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Offlineshabooboo
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LC or straight to jars?
    #7565163 - 10/26/07 11:19 PM (16 years, 3 months ago)

OK i just received my syringe of Cambodian strain cubes. And 10 Pre-sterile Rye quart jars. So i was wondering would it be better to start a LC (i was thinking honey if so) or just put 1CC of the multispore in each quart jar.
And another thing my syringe that i have (from a sponsor here so i trust) Is totally clear but i think thats OK? from what I've read. But i didn't know if only 1cc per jar of a syringe this clear would be OK too?
Any thoughts?


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InvisibleThis guy
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Re: LC or straight to jars? [Re: shabooboo]
    #7565276 - 10/26/07 11:54 PM (16 years, 3 months ago)

ehh.. many will argue this subject. but injecting spores into honey water or any LC will half of the time lead to contams. check out this post. since i dont want to type it again...

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/7565138/an/0/page/0/gonew/1#UNREAD


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InvisibleThis guy
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Re: LC or straight to jars? [Re: This guy]
    #7565284 - 10/26/07 11:56 PM (16 years, 3 months ago)

also.. my B+ syringe looked clear.. and they TOOK OFF after incubating for a couple days. you dont have to see spores for them to be there...


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OfflineCaribou_Lou
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Re: LC or straight to jars? [Re: This guy]
    #7565425 - 10/27/07 12:46 AM (16 years, 3 months ago)

No LC is great and if you do it right it will not get contaminated.. there's no reason it would.


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InvisibleThis guy
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Re: LC or straight to jars? [Re: Caribou_Lou]
    #7565440 - 10/27/07 12:51 AM (16 years, 3 months ago)

yea LC's always look great.. untill you knock up a jar and its pure green. hah!

not trying to argue. but the way i mentioned... which has been gathered from either agar or RR.. its pretty much failsafe... like i said. half the time i did spores in an LC.. i got contams.. even though there werent any visible contams in the LC. its crazy how it works.. i know.

to each their own. me? i prefer not to waste my spores on nutritious water.


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OfflineCaribou_Lou
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Re: LC or straight to jars? [Re: This guy]
    #7565452 - 10/27/07 12:56 AM (16 years, 3 months ago)

do you use a glovebox and PC?


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InvisibleThis guy
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Re: LC or straight to jars? [Re: Caribou_Lou]
    #7565457 - 10/27/07 12:58 AM (16 years, 3 months ago)

lol of course


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OfflinekingboomerDiscord
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Re: LC or straight to jars? [Re: This guy]
    #7565518 - 10/27/07 01:32 AM (16 years, 3 months ago)

Just use 1cc for your LC, use the rest for a multispore.  If the LC fails, you still have jars that will do fine.  Count it as practice? :shrug:


-Kingboomer


--------------------
Hole in the sky/
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through it I'll fly...

-Black Sabbath


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Offlineveda_sticks
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Re: LC or straight to jars? [Re: kingboomer]
    #7565776 - 10/27/07 05:04 AM (16 years, 3 months ago)

LC,s are harder to spot contams, but if done right, can make a spore syringe go a long way. If u get it right, then u can try making an LC from a clone, keep that as a master culture and make another working LC from the master, then come back to the master once u need more LC from that master.

Its odd how u had so much trouble with LC, my first LC came out fine even though i ended up having to expose it to air. I got 1 syringe half a syringe out of it and thought, its gonna contam, but ended up with a few good jars and no contams.

I think thats not great advice saying, no dont bother they contam.

U can only try. Nothing to loose, except 1 cc of spore solution. If it fails then u still have jars colinising that u could take a sample of myc from into a syringe and knock up more jars.

I read your post about filling a jar with distilled water into colinised rye shake and back into the original jar for liquid culture, this method i dont doubt works as i know it does.

Doesn't it make sense that by opening ur jar and doing those transferes is going to have more of a chance of contaming - than taking a syringe, flame sterilising and alcohol swabbing it, then injecting into an LC threw a silicon injector port?

With an LC Jar properly made properly, there is very little chance of contams making it into your media.


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PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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InvisibleThis guy
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Re: LC or straight to jars? [Re: veda_sticks]
    #7566091 - 10/27/07 08:49 AM (16 years, 3 months ago)

like stated in the link above... doing the method i mentioned is a failsafe way to ensure no contams...since you can visually check the colonized jar for such nasties beforehand.

i personally hate LC's the direct spore way.. unless you have a stir plate and get the content perfect.. its more than likely going to suck. im not saying it will never work..never said that.. i guess im just biased towards teks that have worked for me 100% of the time..

call me crazy.


by the way.. when you have a fully colonized jar.. your chances of having that get contaminated due to open air exposure are very slim..due to the fact that the myc already has the upper hand..

this is all information that can be found everywhere on this board.. i dont know why im always repeating myself.


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Invisibleorchidfanatic
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Re: LC or straight to jars? [Re: This guy]
    #7566095 - 10/27/07 08:52 AM (16 years, 3 months ago)

When I make an LC I check whether its contaminated or not by putting some on agar growing it out and check for contaminates. Then I can inoculate things with it with a low chance of contamination


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InvisibleThis guy
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Re: LC or straight to jars? [Re: orchidfanatic]
    #7566109 - 10/27/07 09:03 AM (16 years, 3 months ago)

Quote:

orchidfanatic said:
When I make an LC I check whether its contaminated or not by putting some on agar growing it out and check for contaminates. Then I can inoculate things with it with a low chance of contamination




:thumbup:


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Offlineveda_sticks
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Re: LC or straight to jars? [Re: This guy]
    #7566125 - 10/27/07 09:10 AM (16 years, 3 months ago)

one of the great things about agar.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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Offlineshabooboo
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Re: LC or straight to jars? [Re: veda_sticks]
    #7566756 - 10/27/07 01:10 PM (16 years, 3 months ago)

ok so i think I'm just going to knock the jars up with the multispore. Then i can make a LC when i have a jar colonized. This way i can just get it started too! (tomorrow). I'm assuming 1cc is enough for each jar right?

thanks for the input by the way


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InvisibleThis guy
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Re: LC or straight to jars? [Re: shabooboo]
    #7566779 - 10/27/07 01:18 PM (16 years, 3 months ago)

a way to get the most out of your syringe.. (if you want to use spores for your LC).

go ahead and knock up your jars with the spores.. then make a honey water solution or whatever you choose to use...

then suck up some honey water in the syringe.. there are always spores left behind in the syringe. more than u'd think.

there are so many ways to do things. hopefully you find one you like and can get good success with. 1cc is enough. may take longer than 2cc's but it'll eventually come 'round


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Offlinesoulsizzle
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Re: LC or straight to jars? [Re: This guy]
    #7566817 - 10/27/07 01:34 PM (16 years, 3 months ago)

You definitely want to inoculate your jars ASAP, regardless of whether or not you create a LC as well. Sterilized jars should be used immediately. Every day you wait is another window of opportunity for contams to get a foothold.


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