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OfflineWeedyWonka
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Quick question
    #7597890 - 11/05/07 10:06 AM (16 years, 2 months ago)

I sterilized a few jars the other day and since then they have been sealed off and in a sterile glove box. Here is my question.

If the jars show no sign of growth in them after 3 days does that mean they are contam free? They are still very as clear as it was after I stereilized it.

If they are contam free does that mean that as long as I am sterile when I put in the spores I will have a good LC.


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OfflineLeonidas25
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Re: Quick question [Re: WeedyWonka]
    #7597907 - 11/05/07 10:11 AM (16 years, 2 months ago)

You want to inoculate your jars as soon as they have cooled to room temp


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InvisibleSlimz
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Re: Quick question [Re: Leonidas25]
    #7597955 - 11/05/07 10:33 AM (16 years, 2 months ago)

actually this sounds like a neat idea.. 3 days is not nearly long enough.. But if i had a few jars that i sterilized and left alone, sealed, for a few weeks without any signs of contam, WITH injection ports, i would feel relatively safe that they were sterile and ready to use...


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Lazy Drywall Tek (no powdery mess)

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Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

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Invisiblejeetered
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Re: Quick question [Re: Slimz]
    #7597989 - 11/05/07 10:47 AM (16 years, 2 months ago)

wouldn't the waiting throw off water content. Just a thought, i could be absolutely wrong.


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OfflineLeonidas25
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Re: Quick question [Re: jeetered]
    #7597999 - 11/05/07 10:49 AM (16 years, 2 months ago)

I'd think so


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InvisibleSlimz
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Re: Quick question [Re: Leonidas25]
    #7598012 - 11/05/07 10:54 AM (16 years, 2 months ago)

not if they are sealed in a mason jar..


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Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


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OfflineLeonidas25
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Re: Quick question [Re: Slimz]
    #7598024 - 11/05/07 11:01 AM (16 years, 2 months ago)

Yea...but why would you PC them sealed? Then you would have to take the entire lid off to inoculate, not to mention the vacum sucking air in when you do actually open it could cause contam...I PC with a couple holes in the lid and a synthetic filter disc on top...that way when I inoculate in front of my hood I only need to take the outer part of the lid and the filter disc off to expose the small holes in the lid, in goes the needle, on goes the filter and lid...


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OfflineRogerRabbitM
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Re: Quick question [Re: Leonidas25]
    #7598084 - 11/05/07 11:21 AM (16 years, 2 months ago)

A couple of points that would help new growers get proper answers:

Don't ask questions about 'jars'. Jars are plain glass and nothing grows on glass. It's important to say what's INside the jars.

In addition, it's best to follow established procedures for your first few grows. Let those who have grown before show you how by reading the established and respected teks. Once you have a few grows under your belt, experiment away because it's how we learn new things.

There is no need to let 'jars' sit after sterilization. If you followed proper sterilization procedure, the contents of your jars are sterile(right now), so don't second guess your pressure cooker. Inoculate as soon as they've cooled, or at least within 24 hours. No sterilization is ever complete, but rather it only gives a window for the mushroom mycelium to get a foothold before the surviving contaminants do. Waiting to inoculate decreases your odds of success. Good luck.
RR


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InvisibleSlimz
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Re: Quick question [Re: RogerRabbit]
    #7598104 - 11/05/07 11:26 AM (16 years, 2 months ago)

ok.. but what if you sterilized say 50xBRF/Verm jars one day and sealed em all up and put them away for later.. if they were hot when you put the lids on, then you have essentially "Canned" them and they should stay sterile for a long time.. You would have to clean them with bleach and then open them in a glove-box to use them.. but it would work...


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


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OfflineLeonidas25
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Re: Quick question [Re: Slimz]
    #7598122 - 11/05/07 11:31 AM (16 years, 2 months ago)

No doubt about that, if you needed to wait, it would work:thumbup:


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OfflineWeedyWonka
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Re: Quick question [Re: Slimz]
    #7598130 - 11/05/07 11:32 AM (16 years, 2 months ago)

Thats exactly the question I was trying to convey slimz just worded it a lit better.

Also the reason I used the term "Jar" was because I wasn't talking specifically one kind of substrate. (personally I am using an LC) But in theory if I sterilized a a batch of jars and ended up makeing more than I needed could I set them aside for later use?


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InvisibleSlimz
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Re: Quick question [Re: WeedyWonka]
    #7598153 - 11/05/07 11:38 AM (16 years, 2 months ago)

Yes.. Thats what i was saying.. it would work great.. its called canning.. you would be canning substrate.. If i can eat canned green beans then myc can eat canned substrate


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


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OfflineMarlboroMan
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Re: Quick question [Re: WeedyWonka]
    #7598166 - 11/05/07 11:41 AM (16 years, 2 months ago)

I had to wait about a week after sterilizing 9 of my jars. I simply put them in a box i had sprayed down good with disinfectant, then put that box in my TiT which had also been properly sterilized. About twice a day I would spray the area my TiT was in with disinfectant. I knocked them up about a week ago, and am having fantastic growth. If you sterilized, then there shouldn't be much to worry about, as long as you keep the environment they are being held in very clean as well. I hope this helps any. :rockon:


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OfflineLeonidas25
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Re: Quick question [Re: WeedyWonka]
    #7598175 - 11/05/07 11:45 AM (16 years, 2 months ago)

Just make sure theyre completely sealed (canned)...and be careful when you open to inoculate


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OfflineRogerRabbitM
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Re: Quick question [Re: MarlboroMan]
    #7598198 - 11/05/07 11:53 AM (16 years, 2 months ago)

Quote:

If i can eat canned green beans then myc can eat canned substrate





If you used as much salt as a preservative in your grain jars as they use in the green beans, nothing would grow on them, therefore you're not canning. Sterilization is never complete, and is time sensitive. Sterilized media should be used as soon as possible. Every day you wait, decreases the chances of success. Waiting a week or two almost always works out ok. Longer than that, and you're playing roulette.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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InvisibleSlimz
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Re: Quick question [Re: RogerRabbit]
    #7598220 - 11/05/07 12:02 PM (16 years, 2 months ago)

WOW.. here is a FIRST.. im gonna have to go ahead and disagree with you there roger

A widely-used method for heat sterilization is the autoclave(pressure cooker). Autoclaves commonly use steam heated to 121 °C (250 °F), at 103 kPa (15 psi) above atmospheric pressure. Solid surfaces are effectively sterilized when heated this temperature for at least 15 minutes or to 134 °C for a minimum of 3 minutes. However, liquids and instruments packed in layers of cloth require a much longer time to reach a sterilizing temperature. After sterilization, autoclaved liquids must be cooled slowly to avoid boiling over when the pressure is released.

Proper autoclave treatment will inactivate all fungi, bacteria, viruses and also bacterial spores, which can be quite resistant. It will not necessarily eliminate all prions.

For prion elimination, various recommendations state 121–132 °C (270 °F) for 60 minutes or 134 °C (273 °F) for at least 18 minutes.



The above is from Wikipedia.

The fact is that if you preasure cook substrate at 300f at 15 psi for 60 min, its sterile...


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


Edited by Slimz (11/05/07 12:42 PM)


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