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Spooky6075
Stranger



Registered: 01/09/07
Posts: 135
Last seen: 3 years, 8 months
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syringe shelf life
#7600130 - 11/05/07 08:20 PM (16 years, 2 months ago) |
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I have had 4 spore syringes in my fridge for a month now im gonna use them tommarrow do you think there still good? and do i need to let it get room temp before i use it? thanks
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BUDDHA_702
Master Mycologist In Training



Registered: 02/17/07
Posts: 1,296
Loc: Some Country
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Re: syringe shelf life [Re: Spooky6075]
#7600160 - 11/05/07 08:25 PM (16 years, 2 months ago) |
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Probably, I would use a little more than normal just to help out a bit.
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Chi Ro
Jive Ass Turkey


Registered: 09/22/07
Posts: 211
Loc: Right hurr!
Last seen: 12 years, 5 months
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Re: syringe shelf life [Re: BUDDHA_702]
#7600184 - 11/05/07 08:30 PM (16 years, 2 months ago) |
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My Golden Teacher syringes were in my fridge for four months before use, and they worked just fine.
I say you'll be fine.
--------------------
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KaptKid
Spaced Pirate



Registered: 12/11/03
Posts: 6,252
Loc: Bright Side of the Sun
Last seen: 3 years, 11 months
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Re: syringe shelf life [Re: Spooky6075]
#7600419 - 11/05/07 09:24 PM (16 years, 2 months ago) |
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I had two stored in a desk draw for two years and they still work.
-------------------- Child of the 60's, Tripping ever since.
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mycocurious
Mike O. Kuerias



Registered: 02/09/07
Posts: 1,265
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Re: syringe shelf life [Re: KaptKid]
#7601693 - 11/06/07 08:56 AM (16 years, 2 months ago) |
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Quote:
KaptKid said: I had two stored in a desk draw for two years and they still work.
+1 I've had spore syringes stored at room temperatures for great lengths of time - although nowhere near two-years - that were still viable once used.
Keep in mind that partially used syringes will often get a small amount of nutritious content in them during their previous inoculation that will cause the spores to partially germinate. This is not the same as a liquid culture and it will dramatically reduce the syringe's lifespan as the partially germinated mycelium will weaken as it quickly runs out of nutrients. Even still, at room temperatures, I've had such partially used syringes remain viable for well over six months without issue, although they were, admittedly, poor performers when fruited because of their increased senility (old-age).
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Don't mistake my tone for a "matter-of-fact" attitude. I'm just presenting what I believe to be correct, until I'm corrected... - How Myco-Curious Prepares Coir & Compost Substrates - How Myco-Curious Builds A Bulk Humidifier - How Myco-Curious Builds An Automated Greenhouse ------------------------------------ figgusfiddus said: Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.
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Roadkill
Retired Shroomery Mod



Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: syringe shelf life [Re: Spooky6075]
#7601743 - 11/06/07 09:19 AM (16 years, 2 months ago) |
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Quote:
Spooky6075 said:
I have had 4 spore syringes in my fridge for a month now im gonna use them tommarrow do you think there still good? and do i need to let it get room temp before i use it? thanks
I have some spore syringes that have been in my frig for over 6 years now...
and they are still viable.
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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QDP843
Entrepreneur


Registered: 03/05/07
Posts: 295
Loc: SOUTHERN
Last seen: 15 years, 3 months
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Re: syringe shelf life [Re: Roadkill]
#7601760 - 11/06/07 09:25 AM (16 years, 2 months ago) |
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i had some sitting in a drawer room temp for 6 months and they worked fine
now, someone tell me, this
i have had 6 pint jars unused wrapped in plastic sitting in fridge for 3 weeks, will they be good to use still?
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Spooky6075
Stranger



Registered: 01/09/07
Posts: 135
Last seen: 3 years, 8 months
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Re: syringe shelf life [Re: Roadkill]
#7601765 - 11/06/07 09:25 AM (16 years, 2 months ago) |
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awsome but noone has told me if i should let them get room temp before i use them or inject them cold?
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QDP843
Entrepreneur


Registered: 03/05/07
Posts: 295
Loc: SOUTHERN
Last seen: 15 years, 3 months
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Re: syringe shelf life [Re: Spooky6075]
#7601766 - 11/06/07 09:26 AM (16 years, 2 months ago) |
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that i dont know
i mean, might as well just let them get room temp
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mycocurious
Mike O. Kuerias



Registered: 02/09/07
Posts: 1,265
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Re: syringe shelf life [Re: QDP843]
#7601806 - 11/06/07 09:38 AM (16 years, 2 months ago) |
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it's a moot point, either way at some point the spores are going to reach room temperature and begin germinating in the nutrient rich spawn medium you created in the jar.
I don't refrigerate them because, in my humble opinion, it's a risky business. The first time someone throws something in from the freezer (e.g. a turkey) to thaw in the fridge you risk the syringes freezing as the turkey thaws. That's simple thermodynamics. The refrigerator isn't warming the turkey, the turkey is sucking all the available heat (energy) out of every other item in the refrigerator until they are all the same temperature.
The same is true when you load up your refrigerator with new items from the grocery store... they cool off quickly because all of the existing, colder items in the unit are trying to suck the energy out of warmer items until they're all the same temperature. Throw a couple 12-packs of warm soda/beer into the fridge and come back in 30 minutes and you'll find that your milk is a good deal warmer than it was before you added the new items.
--- Maybe I'm just a little paranoid about that stuff, lol...
--------------------
Don't mistake my tone for a "matter-of-fact" attitude. I'm just presenting what I believe to be correct, until I'm corrected... - How Myco-Curious Prepares Coir & Compost Substrates - How Myco-Curious Builds A Bulk Humidifier - How Myco-Curious Builds An Automated Greenhouse ------------------------------------ figgusfiddus said: Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.
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