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OfflineJazzCatCF
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isolating
    #7563673 - 10/26/07 03:06 PM (16 years, 3 months ago)

Im about to start isolating for my 1st time.I was wondering if I could just use a piece of colonized grain on the agar.


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"There is a world beyond ours, a world that is far away, nearby, and invisible. And there it is where God lives, where the dead live, the spirits and the saints, a world where everything has already happened and everything is known. That world talks. It has a language of its own. I report what it says. The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me. When I return from the trip that I have taken with them, I tell what they have told me and what they have shown me."

The Mazatec Wise Woman, Maria Sabina (1894-1985)


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OfflineFraggin
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Re: isolating [Re: JazzCatCF]
    #7563680 - 10/26/07 03:08 PM (16 years, 3 months ago)

You can. To start out with, but expect to go through several petris of agar.
I'm not that great at isolating, but it is tedious work....
Get your sterile methods figured out and practice a few and you should get the hang of it.


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Invisiblemonstermitch
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Re: isolating [Re: JazzCatCF]
    #7563684 - 10/26/07 03:08 PM (16 years, 3 months ago)

that could work. just make the transfer

I hope you have dozens of petri dishes poured.
isolating from multispore is a very long process
demanding dozens of petri dishes.


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OfflineJazzCatCF
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Re: isolating [Re: monstermitch]
    #7563734 - 10/26/07 03:33 PM (16 years, 3 months ago)

Realy?Man,rr makes it look easy in his video,bnut I guess nothing comes without failure in this hobby.maybe thats why I love it so much:heart:


--------------------
"There is a world beyond ours, a world that is far away, nearby, and invisible. And there it is where God lives, where the dead live, the spirits and the saints, a world where everything has already happened and everything is known. That world talks. It has a language of its own. I report what it says. The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me. When I return from the trip that I have taken with them, I tell what they have told me and what they have shown me."

The Mazatec Wise Woman, Maria Sabina (1894-1985)


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Invisibleorchidfanatic
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Re: isolating [Re: JazzCatCF]
    #7563789 - 10/26/07 04:00 PM (16 years, 3 months ago)

I have have created 1 mono culture in about 15 agar plates .. some may take longer.. and yes you can start an agar plate with a kernel .. I started a culture slant for storage with a kernel .


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Invisiblemonstermitch
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Re: isolating [Re: orchidfanatic]
    #7563801 - 10/26/07 04:07 PM (16 years, 3 months ago)

it'll take a few (3 or more) transfers usually to get to monocultures.

but the trouble is that you'll need probably 10 or so monocultures
independent of one another. each and every one will then
be fruited and only the one with the correct traits will be kept.
and there's a good chance that none of them will have the
correct traits. so then the process will have to start over again.
on and on until a strain is found that grows and produces in your
environment to your set of standards.

so in the end, gobs of petri dishes are used.
it's all worth it though...


oh...
make sure to label everything very well, in a way you can't
just rub the label off either.
labeling is key to this process.


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OfflineJazzCatCF
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Re: isolating [Re: monstermitch]
    #7563997 - 10/26/07 05:09 PM (16 years, 3 months ago)

Ok,something im unclear of though is,say I get a monoculture that is an incredible fruiter,well if ive already used the agar wedge for the grain, what do I use for the slant.(probably a dumb ? but sometimes common sense takes a while to hit me):crazy2:


--------------------
"There is a world beyond ours, a world that is far away, nearby, and invisible. And there it is where God lives, where the dead live, the spirits and the saints, a world where everything has already happened and everything is known. That world talks. It has a language of its own. I report what it says. The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me. When I return from the trip that I have taken with them, I tell what they have told me and what they have shown me."

The Mazatec Wise Woman, Maria Sabina (1894-1985)


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Invisiblemonstermitch
Growing in Bags Doesn't Work


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Re: isolating [Re: JazzCatCF]
    #7564108 - 10/26/07 05:35 PM (16 years, 3 months ago)

another agar wedge from that original plate.

one plate can produce lots of wedges.
most of my transfer wedges are very small. like a grain of rice.

once you've found the strain you want, you go back to that
plate and work from it only. tossing all of the other strains out
that didn't do so well. make a master LC, slants, and whatever
you need to from that plate.

that's why labeling is so critical.
you need to match the fruiting trays with the plates the culture
came from.


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OfflineJazzCatCF
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Re: isolating [Re: monstermitch]
    #7566124 - 10/27/07 09:10 AM (16 years, 3 months ago)

Ok,so I would make transfers b4 I start a grain master,and then when one of the g2g shows great growth I would go back to the transfer plate and make a slant.Also,IM actualy starting today with tissue culture (EQ)Ill be working in a semi closed glove box with tryethylene glycol burning.Works perfect for g2g.No contams after 3 different transfers.


--------------------
"There is a world beyond ours, a world that is far away, nearby, and invisible. And there it is where God lives, where the dead live, the spirits and the saints, a world where everything has already happened and everything is known. That world talks. It has a language of its own. I report what it says. The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me. When I return from the trip that I have taken with them, I tell what they have told me and what they have shown me."

The Mazatec Wise Woman, Maria Sabina (1894-1985)


Edited by JazzCatCF (10/27/07 09:14 AM)


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Invisibleorchidfanatic
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Re: isolating [Re: JazzCatCF]
    #7566174 - 10/27/07 09:33 AM (16 years, 3 months ago)

also remember if a plate shows great rhizmorphic growth then that isolate WILL fruit. Thats according to RR.


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OfflineJazzCatCF
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Registered: 10/01/06
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Re: isolating [Re: orchidfanatic]
    #7566283 - 10/27/07 10:16 AM (16 years, 3 months ago)

Alright Orchid,Thanx,Thats what Ill be looking for.I plan on doing it just like in the video minus the flow hood.Wont be getting that till after taxes:grin:


--------------------
"There is a world beyond ours, a world that is far away, nearby, and invisible. And there it is where God lives, where the dead live, the spirits and the saints, a world where everything has already happened and everything is known. That world talks. It has a language of its own. I report what it says. The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me. When I return from the trip that I have taken with them, I tell what they have told me and what they have shown me."

The Mazatec Wise Woman, Maria Sabina (1894-1985)


Extras: Filter Print Post Top
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