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Offlineozzyozzyozzy
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Is my WBS spawn bag too dry?
    #7560987 - 10/25/07 09:58 PM (16 years, 5 months ago)

Here's the picture 4 days post-LC inoc:

http://img135.imageshack.us/img135/2345/s5000687pe8.jpg

There's a lot mycelium on the bottom, but it's clustered around where I sprayed the LC and where the liquid would have collected on the bottom. It doesn't seem to be spreading as fast as I might have expected, and it's gravitating towards the walls of the bag where the most condensation would occur. I'm wondering if it looks like the grains are too dry for optimum growth - should I inject another syringe-load [of the same LC] onto the back, plus some more sterilised water? Or would that just complicate things.

It's now inoc+6 days or so, and the growth isn't much better. It's growing, sure, but much less competently than I'd expect from a healthy spawn bag.

Edited by ozzyozzyozzy (10/25/07 09:59 PM)

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Invisiblemonstermitch
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Re: Is my WBS spawn bag too dry? [Re: ozzyozzyozzy]
    #7561303 - 10/25/07 11:16 PM (16 years, 5 months ago)

if anything it looks too wet.

I think I can see moisture on the surface of the grains
down at the bottom of the bag.

the water in spawn should be inside of the grains, not outside.
the outsides of the grains should be dry.

the bottom of the bag should not have any more moisture than
the side or the top of the bag, if so there's too much water in there.

so too dry? I would say no.
but that's just my thoughts... I'm sure some will not agree.


what you should have done is stuck the needle in above the
grain and spray down the sides of the bag and on the top of
the grain. that would provide you best colonization times.

so my advice is to just chill out.
don't touch the bag for another 4-6 days, when it'll probably
be ready for a massage.


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Offlineozzyozzyozzy
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Re: Is my WBS spawn bag too dry? [Re: monstermitch]
    #7561335 - 10/25/07 11:27 PM (16 years, 5 months ago)

the bottom/sides of the bag have moisture because i took a full load of LC [12mls] including water/honey and injected it in. it should balance out soon enough because the rest of my grain is kind of dry, i drained it pretty well and only soaked 24 hours

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OfflineOatman2000
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Re: Is my WBS spawn bag too dry? [Re: ozzyozzyozzy]
    #7561385 - 10/25/07 11:46 PM (16 years, 5 months ago)

good to me!


--------------------
Spawning to COIR
:thumbup:  My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
----------------------------

4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine

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Invisiblemonstermitch
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Re: Is my WBS spawn bag too dry? [Re: ozzyozzyozzy]
    #7561388 - 10/25/07 11:47 PM (16 years, 5 months ago)

did you TEST it before you loaded it in the bags??


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Offlineozzyozzyozzy
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Re: Is my WBS spawn bag too dry? [Re: monstermitch]
    #7561604 - 10/26/07 01:30 AM (16 years, 5 months ago)

Yes I did, but the LC would offset it a bit.

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Invisiblemonstermitch
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Re: Is my WBS spawn bag too dry? [Re: ozzyozzyozzy]
    #7562448 - 10/26/07 10:19 AM (16 years, 5 months ago)

it should be just fine then.


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Offlinesoulsizzle
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Re: Is my WBS spawn bag too dry? [Re: monstermitch]
    #7563274 - 10/26/07 12:56 PM (16 years, 5 months ago)

If you plan on spawning this to a bulk substrate, then it is generally good to keep your grains a little dry. Keeping them on the dry side will cause it to colonize quicker due to the fact that the mycelium needs to branch out and look for more moisture.


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Offlineozzyozzyozzy
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Re: Is my WBS spawn bag too dry? [Re: soulsizzle]
    #7571257 - 10/28/07 10:11 PM (16 years, 5 months ago)

Hmm alright. It's been about 8 days so far and I've been massaging/mixing the colonized areas every two days or so, which probably isn't a good idea so I'm going to stop til a lot more has been colonized. But out of interest, what's the average/commonly found colonization rates for a 1.5kg WBS spawn bag? Say it's cubensis.

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Offlinesoulsizzle
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Re: Is my WBS spawn bag too dry? [Re: ozzyozzyozzy]
    #7580231 - 10/31/07 11:11 AM (16 years, 4 months ago)

Quote:

ozzyozzyozzy said:
Hmm alright. It's been about 8 days so far and I've been massaging/mixing the colonized areas every two days or so, which probably isn't a good idea so I'm going to stop til a lot more has been colonized. But out of interest, what's the average/commonly found colonization rates for a 1.5kg WBS spawn bag? Say it's cubensis.




Every time you you break up the mycelium, you are damaging it. It then has to use additional energy to recover. Therefore, by "massaging/mixing" every two days, you are doing more harm than good. You should only do this once at about 15% colonization. Just let it colonize on its own now.


