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Offlinepsilopod
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The Beginning- substrain selection
    #7581218 - 10/31/07 03:12 PM (16 years, 3 months ago)

I wanted to post this into the grow logs but I don't have permission to post in that section of the forums, so here I begin.  I've had a few experiences over the years where I tried various little experiments w/ mushrooms, 2 of 3 being successful on a smaller scale.  I have just recently started up on a new grow and I am glad to say that I have taken it to another level in my own opinion.

This is currently a  joint project going on w/ a fellow enthusiast and general associate.  At present we have 3 pint jars fully colonized- South American, Brazilian, and Thai. Lipa Yai. Along w/ this is a spawn bag that's probably a little over a 1/2 gallon of WBS inoculated with United States Virgin Island, partially colonized quarts of Thai Mystery and Australian, and partially colonized pints of Argentinian as well as Ban Hua Thanon.

While these grains are spawning I also decided to try my hand at agar for the first time and do a little substrain selection.  I used the grocery store agar tek in the tiny half pint jars. On the agar I started Cambodian, Thai Mystery, South American, Brazilian, Hawaiin, Texas, and Puerto Rican.  So far the big winner has been South American which has been surprising.  I'm not sure but I think that it may be due to some slightly adverse conditions that took place in the form of below ideal temperatures during incubation for a short time period.  Regardless the South American has been the hands down winner so far from multiple innoculations.  I'm going to isolate some substrains on the agar and clone some of the nicer fruited mushrooms as well.  Although the South American has been the winner so far I have high hopes for most of these strains given ideal conditions which has been recently fixed(there was previously very little to no incubation) but now we have a full chest freezer at our disposal.  The thing is a good 6' long and 3' deep; it's a welcomed addition to the family:grin:.

Tonight we're inoculating 12 pint jars using mycelium water made from the colonized pint jars (south american, brazilian, thai. lipa yai).  I think I'm also gonna whip up another batch of agar but I have to check out another recipe to make so the cultures stay decent, so I guess it's off to the store tonight.  I might pick up some more lids and stuff while I'm there(got some plastic ones last time and don't like them that much). Almost forgot we just got some spawn bags so we just made up a bunch of them, 12 actually.  It's kind of a test run.  We're seeing which will be better bulk BRF or bulk Grain (WBS/Rye).  We're hoping that the bulk BRF bags do there thing as fast as the grain bags because the BRF bags are alot easier to prep.  I personally like the Rye.  I figure that if I can eat it, they can too.:stoned:  We made up 10 BRF bags and 2 Rye bags all somewhere around a 1/2 gal maybe a little bit more.

The spawn bags that we made up are getting spawned to trays and getting fruited in a green house.  I'm not sure what kind of trays we're going to use yet, I have a bunch of 12 qt sweater box sterilite containers. I have a Vicks coolmist humidifier (from target) and we picked up one of those ultrasonic fogger discs that you drop into water.  The only problem is that our ultrasonic device didn't come w/ one of those floating things that regulate the height of the disc.  This isn't really an issue for now with the gh but it will be in the future when I make one of agars large easy humidifier setups in a 5 gal bucket using giant airstones, industrial airpump, fogger disc, timer, aquarium heater, and squirrel cage fan w/ a couple pieces of pvc.  One thing at a time though(yeah right, lol).

Anyways, that's where I'm at for right now.  I'll have some picture to upload to you guys in a little bit after I get that whole thing figured out.  Other than that I have  a whole bunch of stuff to do so I'm gonna get going.

By the way I wanted to put this in the grow log thread but I don't have the ability as of yet or atleast I don't think I do.  If possible could one of the administrators move the thread at some point it would be appreciated, but if not I know that you guys will be busy and there will always be more to log:stoned:


Peace

P'funk


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This and all posts are the work of an imaginary character known as psilopod. Don't believe this rambling drivel, none of it's real, it's fake, it's an illusion, strictly for entertainment purposes. With that being said, enjoy.


Turning Dreams Into Reality



Edited by psilopod (11/09/07 08:47 AM)


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OfflineMarlboroMan
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Re: Getting on it- a Halloween adventure [Re: psilopod]
    #7581298 - 10/31/07 03:29 PM (16 years, 3 months ago)

Sounds good! Can't wait to see the pics! :rockon:


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I'll make it to the moon if I have to crawl..


