601515 - 11/06/07 07:45 AM (16 years, 2 months ago)

Thanks guys.


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Invisiblemycocurious
Mike O. Kuerias
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Registered: 02/09/07
Posts: 1,265
Re: Question on amount of CC per jar [Re: dismantled02]
    #7601590 - 11/06/07 08:18 AM (16 years, 2 months ago)

Typically I aim for 1 CC per pint jar and 2 CC's per quart jar (when doing grains). This is split up equally between four inoculation ports/injection-points equally spaced along the edge glass inside the jars...

**Remember** - For PF style jars it's important to push the needle down past the dry vermiculite barrier before injection your spore solution into the substrate. And flame sterilize the needle until it's red-hot between each injection...the first few pico-liters of solution that flows through the hot tip will cool it down so there's no need to wait for it to cool.


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:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

- How Myco-Curious Prepares Coir & Compost Substrates
- How Myco-Curious Builds A Bulk Humidifier
- How Myco-Curious Builds An Automated Greenhouse
------------------------------------
figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.


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Invisibledumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
Re: Question on amount of CC per jar [Re: mycocurious]
    #7601780 - 11/06/07 09:29 AM (16 years, 2 months ago)

i've heard of people doing half cc's just make sure you get a spore in there....if you do that and shake good you can pull it off


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Invisiblemycocurious
Mike O. Kuerias
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Registered: 02/09/07
Posts: 1,265
Re: Question on amount of CC per jar [Re: dumbfounded1600]
    #7601817 - 11/06/07 09:42 AM (16 years, 2 months ago)

Quote:

dumbfounded1600 said:
...if you do that and shake good you can pull it off




Never shake your grains (can't really shake a pf-styled jar) after inoculation as it's very counter-productive. You want each one of those injections of spore solution to rest right where they were deposited so they can germinate and make their first reproductive connections with other compatible spores.


--------------------
:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

- How Myco-Curious Prepares Coir & Compost Substrates
- How Myco-Curious Builds A Bulk Humidifier
- How Myco-Curious Builds An Automated Greenhouse
------------------------------------
figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.


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