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Offlineresptodd
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Registered: 10/16/07
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poll grain-vs-lc
    #7565138 - 10/26/07 11:07 PM (16 years, 3 months ago)

I be a new bee. So check it out. I've done all my PF tek jars, very happy with my sterile procedures, look forward to some success.---Now I have 2-3ml left in a syringe. Should I innoc. grain jar or lc? This is real life, not paper. Rye berries best grain?--- Karo syrup best lc? What are todays best chances---(given sterile tek will not be an issue, I always cross my fingers)


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InvisibleThis guy
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Re: poll grain-vs-lc [Re: resptodd]
    #7565215 - 10/26/07 11:40 PM (16 years, 3 months ago)

if you did extensive searching you would know the answer.

but since i've spent MONTHS going through archives.. i'll let you in on a secret.

dont inject spores into LC. thats not a great way to do things, as most of the time.. you're more than likely to get contams.

knock up one jar with about half the jar full of Rye berries (which everyone says are the best).. let it fully colonize..

then.. pay close attention now..

once fully colonized.. prepare yourself a jar of distilled or spring water.. all you need is half a jar.. pressure cook it for an hour and make sure its sterile.

once thats done and cooled off.. get out your glove box.. do your sterile technique.. and under sterile conditions pour that sterile water into the colonized rye jar.. dont fill the jar too much.. and be quick...

then shake the berries up with the water in it.. brea loose the mycellium then pour the water back into the sterile water jar. now you have some serious LC which you can use to inoculate LC's or just put right into syringes to innoc more jars.

i hope thats not too confusing...

this way, you know for sure that there are no contams... just make sure you shake the jars AFTER you put the sterile water in.

also, after you drain the rye berry jar, you can recap and incubate and it will recolonize.. you can do it again and again. its a pretty flawless technique to get unlimited amounts of mycellium water.

then store in fridge and use whenver you need it.


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InvisibleThis guy
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Re: poll grain-vs-lc [Re: This guy]
    #7565220 - 10/26/07 11:41 PM (16 years, 3 months ago)

also if you have trouble finding rye berries.. such as myself.. WBS is the way to go. 40lb bags for 8 bucks you cant beat it. just make sure to get most of the black sunflower seeds out.. you dont need all them out.

just follow the teks and you shouldnt see failure ever. and no shortcuts. big no no IME


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Offlinetheratatat
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Re: poll grain-vs-lc [Re: resptodd]
    #7565221 - 10/26/07 11:42 PM (16 years, 3 months ago)

Knock up a grain jar with the spores. When its fully colonized inject the grain jar with a good amount of sterile water. Shake it up really well and suck it back out. Now use that water to knock up many jars or knock up an LC.

I forget who's tek that is, but I give them props none the less :smile:

Cheers


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Offlinetheratatat
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Re: poll grain-vs-lc [Re: theratatat]
    #7565237 - 10/26/07 11:45 PM (16 years, 3 months ago)

Damn beat me to it with a better description. :smile:


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InvisibleThis guy
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Re: poll grain-vs-lc [Re: theratatat]
    #7565265 - 10/26/07 11:52 PM (16 years, 3 months ago)

hahahah score!

and i think its agars tek.. that or RR's. either way.. its the best/only way to go


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Offlineresptodd
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Re: poll grain-vs-lc [Re: This guy]
    #7566191 - 10/27/07 09:39 AM (16 years, 3 months ago)

Hey, ya' talked me into it, thnx. Pretty much straight forward. That's what I was looking for, real life experience. Thanx again.


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Invisiblemycocurious
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Re: poll grain-vs-lc [Re: resptodd]
    #7566640 - 10/27/07 12:24 PM (16 years, 3 months ago)

You wouldn't recap and reuse the grain master over and over again, you'd use it _as_ a grain master in a grain to grain transfer.

1. Select a healthy colonized rye grain jar to be your grain master.
2. Prepare and sterilize 20 new rye grain jars in the normal fashion.
3. Prepare and sterilize a 30cc or so of water.

...During the very++ sterilize grain to grain transfer process...

4. Break up the grains in the grain master
5. Pour in the sterilized water
6. Swirl the grains and water around for a second or two
7. Draw up the new "liquid culture" into sterile syringes, cap and set aside.
8. Finish the grain-to-grain transfer in the normal fashion.
9. Use the LC syringes to inoculate the other 10 jars you have.

---
30cc of water will net you about two 10cc syringes as some water will not drain back out of the grains... Obviously being consistent in your jar's moisture content and not having them be too wet to begin with makes everything that much easier but yeah, it's a cheap and effective trick to have one jar inoculate 20 new jars.


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:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

- How Myco-Curious Prepares Coir & Compost Substrates
- How Myco-Curious Builds A Bulk Humidifier
- How Myco-Curious Builds An Automated Greenhouse
------------------------------------
figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.


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Re: poll grain-vs-lc [Re: mycocurious]
    #7566714 - 10/27/07 12:53 PM (16 years, 3 months ago)

ah good point. i never used the master grain jar as a grain to grain. i usually just do step one.. then let it recolonize.. then do it one more time and by then i just toss it. maybe i'll start doing that.


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Offlinesoulsizzle
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Re: poll grain-vs-lc [Re: mycocurious]
    #7566718 - 10/27/07 12:55 PM (16 years, 3 months ago)

Just my 2 cents. An hour in the pressure cooker is overkill when sterilizing water. Liquids sterilize much more easily than grains. A half hour should be more than enough. Even a half hour is a little more than necessary, but good for piece of mind.


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InvisibleThis guy
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Re: poll grain-vs-lc [Re: soulsizzle]
    #7566749 - 10/27/07 01:07 PM (16 years, 3 months ago)

yea very true. hour is overkill.. thats just how i do it.. heck, even 15 min is enough.


Edited by This guy (10/27/07 01:09 PM)


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