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Offlineinet
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Registered: 10/26/07
Posts: 7
Last seen: 16 years, 29 days
probable contamination
    #7565174 - 10/26/07 11:22 PM (16 years, 3 months ago)

at 85% colonization purple contaminations have shown up.
looks to be neurospora.

i moved the contaminated jars to a sealed tub away from my clean stock
so i guess we will see in a couple of days.

my main incubator where my clean cultures are still being kept was cleaned with a 0.65% active solution of bleach. then the clean cultures were returned.

7 out of 8 culture jars showed contamination, where as none of the 8 other jars inoculated with a different syringe show contamination.

which leads me to believe it might have been a bad spore syringe.

any thoughts / opinions on this ?


** Update **

i checked the last clean jar of the batch and found a purple contamination, so that makes it 8 out of 8.

looks like i got lucky and it didn't spread to my other 8 jars.



Edited by inet (10/27/07 11:54 PM)


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Invisiblelegallyhomeless
mooch
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Registered: 07/01/05
Posts: 4,051
Loc: EZRA for the Refuge
Re: probable contamination [Re: inet]
    #7582308 - 10/31/07 08:36 PM (16 years, 3 months ago)

that doesnt look like myc too me.


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OfflineCaribou_Lou
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Registered: 10/17/07
Posts: 2,510
Loc: Never Land
Last seen: 12 years, 10 months
Re: probable contamination [Re: legallyhomeless]
    #7584808 - 11/01/07 05:14 PM (16 years, 2 months ago)

I'm sure it's just his camera, what else would it be lol?


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OfflineRogerRabbitM
Bans for Pleasure
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: probable contamination [Re: Caribou_Lou]
    #7585323 - 11/01/07 07:36 PM (16 years, 2 months ago)

Contamination rarely spreads from jar to jar because of the filters. I'd suggest getting rid of the incubator and colonizing jars out in the open at normal room temperature. Thermophilic molds and bacteria are stimulated at high temperatures, while mushroom mycelium grows just fine at 75F.
RR


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Offlineinet
Stranger
Registered: 10/26/07
Posts: 7
Last seen: 16 years, 29 days
Re: probable contamination [Re: RogerRabbit]
    #7586125 - 11/01/07 11:37 PM (16 years, 2 months ago)

RR, thanks for the advice.

I think I'm going to set the temp control on my incubator to 75 degrees, as my room sometimes drops below 60.

The only part that strikes me as odd is all 8 jars were contaminated.


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