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Offlinepsilopod
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Re: Getting on it- a Halloween adventure [Re: MarlboroMan]
    #7581718 - 10/31/07 05:36 PM (16 years, 3 months ago)

Thanks man.  I just noticed a little bit of contamination in my U.S.V.I. spawn bag.  It's nothing too major, but I'm not sure what I want to do with it.  I know that I could probably work around it and probably be successful but part of me doesn't even want to mess around w/ it.  I'll have a bunch more bags ready to go in a little bit so I'm thinking it might be best to not have any contams around.  Do you guys think that it is worth it to mess around w/? it's not trich it's a grey contaminant. I'm still a newb so I don't know all of the contams right off the rip and hopefully I won't become all that good at identifying them:stoned:  I'll definitely post up some pics.  We already took some pictures and we'll take some more tonight.


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This and all posts are the work of an imaginary character known as psilopod. Don't believe this rambling drivel, none of it's real, it's fake, it's an illusion, strictly for entertainment purposes. With that being said, enjoy.


Turning Dreams Into Reality



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Offlinepsilopod
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Re: Getting on it- a Halloween adventure [Re: psilopod]
    #7589299 - 11/02/07 08:00 PM (16 years, 2 months ago)

I've got an update for today, nothing too crazy but progress was made.  First off a couple of strains have been dropped from the line up due to a contamination and some slower performers.  Because of this I will not be running Argentinian, Australian, or U.S. Virgin Island.  On the other hand though I made up a bunch more grain and agar today, so hopefully things will be rockin' pretty soon.  'Cause I'm droppin' az couple strains I felt it only right to replace them :stoned: so I'll be starting up some B+, Yosterizi, and Amazonian. 

The agar is going well.  You can tell just by watching it that Puerto Rican is a beast; I have high hopes for PR and Hawaiian.  They are all performing well but I kind of have my hopes set on those two.  Only time and conditions will tell though.

The "new" incubator is holding temperature perfectly and everything seems to be on track except for my sleeping habit:crazy2: . Anyways, I'm kinda tired and just figured that I'd post a few pictures now that I have a little free time.  I should be able to get some good pictures going tomorrow as it's time for inoculation.  I have high hopes for the morning.

Sorry, nevermind about the pics for now, I just tried to load them and realized that I couldn't load .jpg . I'll post some pics up just not tonight, I'm beat.


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This and all posts are the work of an imaginary character known as psilopod. Don't believe this rambling drivel, none of it's real, it's fake, it's an illusion, strictly for entertainment purposes. With that being said, enjoy.


Turning Dreams Into Reality



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Offlineveda_sticks
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Re: Getting on it- a Halloween adventure [Re: psilopod]
    #7589331 - 11/02/07 08:11 PM (16 years, 2 months ago)

Nice, look forward to seeing pics of lipa yia, i have some going at the moment, there on cakes, its not going as good as the last cakes to fruit, but atleast i got some going



Its not much, seems to be a little less than the last cakes. But that 1 shroom is not showing any signs of cap opening, im homing that i can keep it growing a bit longer so its extra large and take a clone.

I injected 10cc,s of water into each cake 2day as they seemed to be getting a bit dehydrated. Heres hoping i have a monster shroom to eat!


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PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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Offlinepsilopod
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Re: Getting on it- a Halloween adventure [Re: veda_sticks]
    #7590125 - 11/03/07 02:42 AM (16 years, 2 months ago)

My Lipa Yai jar is super colonized at this point. It was not the fastest of the strains but it certainly did the most thorough job of consuming the grain. I have high hopes for Puerto Rican, Hawaiian,and Lipa Yai. All of the strains that I'm working with are supposed to be really good so as long as I get through these transfers I'm bound to have a winner of some sort.

Today I'll be knocking up the birdseed and various other spawns that we cooked up during the week. I'm still pretty new and every time I processed the grain I noticed something else that I could do that would improve my results. Because of this we ended up processing 51 1/2 pounds of spawn this week. 5150, the code for insanity; not that 50 lbs is that crazy but it's a decent amount of stuff. I almost went out and bought some more WBS too, I would have ended up w/ 71 1/2 lbs of stuff, lol. As of right now we have about 30 spawn bags give or take; the last 5 of them have to get pressure cooked this morning and then we are good to go.

We have 3 different types of spawn going as of right now: larg BRF cake, rye, and wbs. The large BRF cake was kind of an experiment to see how well it does in comparison to the grains. We were hoping to be able to use the brf because it is easier to prepare in a bulk quantity (from a standpoint of volume not weight)as I am going to use it for inoculation and not fruiting.

