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Fungus_Farming
Botanist

Registered: 08/24/02
Posts: 87
Loc: Next Door
Last seen: 5 years, 7 days
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MASSIVE CONTAMINATION PROBLEMS!!
#7594812 - 11/04/07 02:35 PM (16 years, 2 months ago) |
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I am having super problems. I have contamed 30 outta my last 35 casings(WBS spawn on verm/peat 50/50 in 11x9cake tins). I need some advice. I am doing 5at a time. only one is making it out contam-free. I was doing casings in a bathroom. I would spray the lysol of death for good couple minutes. then do 2 or 3,spray again and finish last couple. I now have access to a entire bedroom. I wanna make a little sterile enviroment,but need advice. Anyone have a super cheap tek on little sterile Bubble?(no glove box ideas plz. I want bigger! for bulk luv) =] Or anything that can make this work. I have lotsa colonized jars but hesitant to case,So quicker the better. Thanks ahead of time for any advice!
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Bikerfool
Your Local Edgelord



Registered: 11/21/05
Posts: 1,577
Last seen: 5 months, 13 days
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Re: MASSIVE CONTAMINATION PROBLEMS!! [Re: Fungus_Farming]
#7594842 - 11/04/07 02:43 PM (16 years, 2 months ago) |
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Bathrooms are gross, I wouldn't work in one. Lysol can cause mushroom mutations, I never use it. Are you balancing the peat pH? You need to add hydrated lime or calcium carbonate to your peat or else it will be a magnet for trich due to the low pH. What is your pasturization technique?
-------------------- Just an angsty teen contributing to the pubs decline with contentless posts.
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HappyHardcore
Shroomery Jester



Registered: 11/06/05
Posts: 259
Loc: All Over The World
Last seen: 10 years, 10 months
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Re: MASSIVE CONTAMINATION PROBLEMS!! [Re: Bikerfool]
#7594940 - 11/04/07 03:10 PM (16 years, 2 months ago) |
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If you're getting that much contamination, it could also be that your place has a very high spore count of contams and such. Have you thought about researching how to bleach clean everything? I've never needed to do this, nor had the problem of that much contamination so I can only point you in a direction. A thorough clean (with bleach/other strong cleaning stuff) will help atleast if your problem or even part of your problem is spore count in the area. Goodluck on getting that fixed, that has to be really frustrating!
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Fungus_Farming
Botanist

Registered: 08/24/02
Posts: 87
Loc: Next Door
Last seen: 5 years, 7 days
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Re: MASSIVE CONTAMINATION PROBLEMS!! [Re: HappyHardcore]
#7599610 - 11/05/07 06:26 PM (16 years, 2 months ago) |
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well i wanna build a little sterile room outta pvc and plastic wrap. i just need some advice on the air circulation. do i just zip myself in there and make no ventilation or do i make some kinda hepa filter? somone has to have some pictures or teks on this type of thing. any help would be greatly apretiated
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: MASSIVE CONTAMINATION PROBLEMS!! [Re: Fungus_Farming]
#7599835 - 11/05/07 07:14 PM (16 years, 2 months ago) |
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You could spawn a bulk substrate, or case a tray of grains in a sewer plant and succeed. That isn't where your problem is. If your bulk projects are contaminating prior to first flush, the problem is in your spawn. You need to pay more attention to correct inoculation procedures, and have proper filters on your jars. That means using a good glovebox even if that isn't what you wanted to hear.
In my DVD, I demonstrate making a laundry basket of straw, and a straw log on my nasty kitchen floor, and kitchens are the worst possible place to do sterile work. The reason it succeeds is because once the spawn is colonized, it is resistant to contamination, and a properly pasteurized bulk substrate or casing material will not contaminate for two weeks, no matter what lands on it.
Fix your spawn generation procedure and your other problems will go away, provided you're not sterilizing the casing or bulk material. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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mycocurious
Mike O. Kuerias



Registered: 02/09/07
Posts: 1,265
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Re: MASSIVE CONTAMINATION PROBLEMS!! [Re: RogerRabbit]
#7600041 - 11/05/07 08:01 PM (16 years, 2 months ago) |
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+1 on it not being the location. As horrible as it may sound, the only time I pull out my makeshift glovebox is when working with agar...everything else is done open air in my hall-bathroom. Well, my grain-to-grains are done inside a new, clean clear garbage bag because they're sterilize by way of their manufacturing process and it's just easier to manipulate the jars in the bags than within my glovebox. Still the only time I've had a contaminated jar was when I stabbed the caulking with a needle and inoculated anyways. Black pin mold in a week...
A sound, repeatable cleaning process along with a sterile inoculation technique is the key requirement...the most important of which - in my humble opinion - is *YOUR* grooming process... Shower, brushing your teeth, shaving, pulling your hair back into a hat, wear freshly laundered -ideally still warm from the tumbler - long-sleeve shirts and pants (tuck the pants into your socks) along with tyvek arm-sleeves (they're cheap, get some) over the shirt and over the gloves.
Those would be the *minimum* requirements, in my humble opinion...
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Don't mistake my tone for a "matter-of-fact" attitude. I'm just presenting what I believe to be correct, until I'm corrected... - How Myco-Curious Prepares Coir & Compost Substrates - How Myco-Curious Builds A Bulk Humidifier - How Myco-Curious Builds An Automated Greenhouse ------------------------------------ figgusfiddus said: Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.
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Fungus_Farming
Botanist

Registered: 08/24/02
Posts: 87
Loc: Next Door
Last seen: 5 years, 7 days
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Re: MASSIVE CONTAMINATION PROBLEMS!! [Re: mycocurious]
#7603103 - 11/06/07 03:05 PM (16 years, 2 months ago) |
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Alrite, i have no problem with jars. i have contamed mayb 3 out of the last 100. i do however sterilize my casing material. perhaps that is my problem. i microwave my casing material. i will try pasturizing my 50/50 b4 i spend lotsa money on this sterile box. hopefully that solves all my problems. Thanks RR
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milkman
DeliveringWorldWide



Registered: 07/04/07
Posts: 2,108
Loc: tha FLA
Last seen: 2 months, 29 days
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Re: MASSIVE CONTAMINATION PROBLEMS!! [Re: Fungus_Farming]
#7603133 - 11/06/07 03:10 PM (16 years, 2 months ago) |
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97 pecent nice i have had 1 outa 16 so im notoff so godbut after my firstflush of mgmc cased substrate i had contams out the ass ive cleaned my procedures up since
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