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InvisibleCaptain Cubensis
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Registered: 09/18/07
Posts: 648
When to pull the plug?
    #7583447 - 11/01/07 10:08 AM (16 years, 2 months ago)

How long would you wait before ditching a 50/50+ cased rye grain that does not seem to want to colonize the casing?


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InvisibleSlimz
.-~*´`*·~-experience-~*´`*·~-.
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Registered: 10/03/07
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Re: When to pull the plug? [Re: Captain Cubensis]
    #7583450 - 11/01/07 10:09 AM (16 years, 2 months ago)

untill it dies..


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InvisibleCaptain Cubensis
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Registered: 09/18/07
Posts: 648
Re: When to pull the plug? [Re: Slimz]
    #7583495 - 11/01/07 10:25 AM (16 years, 2 months ago)

Quote:

Slimz said:
until it dies..




But, if it hasn't colonized the casing, how would you know if it's dead?

It is a well known slow colonizer.


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InvisibleAlteredAgain
Visual Alchemist
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Registered: 04/27/06
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Re: When to pull the plug? [Re: Captain Cubensis]
    #7583498 - 11/01/07 10:27 AM (16 years, 2 months ago)

how long since you cased? wait until it's colonized.


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InvisibleCaptain Cubensis
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Registered: 09/18/07
Posts: 648
Re: When to pull the plug? [Re: AlteredAgain]
    #7583516 - 11/01/07 10:35 AM (16 years, 2 months ago)

Well, that's what I am asking really, is how long would you wait?


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InvisibleAlteredAgain
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Re: When to pull the plug? [Re: Captain Cubensis]
    #7583520 - 11/01/07 10:36 AM (16 years, 2 months ago)

10 days IME give or take.


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OfflineMotorCityMadman
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Re: When to pull the plug? [Re: AlteredAgain]
    #7583580 - 11/01/07 10:56 AM (16 years, 2 months ago)

I'd pitch it whenever it shows any sign of infection (trich, etc). Until then it's a question of tweaking environmental factors.

How thick is your casing layer? Is this your first try or have you had success before?


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Offlinearmyabe
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Registered: 09/13/07
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Loc: Washington state
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Re: When to pull the plug? [Re: MotorCityMadman]
    #7583588 - 11/01/07 11:00 AM (16 years, 2 months ago)

is this a casing or a pf jar?


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InvisibleCaptain Cubensis
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Registered: 09/18/07
Posts: 648
Re: When to pull the plug? [Re: armyabe]
    #7583592 - 11/01/07 11:02 AM (16 years, 2 months ago)

Rye berries, 2 inches thick, cased with about 1/4 inch Jiffy Mix, pHd up to about 7.9.

Jars were cased 16 days ago, no sign of myc!


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Offlinearmyabe
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Re: When to pull the plug? [Re: Captain Cubensis]
    #7583637 - 11/01/07 11:13 AM (16 years, 2 months ago)

not sure i would prolly keep it til it did something. never had it happen before.


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What if I am wrong, Ive got a condition, I get confused sometimes, what if I have imaged all of this, what if have finally turned into what they've always said I was gonna turn into.... a maniac...... a psycho killer.....Marv Sin City


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Invisiblemycocurious
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Registered: 02/09/07
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Re: When to pull the plug? [Re: Captain Cubensis]
    #7583648 - 11/01/07 11:15 AM (16 years, 2 months ago)

that pH is kind of high. I generally only amend the pH up to the 7.2 range and that's it.

That being said, three weeks is the longest I'd let a casing go before it became part of the landscape outside.


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figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.


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InvisibleCaptain Cubensis
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Registered: 09/18/07
Posts: 648
Re: When to pull the plug? [Re: mycocurious]
    #7583675 - 11/01/07 11:22 AM (16 years, 2 months ago)

Quote:

mycocurious said:
that pH is kind of high. I generally only amend the pH up to the 7.2 range and that's it.

That being said, three weeks is the longest I'd let a casing go before it became part of the landscape outside.




Well, yes, 7.9 is a little high, but usually the pH will drop in the first week, and having just checked it now, it reads right around 7.2

Also, there are no signs of contams, which really would have run rampant by now.

Perhaps the grains were not as thickly covered as they should have been. Yet, the jars were 100% colonized.


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Invisiblemycocurious
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Registered: 02/09/07
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Re: When to pull the plug? [Re: Captain Cubensis]
    #7583711 - 11/01/07 11:36 AM (16 years, 2 months ago)

Incidentally, when casing a grain that was not spawned to a bulk substrate I always allow my grains about a week of rest period after transferring them to the new trays to allow reconsolidate back into a single mass. After that I would case them and allow it to rest for another 3-5 days until I started to see mycelium poking through the casing layer and then off comes the cover and into the fruiting chamber they go - after one initial misting of the casing layer to replenish any moisture that was lost during the last few days.

After that, you can induce pinning...


--------------------
:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

- How Myco-Curious Prepares Coir & Compost Substrates
- How Myco-Curious Builds A Bulk Humidifier
- How Myco-Curious Builds An Automated Greenhouse
------------------------------------
figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.


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InvisibleCaptain Cubensis
Bleeding HeartLiberal


Registered: 09/18/07
Posts: 648
Re: When to pull the plug? [Re: mycocurious]
    #7583775 - 11/01/07 11:54 AM (16 years, 2 months ago)

Quote:

mycocurious said:
Incidentally, when casing a grain that was not spawned to a bulk substrate I always allow my grains about a week of rest period after transferring them to the new trays to allow reconsolidate back into a single mass. After that I would case them and allow it to rest for another 3-5 days until I started to see mycelium poking through the casing layer and then off comes the cover and into the fruiting chamber they go - after one initial misting of the casing layer to replenish any moisture that was lost during the last few days.

After that, you can induce pinning...




Hey man, thanks for the great advice, I will surely follow it in the future.

So, if there are no contams evident, then why would this take so long?

The casing is light and fluffy, without too much water, about 60% moisture content.

Has anyone had a similar issue with KSSS on cased rye berries?


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