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Offlinem.v2
Apple-Core
Male


Registered: 08/22/07
Posts: 307
Last seen: 3 years, 7 months
Keeping syringe sterile
    #7589434 - 11/02/07 08:52 PM (16 years, 2 months ago)

Hey guys,
I've got a 20ml plunger and an 18 gauge needle, problem is they're in separate packets.
I'm approaching my first inoculation and I was wondering, if I just attach the needle to the plunger in my glovebox will that be sterile enough?

Or is there some extra precaution I should go to?
Cheers.


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InvisibleBUDDHA_702
Master Mycologist In Training
Male


Registered: 02/17/07
Posts: 1,296
Loc: Some Country
Re: Keeping syringe sterile [Re: m.v2]
    #7589459 - 11/02/07 09:00 PM (16 years, 2 months ago)

That should be good enough, But I like to keep the needle in a alcohol soaked cotton roll.


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OfflineManianFHS
living in perverty
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Registered: 07/06/04
Posts: 14,741
Last seen: 1 day, 5 hours
Re: Keeping syringe sterile [Re: BUDDHA_702]
    #7589470 - 11/02/07 09:04 PM (16 years, 2 months ago)

ya just soak everything in alcohol, and spray anti-bacterial shit everywhere. If you're gonna wait a month to see results from 5 minutes of work, make sure the 5 min of work is done with extra care

and, when injecting the needle into the top of the jar, do it through a couple pads of alcohol paper placed atop the can as well, or a cotton ball w/ alcohol or something.


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notapillow said: "you are going about this endeavor all wrong. clear your mind of useless fear and concern. buy the ticket, take the ride, and all that.... "

ChrisWho said: "It's all about the journey, not the destination."


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Offlinem.v2
Apple-Core
Male


Registered: 08/22/07
Posts: 307
Last seen: 3 years, 7 months
Re: Keeping syringe sterile [Re: ManianFH]
    #7589500 - 11/02/07 09:14 PM (16 years, 2 months ago)

Okay, sweet
Thanks for the replies guys.


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Offlineveda_sticks
Cultivator
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Folding@home Statistics
Registered: 07/29/07
Posts: 14,191
Loc: UK Flag
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Re: Keeping syringe sterile [Re: m.v2]
    #7589569 - 11/02/07 09:39 PM (16 years, 2 months ago)

I have read somewhere about using an alcohol soaked sponge as a needle guard, seems like a really good idea, keeps the needle clean between inocs. Flame the needle sterile first then use a soaked peice of sponge and cover the needle with it, seem slike a great method. Im going to be using that with my next inoculations in conjuction with a glovebox.

But yeah, when u put the needle onto the syringe, in the glove box, after you have used it, flame the needle red hot, wipe with alcohol soaked pad then put the pastic needle cap on.

Leave in the frigde, next time u use, sterile procedure in the glove box, flame needle wipe with alchole and inoc away.

Its all in the teks


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
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Franks Spawning To Bulk - Monotub
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Foo Mans No Soak WBS Prep Tek


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Offlinekatfish
Nooblit
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Registered: 07/22/06
Posts: 592
Loc: Louisiana
Last seen: 15 years, 11 months
Re: Keeping syringe sterile [Re: veda_sticks]
    #7589992 - 11/03/07 12:48 AM (16 years, 2 months ago)

i wipe or dip in alchol and then light the needle on fire and wait till it burns out, or just use a lighter until you can see that it is red hot, but let it cool for a long enough time because you will kill the spores because of the heat.

katfish


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08/2/07 Inoculated mycobag with Brazilian, 3cc
08/08/07 First mycelium show
09/03/07 Brazilian still colonizing, Three Mycobags inoculated with Koi Somui
10/11/07 2 Pint PESA 100% colonized. 1 pint Amazon 90% colonized
10/12/07 Storage bin perite FC constructed
10/28/07 Havested my First ever flush, of PESA!


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