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Offlinearmyabe
Mimic
Male


Registered: 09/13/07
Posts: 240
Loc: Washington state
Last seen: 16 years, 23 days
I want to do LC but...
    #7583584 - 11/01/07 10:58 AM (16 years, 2 months ago)

But i haven't gotten anything concrete. all the LC teks talk about a filter but doun't explain the best kind, where to put it, how big it needs to be. can someone post a picture of there LC jar or explain this to me. a couple of the LC's talked about no gas exchange????


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What if I am wrong, Ive got a condition, I get confused sometimes, what if I have imaged all of this, what if have finally turned into what they've always said I was gonna turn into.... a maniac...... a psycho killer.....Marv Sin City


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InvisibleCaptain Cubensis
Bleeding HeartLiberal


Registered: 09/18/07
Posts: 648
Re: I want to do LC but... [Re: armyabe]
    #7583822 - 11/01/07 12:07 PM (16 years, 2 months ago)

All you need is a 1/8-1/4 inch hole covered with micro-pore tape.

Be sure you don't over PC, the browner the downer, as sugars caramelize pretty easily.

My foaf take the PC up to 15 psi then turns off the heat, seems to work well.


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Offlinearmyabe
Mimic
Male


Registered: 09/13/07
Posts: 240
Loc: Washington state
Last seen: 16 years, 23 days
Re: I want to do LC but... [Re: Captain Cubensis]
    #7600229 - 11/05/07 08:38 PM (16 years, 2 months ago)

what about if you don't have a pc yet? is micro spore better than tyvek? 1/2 pints best keep it lifted or part in the water. two everyones experiance is honey best?


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What if I am wrong, Ive got a condition, I get confused sometimes, what if I have imaged all of this, what if have finally turned into what they've always said I was gonna turn into.... a maniac...... a psycho killer.....Marv Sin City


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Offlineacommunistspy
the fun nazi,NOT to beconfused withthe fun-nazi


Folding@home Statistics
Registered: 08/13/07
Posts: 657
Loc: the garden state
Last seen: 13 years, 10 months
Re: I want to do LC but... *DELETED* [Re: armyabe]
    #7600280 - 11/05/07 08:51 PM (16 years, 2 months ago)

Post deleted by acommunistspy

Reason for deletion: this never happened



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Edited by acommunistspy (11/05/07 08:54 PM)


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Offlinearmyabe
Mimic
Male


Registered: 09/13/07
Posts: 240
Loc: Washington state
Last seen: 16 years, 23 days
Re: I want to do LC but... [Re: acommunistspy]
    #7601698 - 11/06/07 09:00 AM (16 years, 2 months ago)

where do i find all that stuff? magnetic things that is. quart jars? huh.


mmmm thanks


--------------------
What if I am wrong, Ive got a condition, I get confused sometimes, what if I have imaged all of this, what if have finally turned into what they've always said I was gonna turn into.... a maniac...... a psycho killer.....Marv Sin City


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Offlineacommunistspy
the fun nazi,NOT to beconfused withthe fun-nazi


Folding@home Statistics
Registered: 08/13/07
Posts: 657
Loc: the garden state
Last seen: 13 years, 10 months
Re: I want to do LC but... *DELETED* [Re: armyabe]
    #7601792 - 11/06/07 09:33 AM (16 years, 2 months ago)

Post deleted by acommunistspy

Reason for deletion: this never happened



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Offlinearmyabe
Mimic
Male


Registered: 09/13/07
Posts: 240
Loc: Washington state
Last seen: 16 years, 23 days
Re: I want to do LC but... [Re: acommunistspy]
    #7634490 - 11/14/07 11:08 AM (16 years, 2 months ago)

would someone post a pic of there LC lid please thanks


--------------------
What if I am wrong, Ive got a condition, I get confused sometimes, what if I have imaged all of this, what if have finally turned into what they've always said I was gonna turn into.... a maniac...... a psycho killer.....Marv Sin City


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OfflineCaribou_Lou
Stranger


Registered: 10/17/07
Posts: 2,510
Loc: Never Land
Last seen: 12 years, 10 months
Re: I want to do LC but... [Re: armyabe]
    #7635181 - 11/14/07 01:25 PM (16 years, 2 months ago)

Do the baby bottle LC tek


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Offlinebuggas
Stranger


Registered: 09/27/07
Posts: 145
Last seen: 15 years, 5 months
Re: I want to do LC but... [Re: Caribou_Lou]
    #7635743 - 11/14/07 03:33 PM (16 years, 2 months ago)

You can use whatever jars you want.

I poke/drill two holes in the lid, cover one with one piece of micropore tape, and the other hole, i cover on both sides with a dab of silicone.

The micropore is for gas exchange, the silicone is a "self healing injection port", basically the silicone will close back up after you take the needle out.

Do a 4% solution of honey and hot water. I put 1tsp to 100ml of water. Boil or PC as desired, just like PF cakes, and then let cool. Inject your spores into the silicone port, and then just wait.

It takes longer in cooler temps so if you can, put them somewhere where the temp is in the high 70's. Just like incubating. Instead of the myc growing on cakes, they're growing in water. Nothing special.

The real difference is that you want to shake the jars up a bit every so often so that the myc breaks up and will be easier to suck up into the syringe.

Remember, you're looking for boogers in your water, not pulp, sediment or spores, just whitish looking boogers. Sometimes they're on the bottom but you will know when it's right, it will look like myc from the cakes, but in water.

To find out how to do a lid, just search for "Self Healing Injection Ports" and you'll see. I just improvised and used only one for injection and the other for gas exchage.

Some people even put ~3in. long, polyfilled tubes coming out of the 2 holes so that they can shake up the jars and not worry about getting the tvyek, polyfill or micropore wet and wicking in contams. This is a good idea to me, and i think i'm going to try it next time i make an LC.


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There are no stupid questions, just stupid answers from stupid people.

Watch Zeitgeist and Tell Others


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InvisibleMojo
Stranger

Registered: 07/12/07
Posts: 1,676
Re: I want to do LC but... [Re: armyabe]
    #7635825 - 11/14/07 03:54 PM (16 years, 2 months ago)

Don’t make things harder than they need to be, its vary simple.

Two holes with a standard screw driver dab some standard silicone in and over one of the holes (top and bottom of the hole) and cover the other one with some micro-por tape.

Pics:





Use this link for malt/dextrose ratios, and technique. If you’re not going to use a pressure cooker than I recommend boiling the liquid culture like Agar says to in this tek.

http://www.shroomery.org/forums/showflat.php/Number/6673631#Post6673631


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Offlinerice_smuggler
Stranger
Male


Registered: 10/18/07
Posts: 28
Last seen: 16 years, 27 days
Re: I want to do LC but... [Re: Mojo]
    #7636154 - 11/14/07 05:10 PM (16 years, 2 months ago)

Here's my rendition of the baby bottle LC tek using honey (pic is prior to PC'ing):


I just flipped the nipple upside down, put some high temperature silicone in the nipple tip, drilled a hole on the side and plumbed a silicone tube (stuffed w/polyfill) for air exchange and sealed with more silicone, and finally dropped a marble inside to serve as an agitater.

Once the bottle is ready pour in some honey and water (4 ml/cc of honey + 96 ml/cc H20), pressure cook at 15 PSI for 15 to 20 minutes, allow bottles to cool inside the pressure cooker to room temperature then inoculate with your choice of spore/tissue. At the end of the day it doesn't have to look pretty as long as it works.


Edited by rice_smuggler (11/14/07 05:14 PM)


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