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InvisibleSlimz
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Registered: 10/03/07
Posts: 3,588
Loc: Maryland
Making a syringe
    #7561967 - 10/26/07 09:03 AM (13 years, 6 months ago)

Ok so i did a little UTFSE and came up with some ideas, but here is the dilemma.

I have dogs, and very lived in house. Its not dirty, but im sure there is PLENTY around to contaminate my syringe.

I have a nice print sitting in an envelope on foil (thanks Jeet) and want to make it a syringe.

My thought was this.

PC the syringe, a shot glass, turkey baster
boil some distilled water
clear table completely.
cover an area in saran wrap or foil
clean it with a 10% bleach solution (or alcohol?)
wash hands arms
wear gloves
place shot glass on table
fill it with boiling water (using a turkey baster)
cover with alum foil and allow to cool
Lysol area
open print foil and scrape spores in to now cooled water with tip of syringe
suck up spores.


Suggestions...


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Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

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Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

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OfflineGroomies
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Re: Making a syringe [Re: Slimz]
    #7561984 - 10/26/07 09:18 AM (13 years, 6 months ago)

careful with the distilled water. i heard it doesn't boil so it might pop in your face and burn the shit out of you.


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:hotidea::hotidea::hotidea:


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Offlinecliffton hanger
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Registered: 09/17/07
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Re: Making a syringe [Re: Groomies]
    #7561993 - 10/26/07 09:21 AM (13 years, 6 months ago)

clean the smallest room you can fit in with gear(bathroom or closet)
that would be my suggestion.I also have dogs and a lived in house, never had any big problems with contams.(once in a great while)
good luck:cool:


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InvisibleSlimz
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Re: Making a syringe [Re: Groomies]
    #7562071 - 10/26/07 10:00 AM (13 years, 6 months ago)

Quote:

Groomies said:
careful with the distilled water. i heard it doesn't boil so it might pop in your face and burn the shit out of you.




Thanks for your concern. but just so you know...

{from Wikipedia}
A popular myth about distilled water is that it has the dangerous property of being more easily heated above its normal boiling point without actually boiling (as seen in Mythbusters) in a process known as superheating. When superheated water is disturbed or has impurities added to it, a nucleation center for bubbles form. These bubbles are then new nucleation centers, and a sudden, explosive boiling can occur, possibly causing serious injury to those nearby. However, distilled water and tap water do not differ in their ease of or danger in being superheated. The dissolved impurities in motionless tap water do not present enough disturbance to inhibit superheating.


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


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Invisiblemycocurious
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Re: Making a syringe [Re: Slimz]
    #7562349 - 10/26/07 11:47 AM (13 years, 6 months ago)

when transferring the spores off your print you would have a much lower damage rate if you were too...

1 - get an inoculation loop. if you can't, go to the cosmetics section of your local pharmacy and look for a "black-head remover" tool. Fundementally, they're they same damn thing.

2 - flame sterilize the inoculation loop until it's red hot for a second and then drop it into your sterilized water and use it to pull out a small bead of water.

3 - drop that bead of water on the print and gently swirl it around for a couple seconds with the inoculation loop and then drag that bead of spores back into your shotglass.

----
Using a sharp edge against the dry spores will cut, tear and otherwise damage a good deal of them rendering them useless for colonization...


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:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

- How Myco-Curious Prepares Coir & Compost Substrates
- How Myco-Curious Builds A Bulk Humidifier
- How Myco-Curious Builds An Automated Greenhouse
------------------------------------
figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.


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InvisibleSlimz
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Re: Making a syringe [Re: mycocurious]
    #7562360 - 10/26/07 11:52 AM (13 years, 6 months ago)

actualy i was thinking of just laying the print over the shotglass (print tward water) and giving it a flick/tap... the print is the perfect size ...


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


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OfflineRogerRabbitM
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Re: Making a syringe [Re: Slimz]
    #7562372 - 10/26/07 11:55 AM (13 years, 6 months ago)

Quote:

and giving it a flick/tap




That would drop god only knows what into your solution. Lots of contaminant spores are all over prints, because there's no such thing as a sterile print. Use an inoculating loop to get a small amount of spores off the top layer of the print.
RR


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InvisibleSlimz
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Re: Making a syringe [Re: RogerRabbit]
    #7562376 - 10/26/07 11:56 AM (13 years, 6 months ago)

ok.. ill do that...


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


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Invisibleblood4blood
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Registered: 04/26/07
Posts: 6,029
Loc: The Valley
Re: Making a syringe [Re: Slimz]
    #7563339 - 10/26/07 03:14 PM (13 years, 6 months ago)

build a glovebox. problem solved. best damn advice that was ever given to me. no contams since i built that thing.


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