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OfflineAndrew47
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Re: Is my WBS spawn bag too dry? [Re: soulsizzle]
    #7580394 - 10/31/07 11:46 AM (16 years, 4 months ago)

Yeah, leave that bag alone!!! Stop playing with it and it'll do wonders for ya


--------------------
It's easy! Send your clean prints to www.fsrcanada.com

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Offlineozzyozzyozzy
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Re: Is my WBS spawn bag too dry? [Re: Andrew47]
    #7582014 - 10/31/07 06:59 PM (16 years, 4 months ago)

Alright, alright, I'm leaving it. It's just so tempting to become involved in the colonization instead of just sitting back and watching it. I'll just leave it from here on out though. I was thinking of spawning it to coco coir and casing with the 50/50+ casing tek [but no bottom layer]. Is there anything easier / better? I can't use hpoo.

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InvisibleThis guy
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Re: Is my WBS spawn bag too dry? [Re: ozzyozzyozzy]
    #7582232 - 10/31/07 08:06 PM (16 years, 4 months ago)

Quote:

ozzyozzyozzy said:
Alright, alright, I'm leaving it. It's just so tempting to become involved in the colonization instead of just sitting back and watching it. I'll just leave it from here on out though.




haha tell me about it..

Im a cigarette smoker so you'd have to be one to understand this..

but mycellium is on my mind more than cigarettes are. its crazy

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Offlineozzyozzyozzy
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Re: Is my WBS spawn bag too dry? [Re: This guy]
    #7612268 - 11/08/07 06:17 PM (16 years, 4 months ago)

Hmm... this is a little suspicious.

The front:



Note the excessive condensation [a recent thing] and the stringy rhizomorphic growth.

The back:



Which is very patchy and has parts where there is almost zero colonization, as with the top [and which I can only assume travels down throughout the rest of the spawn bag].

What are these symptoms of and how can I fix it so the bag colonizes evenly and quickly throughout all of the bag? Is the stringy growth and new condensation a sign that I should spawn it to coco coir prematurely?

[In terms of condensation, the weather has taken a turn for the wet and cold, instead of warm summer. It's inside my closed room inside a dark cupboard, but ambient temperature would still have dropped and humidity possibly risen.]

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Invisiblebudmanman
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Re: Is my WBS spawn bag too dry? [Re: ozzyozzyozzy]
    #7612333 - 11/08/07 06:42 PM (16 years, 4 months ago)

That sucks.


--------------------
Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal.

And I am mentally unstable.

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Offlinedrwatson
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Re: Is my WBS spawn bag too dry? [Re: budmanman]
    #7612388 - 11/08/07 07:06 PM (16 years, 4 months ago)

That doesn't look too good.:sad:
Dude I think you're going to have to toss that and try again.
Happens to everyone
:projectile:

Edited by drwatson (11/08/07 07:06 PM)

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Offlineozzyozzyozzy
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Re: Is my WBS spawn bag too dry? [Re: drwatson]
    #7612492 - 11/08/07 07:32 PM (16 years, 4 months ago)

Can I force a fruit now, just to test? I'll need a few more people saying "you're screwed" (and why, I have no idea what's gone wrong), but if I could expose to light and heat it up a little bit, fruiting invitro, I'd rather try that than just toss it.

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Offlinedrwatson
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Re: Is my WBS spawn bag too dry? [Re: ozzyozzyozzy]
    #7612844 - 11/08/07 09:10 PM (16 years, 4 months ago)

I'd bet you could still fruit it but you'd risk getting sick or having a bad trip if you eat them.

I think i've read that some people will still collect the spore prints to continue the strain to try again. This of course risks transporting the contam. as well though. Even if you cut away the bad parts the entire mass has been feeding with the same network so the risk of getting sick would still be there.

I don't think you can be 100% with this one but it looks like it might fruit if you let it.
Let's see what some of the more experienced members say.

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Offlineozzyozzyozzy
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Re: Is my WBS spawn bag too dry? [Re: drwatson]
    #7612902 - 11/08/07 09:26 PM (16 years, 4 months ago)

Hang on, why are we talking about contams? I haven't seen any, it's just a slow colonization because it's too wet, and the growth of the mycelium is putting out too much humidity, isn't it? Where's RR when you need him :P

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Offlineozzyozzyozzy
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Re: Is my WBS spawn bag too dry? [Re: ozzyozzyozzy]
    #7613193 - 11/08/07 11:05 PM (16 years, 4 months ago)

I looked closer and the WBS seeds had turned black on the back, so I think it definitely is a contam. I mixed it thoroughly so I'll dump it next to my desk and watch how it goes, but it's not very colonized so a contam and moisture problem definitely seems to be the solution. Time to knock up another bag I guess :frown:

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