That's the next thing that will have to get worked on is the fruiting substrate. We basically took the poo w/ extra's recipe and just bulked it out using variations here and there. My thought process was that so long as the medium is pH and nutritionally balanced w/ the correct moisture content and properties the more complex the substrate the better for the mycelium.

So yah, today I will be taking my first transfers from the agar cultures. I made up like 16 more agar blanks yesterday and I already had a couple sitting around so I'm pretty well stocked now. Aside from the transfers I'm also going to start up another couple of strains today also as mentioned.

I have a bunch of pics that I cropped up that I want to load up on here but they're in .jpg format like I said so I have to go on the internet and see what I have to do to convert these bad boys. I'm hoping that it's something simple, I'm not a computer wiz kid by anymeans.


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This and all posts are the work of an imaginary character known as psilopod. Don't believe this rambling drivel, none of it's real, it's fake, it's an illusion, strictly for entertainment purposes. With that being said, enjoy.


Turning Dreams Into Reality



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Offlinepsilopod
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Re: Getting on it- a Halloween adventure [Re: psilopod]
    #7595634 - 11/04/07 06:06 PM (16 years, 2 months ago)

Well we just made up a whole bunch of mycelium water using colonized pint jars of wbs.  We made Lipa Yai, South American, Brazilian, and Ban Hua Thanon mycelium water.  We are using this water to knock up spawn bags of various spawn media(rye, wbs, brf).  We just got done knockin' up the first couple of bags and need to go to the store to stock up on some stuff. I have to figure out what I need to do to get my pictures up on here; they're in jpg format and won't work.

Already went to the store once today, picked up a bunch of wbs.  I saw a few new teks that made my whole approach to grain preparation alot easier especially when using the grain for spawning like we are, so I did up a little extra, lol  :bongload:


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This and all posts are the work of an imaginary character known as psilopod. Don't believe this rambling drivel, none of it's real, it's fake, it's an illusion, strictly for entertainment purposes. With that being said, enjoy.


Turning Dreams Into Reality



Edited by psilopod (11/04/07 06:09 PM)


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Offlinepsilopod
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Re: Getting on it- a Halloween adventure [Re: psilopod]
    #7596177 - 11/04/07 08:15 PM (16 years, 2 months ago)

Just knocked up a "couple" spawn bags.  I'm really digging it now that I have someone else to work with.  I've spent alot of time over the last few years going incognito by myself; it's nice to have someone else around. It makes things go alot faster as well, way more than twice as fast. We still have even more grain to knock up tomorrow that I made up quick today on a whim to test out a new tek(new to me), so there's a little excess if there ever was such a thing . :bongload: Anyways I still haven't figured out how to load up these pictures which is really annoying me.  I'm going to do a search on the subject but I don't understand it.  I try and attach files to my post but it says that I can't attach the jpg files.  I don't know if there's just something else that I have to do or if I'm going about it wrong but either way if anyone has any advice it would be greatly appreciated.


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This and all posts are the work of an imaginary character known as psilopod. Don't believe this rambling drivel, none of it's real, it's fake, it's an illusion, strictly for entertainment purposes. With that being said, enjoy.


Turning Dreams Into Reality



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Offlineurayasan
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Re: Getting on it- a Halloween adventure [Re: psilopod]
    #7596585 - 11/04/07 09:37 PM (16 years, 2 months ago)

To do the pictures you need to upload them to your account's images section, look for "Pics" somewhere up in the top left. After you upload some there, you will be given linking codes which you can paste into a post here to display them.


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Offlinepsilopod
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Re: Getting on it- a Halloween adventure [Re: urayasan]
    #7597064 - 11/05/07 12:08 AM (16 years, 2 months ago)

Thanks so much man, you have no idea how long I've been trying to get these pictures up on here. I wasn't spending my every waking moment on this problem but you definitely put a smile on my face when I checked the thread. Anyways, thanks again and here's some random pictures that I've got for you guys. I should have some more shots coming up for you guys tomorrow now that I know how to do this. Tomorrow I do my first agar transfer, process some more grain, and whatever else my buddy and I can get done.























Sorry there's not too much of a description going on right now but it's kind of late and the pictures are pretty self explanatory for the most part. If anyone wishes I can go more in depth into different subjects but for right now I'm just going to post these pics for people to get a look at.


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This and all posts are the work of an imaginary character known as psilopod. Don't believe this rambling drivel, none of it's real, it's fake, it's an illusion, strictly for entertainment purposes. With that being said, enjoy.


