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OfflineKCizzle
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Got Nutrient Agar by accident. Still ok to use?
    #7599307 - 11/05/07 05:07 PM (16 years, 2 months ago)

I wasn't paying attention when purchasing. Is Nutrient Agar OK to use the same way Potato Dextrose Agar is used?


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InvisibleThePyschonaut52
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Re: Got Nutrient Agar by accident. Still ok to use? [Re: KCizzle]
    #7599320 - 11/05/07 05:12 PM (16 years, 2 months ago)

I love BC...

I would think it should still work. Give it a try!


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"In god we trust..."


-I guess we're screwed.


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OfflineKCizzle
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Re: Got Nutrient Agar by accident. Still ok to use? [Re: ThePyschonaut52]
    #7599516 - 11/05/07 06:04 PM (16 years, 2 months ago)

Yeah i'm gonna give it a try regardless. That'd be cool if it worked out better than PDA


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Invisibleorchidfanatic
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Re: Got Nutrient Agar by accident. Still ok to use? [Re: KCizzle]
    #7599583 - 11/05/07 06:19 PM (16 years, 2 months ago)

you can grow orchid seeds on it !!!!


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Invisiblemycocurious
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Re: Got Nutrient Agar by accident. Still ok to use? [Re: KCizzle]
    #7601728 - 11/06/07 09:12 AM (16 years, 2 months ago)

Quote:

KCizzle said:
I wasn't paying attention when purchasing. Is Nutrient Agar OK to use the same way Potato Dextrose Agar is used?




Is it a trick question? Malt-Extract Agar (MEA), Potato-Dextrose Agar (PDA) and Blood Agar (usually sheep's blood) are all forms of nutrient agar...

I was under the impression that a nutrient agar was simply an agar-agar solution that was combined with some form of nutrient selected for the type of growth you're attempting to encourage...

Now, as for what type of nutrient you have in your "Nutrient Agar", I have no idea...


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:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

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Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.


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OfflineRogerRabbitM
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Re: Got Nutrient Agar by accident. Still ok to use? [Re: mycocurious]
    #7601796 - 11/06/07 09:34 AM (16 years, 2 months ago)

Normally, the term nutrient agar is used to refer to agar that has blood or other ingredients to tune it specifically to bacteria, which is what we are trying to tune against. I recommend MEA.
RR


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OfflineKCizzle
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Re: Got Nutrient Agar by accident. Still ok to use? [Re: RogerRabbit]
    #7601968 - 11/06/07 10:33 AM (16 years, 2 months ago)

Aaah gotcha. Ok cool well already ordered the PDA but I will post my test results for the Nutrient Agar. Yeah its called Nutrient Agar right on the bottle. Got it from OnlineScienceMall by accident. D'oh


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OfflineKCizzle
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Re: Got Nutrient Agar by accident. Still ok to use? [Re: KCizzle]
    #7607438 - 11/07/07 03:32 PM (16 years, 2 months ago)

COMPLETE disaster. Stinky stinky bacteria in just a couple days. Like my friend said above it looks like this nutrient agar it geared more towards bacteria cultures. WHEW I dont know how the scientist that work with this stuff actually do. Smells like death took a shit.


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Invisiblefastfred
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Re: Got Nutrient Agar by accident. Still ok to use? [Re: KCizzle]
    #7608301 - 11/07/07 06:43 PM (16 years, 2 months ago)

Nutrient agar is actually quite specificaly defined...

http://www.cfsan.fda.gov/~ebam/m112.html

Nutrient Agar FDA M112

Beef extract 3 g
Peptone 5 g
Agar 15 g
Distilled water 1 liter

Heat to boiling to dissolve ingredients. Dispense into tubes or flasks. Autoclave 15 min at 121°C. Final pH, 6.8 ± 0.2. If used as base for blood agar, add 8 g NaCl to prevent hemolysis of blood cells.

Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.


Oh and nutrient agar probably would have worked had you not contaminated it. Growth is pretty slow and shitty compared to PDA or MEA if I remember right. But even LB agar will grow myc slowly.


-FF


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Offlinezathan
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Re: Got Nutrient Agar by accident. Still ok to use? [Re: fastfred]
    #7608805 - 11/07/07 08:48 PM (16 years, 2 months ago)

Yup, I can also vouch that myc growth on blood agar is pretty poor.


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