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Offlineayuds5872
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Registered: 10/31/07
Posts: 18
Last seen: 16 years, 1 month
Species colination time
    #7598021 - 11/05/07 10:59 AM (16 years, 2 months ago)

i inoculated some creeper and brazillian about a week ago into the center of some brown rice cakes and i only have one hole. how long should it take before i should start seeing growth?


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OfflineLeonidas25
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Registered: 06/16/07
Posts: 85
Last seen: 15 years, 11 months
Re: Species colination time [Re: ayuds5872]
    #7598030 - 11/05/07 11:03 AM (16 years, 2 months ago)

I would think relatively soon youll see something


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OfflineMarlboroMan
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Re: Species colination time [Re: Leonidas25]
    #7598065 - 11/05/07 11:14 AM (16 years, 2 months ago)

Brazilians are one of (if not THE) fastest colonizing cubensis strain out there. You should see growth soon. You should really try at least two holes, or even four for faster colonization. Injecting it straight into the middle will make it colonize the middle first, which os probably why you haven't seen much if any growth. Just make sure you split up the amount you are going to inject (usually around 1cc per jar) evenly into each hole and try to release it against the inside wall of the jar. Also, make sure to use some micropore tape or foil to cover the holes. Good luck to ya! :rockon:


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I'll make it to the moon if I have to crawl..


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Invisiblemycocurious
Mike O. Kuerias
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Registered: 02/09/07
Posts: 1,265
Re: Species colination time [Re: MarlboroMan]
    #7598366 - 11/05/07 12:41 PM (16 years, 2 months ago)

Generally speaking, we try to aim for four 3/8" or small injection ports through the lids of our spawn jars when working with grains or cakes. Remember these holes are not only designed to provide a way "in" for inoculations but also a means for gas-exchange for the newly colonizing mycelium you've begun cultivating. Another reason we use four injection ports is because it's often a lot easier to observe the colonization process if you inject your inoculate / spore-solution along the sides of the glass rather than down the center. Inoculating in the center may take a couple weeks before you can visually observe any signs of colonization/contamination.

Don't over do it though because too many or too large of an opening becomes counter-productive because it will allow too much internal moisture to be lost from evaporation.

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You don't need any foil or micro-pore tape over the top of a PF-Styled jar because the dry layer of vermiculite on top is your contaminate barrier and anything else only further restricts the gas-exchange.


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:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

- How Myco-Curious Prepares Coir & Compost Substrates
- How Myco-Curious Builds A Bulk Humidifier
- How Myco-Curious Builds An Automated Greenhouse
------------------------------------
figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.


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Offlineayuds5872
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Registered: 10/31/07
Posts: 18
Last seen: 16 years, 1 month
Re: Species colination time [Re: mycocurious]
    #7601586 - 11/06/07 08:17 AM (16 years, 2 months ago)

alright thanks, but i did check the jars again today and it looks like a darker spot is forming it doesn't lok like mold it just loks like the verm is turning dark or even black


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OfflineSkeeblix
Dave Thomas
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Registered: 01/28/07
Posts: 1,745
Loc: Wendy's
Last seen: 8 years, 3 months
Re: Species colination time [Re: ayuds5872]
    #7601613 - 11/06/07 08:26 AM (16 years, 2 months ago)

If stuff is turning any color other white (which can still be a contam in some cases) it's more than likely contaminated.

If it turns out to be black mold after a few more days, don't open it. Either throw the jar away, or PC it for 30 or 45 minutes, take it outside, and empty it out while you hold a trash bag around the mouth of the jar. Set your jar and lid down, tie the bag up, and then put it in another bag.

It's a lot easier to just throw them out, and you take less risk of exposing yourself to black mold, which can be extremely hazardous to you health.


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Premedman1 said:
:lol: I just shat my pants.


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Offlineayuds5872
Stranger

Registered: 10/31/07
Posts: 18
Last seen: 16 years, 1 month
Re: Species colination time [Re: Skeeblix]
    #7601955 - 11/06/07 10:30 AM (16 years, 2 months ago)

so i inoculated 7 days ago with brazillian and i have yet to see any growth in the jars with one hole, is that normal?


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Offlineayuds5872
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Registered: 10/31/07
Posts: 18
Last seen: 16 years, 1 month
Re: Species colination time [Re: ayuds5872]
    #7602040 - 11/06/07 10:51 AM (16 years, 2 months ago)
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heres a pic of the darker stuff i just wanna make sure it's not contaminated. if it is i'll just start over again


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