Turning Dreams Into Reality



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Offlineurayasan
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Re: Getting on it- a Halloween adventure [Re: psilopod]
    #7597125 - 11/05/07 12:27 AM (16 years, 2 months ago)

Very nice images, I'm so glad I could help!

The only question that really pops up for me is regarding your glovebox there, that locking lid looks identical to the style I'm accustomed to, but I have always found the lid to be a poor seal. The plastic just has too much play in it and it has been a bit of a bother in the past, I'm curious how you dealt with that, if it is even a problem for you. I have switched to putting the hand holes in the bottom of the tubs in order to minimize flexing on the lid during use, I've also used tape in the past on the lids that were particularly faulty in that regard...


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Offlinepsilopod
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Re: Getting on it- a Halloween adventure [Re: urayasan]
    #7597177 - 11/05/07 12:50 AM (16 years, 2 months ago)

I think that it really depends on what type of stuff you are doing.  Some things can be performed in open air and other things are a little bit more touchy.  Although I could get away w/ doing many of my procedures open air I choose to do them in a glove box.  Before I do all of this I make sure to clean the room pretty well.  So w/ the clean room the glove box is just kind of gravy and I don't really worry about the seals.

If you really wanted to fix that problem though I would suggest looking into weather stripping or things of that nature that weather proof homes.  You can find this at most hardware stores and I'm pretty sure walmart even has the stuff.  I personally don't think it's that big of a deal though.

As far as designs go, this g-box was not ideal and it was kind of a last minute fudged item to make due.  I have a larger glove box but the front of it got a hole broken into it so it's currently inoperable.  My current g-box has the holes through the lid and my old one had them through the side with a large hole cut into the lid and a piece of plexi-glass fitted.  The plexi-glass has since been used for another project and the container sits there.  Maybe I'll fix it someday but for now it's going to sit there.  But what I wanted to say is that I personally liked my older glove box better because it was 45  or 50 gallons so it had a lot of room, this new one is too small.  I couldn't find another clear container of that size in any of the stores near me.

Thanks for the compliments and thanks again for cleariing that issue up for me, you have no idea how happy that made me  :bongload:

Anyways, I'm gonna get going, just figured I'd say thanks again.  I'll have some more pics up tomorrow, oh no, today (it's now 2:47am).  Check back in, I have high hopes for the agar transfers tomorrow.  We'll also hopefully get a grain draining frame set up tomorrow as well.  I think that having a large heavy duty surface area to drain/strain stuff is invaluable to me at this point, I think that it will make a big difference.  It's all about the moisture content.


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This and all posts are the work of an imaginary character known as psilopod. Don't believe this rambling drivel, none of it's real, it's fake, it's an illusion, strictly for entertainment purposes. With that being said, enjoy.


Turning Dreams Into Reality



Edited by psilopod (11/05/07 12:53 AM)


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Offlineurayasan
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Re: Getting on it- a Halloween adventure [Re: psilopod]
    #7597181 - 11/05/07 12:54 AM (16 years, 2 months ago)

I'll be watching! Have a good night  :yourock:


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Offlinepsilopod
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Re: Getting on it- a Halloween adventure [Re: urayasan]
    #7597587 - 11/05/07 07:41 AM (16 years, 2 months ago)

Just a minor update, the heater that you see inside of that freezer will no longer be used as it's thermostat isn't that great and it's honestly a little too big for such a small, well insulated place. So, we're gonna throw a fish tank heater(mayber 2) inside of a 5 gal bucket and put that in the freezer. The only thing that kinda sucks is that it's a chest freezer so it lets heat out when you open it. Oh well, we'll see how it goes. For right now the stuff is just put in boxes in a room that has the heat turned up. We'd like to be a little more cost effective but you do what yah gotta do.

Here's the new heating setup for the freezer. It is yet to be tested but we're hoping that it works. Worst case scenario we might need to get a bigger fish tank heater or a bigger container for the water.







This is actually made w/ a 7 gallon bucket, not a 5 gallon bucket. Other than that it's just an aquarium heater and a small pump to circulate the water.


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This and all posts are the work of an imaginary character known as psilopod. Don't believe this rambling drivel, none of it's real, it's fake, it's an illusion, strictly for entertainment purposes. With that being said, enjoy.


Turning Dreams Into Reality



Edited by psilopod (11/05/07 01:39 PM)


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Offlinepsilopod
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Re: Getting on it- a Halloween adventure [Re: psilopod]
    #7597752 - 11/05/07 09:12 AM (16 years, 2 months ago)

I was wondering people's opinions on grain prep techniques. We're using our grain to spawn to bulk substrate. Because of this I've decided to make the grain as much on the dry side as I can and then I knock it up w/ a bunch of mycelium water, usually around 40-80cc per spawn bag which contains probably a little over a half gallon and an estimated 2 lbs dry weight of grain.

I was also wondering if anyone has had any experience with the inoculation guns that they sell at mycosupply. I remember reading a few limited posts on them but I was wondering if anyone out there had experience w/ them.


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This and all posts are the work of an imaginary character known as psilopod. Don't believe this rambling drivel, none of it's real, it's fake, it's an illusion, strictly for entertainment purposes. With that being said, enjoy.


Turning Dreams Into Reality



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Offlinepsilopod
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Re: Getting on it- a Halloween adventure [Re: psilopod]
    #7598602 - 11/05/07 01:54 PM (16 years, 2 months ago)

We're slowly working the kinks out of this entire process. This means building a large screen/strainer(4'x8'), ordering inoculation gun from vet supply place, last but not least we're in the process of figuring out a good way of bulk pasteurization. It would be cool to use the freezer to pasteurize the poo in one of the many ways agar has described unfortunately we only have one freezer. We still might be able to work around this but it's not ideal. For now we're thinking about using 5 gallon buckets to pasteurize the poo as the stores around here aren't carrying foam coolers right now ( a little out of season. We would insulate the containers some how, I'm not quite sure yet. Any suggestions are welcome.

I'd also appreciate some advice on pasteurization. I've read many of the teks and know the specifics of what needs to happen(temps and times required) but I don't know the general of it. I know that I should hold the poo (core temp) in the 160-165 range for an hour, but I want to know what temp water I can dump in to a container to accomplish this. Again I will search this more, but any advice would be appreciated.


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This and all posts are the work of an imaginary character known as psilopod. Don't believe this rambling drivel, none of it's real, it's fake, it's an illusion, strictly for entertainment purposes. With that being said, enjoy.


Turning Dreams Into Reality



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Re: Getting on it- a Halloween adventure [Re: psilopod]
    #7598672 - 11/05/07 02:20 PM (16 years, 2 months ago)

Quote:

ordering inoculation gun from vet supply place,





please expound?

you must elucidate!


Edited by jeetered (11/05/07 02:20 PM)


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Offlinepsilopod
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Re: Getting on it- a Halloween adventure [Re: jeetered]
    #7598781 - 11/05/07 02:53 PM (16 years, 2 months ago)

I'm on a little bit of a budget so we didn't order the gun from a sponsor's site. I'm usually all about it but this time I found there to be a significant difference in pricing so I steered in the other direction. I won't give any specifics but do a search on the internet for pistol grip syringes, inoculation guns, socorex, and whatever other related terms you find in your searches. The guns are anywhere from $20-$200. We decided to order one that's $60 and do 10ml inoculations continuously fed from an lc w/ a large needle and silicone tubing. These instruments are generally used for mass vaccination of animals, it's a veterinary tool.



hope that helps, if not just pm me


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This and all posts are the work of an imaginary character known as psilopod. Don't believe this rambling drivel, none of it's real, it's fake, it's an illusion, strictly for entertainment purposes. With that being said, enjoy.


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OfflineBlargIAmDead
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Re: Getting on it- a Halloween adventure [Re: psilopod]
    #7599325 - 11/05/07 05:13 PM (16 years, 2 months ago)

:eek: That is a sexy sexy device you have there psilopod. Almost makes me want to go out and vaccinate people :smile:. It seems like a pretty good way of doing things. Save time on making syringes, no worries around needle transfer between the LC jar and bag. Just remember to sterilize between each bag :smile:.


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Offlinepsilopod
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Re: Getting on it- a Halloween adventure [Re: BlargIAmDead]
    #7599597 - 11/05/07 06:21 PM (16 years, 2 months ago)

I'm planning on using alcohol soaked makeup sponges to sheath the needle that way it has minimal air exposure and is constantly being disinfected w/ alcohol.  You just have to be careful not to get alcohol in your spawn or anything else of importance.  I should mention that I don't have it as of yet, it will be here within a couple of days.

Aside from that I'd like to update this thread w/ some new pics.  We built a screen/strainer out of some scrap wood and screen slapped together.  I won't throw out any numbers but there's a decent amount of grain spread across this screen.  The screen itself is 4'x7' for reference :bongload:

I'll also mention that there's a dehumidifier going right by this and that there sill soon be a fan located next to it for air circulation.













Edited by psilopod (11/05/07 06:24 PM)


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