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OfflinePr0_X
CultivationLifer
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Registered: 01/18/06
Posts: 617
Loc: Igloo's and polar bears
Last seen: 16 years, 1 day
Coffee, coffee and more coffee (2nd flush pics)
    #7557255 - 10/24/07 09:57 PM (16 years, 3 months ago)

Here are the initial pictures of 1:1 ratio rye/millet to water soaked coir and (diluted coffee) to coir.



^^^ Coffee soaked coir



^^^ Water soaked coir



This is the coffee soaked coir colonizing



This is the water soaked coir colonizing



Ive got some rye soaking in 2liters of water with 3/4 a cup of coffee, will be the next log i post.

So, the race is off, more pic's to come.

Not going to let my personal feelings overshadow the topic at hand. :smile:


--------------------
It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

- Support the FSR at www.fsrcanada.com and www.fsre.nl


Edited by Pr0_X (11/17/07 05:35 PM)


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Offlinerodfarva
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Registered: 07/31/07
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Re: Coffee, coffee and more coffee (Work in progress with pictures) [Re: Pr0_X]
    #7557275 - 10/24/07 10:01 PM (16 years, 3 months ago)

GREAT, btw what was the rumor?

Keep us updated:thumbup:


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OfflineVisionsToReality
RIBBONS


Registered: 09/22/07
Posts: 1,083
Last seen: 16 years, 1 month
Re: Coffee, coffee and more coffee (Work in progress with pictures) [Re: Pr0_X]
    #7557276 - 10/24/07 10:02 PM (16 years, 3 months ago)

Cool. You include the used coffee grounds too, right?


--------------------
Life is one big road with lots of signs,
So when you're ridin' through the ruts,
Don't you complicate your mind.

Flee from hate, mischief and jealousy
Don't bury your thoughts,
Put your vision to reality, yeah!


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OfflinePr0_X
CultivationLifer
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Registered: 01/18/06
Posts: 617
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Last seen: 16 years, 1 day
Re: Coffee, coffee and more coffee (Work in progress with pictures) [Re: VisionsToReality]
    #7557289 - 10/24/07 10:04 PM (16 years, 3 months ago)

No coffee grounds, just coir soaked in diluted black coffee...

Maxwell house original roast to be exact. It's still to early to really tell how well the coffee will out preform the water soaked coir but from here the mycelium on the coffee coir is recovering a hell of alot thicker then that of the coir soaked in water. Will keep updated.


--------------------
It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

- Support the FSR at www.fsrcanada.com and www.fsre.nl


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OfflineVisionsToReality
RIBBONS


Registered: 09/22/07
Posts: 1,083
Last seen: 16 years, 1 month
Re: Coffee, coffee and more coffee (Work in progress with pictures) [Re: Pr0_X]
    #7557311 - 10/24/07 10:10 PM (16 years, 3 months ago)

What's the point of doing coffee, again?


--------------------
Life is one big road with lots of signs,
So when you're ridin' through the ruts,
Don't you complicate your mind.

Flee from hate, mischief and jealousy
Don't bury your thoughts,
Put your vision to reality, yeah!


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OfflineTurntableJunky
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Registered: 04/26/07
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Re: Coffee, coffee and more coffee (Work in progress with pictures) [Re: VisionsToReality]
    #7557320 - 10/24/07 10:12 PM (16 years, 3 months ago)

Nitrogen.


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OfflineVisionsToReality
RIBBONS


Registered: 09/22/07
Posts: 1,083
Last seen: 16 years, 1 month
Re: Coffee, coffee and more coffee (Work in progress with pictures) [Re: TurntableJunky]
    #7557366 - 10/24/07 10:28 PM (16 years, 3 months ago)

Hmm...my gut tells me there's more protein and therefore nitrogen available in the coffee grounds than the brewed coffee itself. Brewed coffee = acidic...with coffee...and I thought tannins....not really protein or nitrogen.

But if it works, then I may be wrong...I know a lot of ppl do it. Anyone wanna chime in on this?


--------------------
Life is one big road with lots of signs,
So when you're ridin' through the ruts,
Don't you complicate your mind.

Flee from hate, mischief and jealousy
Don't bury your thoughts,
Put your vision to reality, yeah!


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OfflineRogerRabbitM
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Registered: 03/26/03
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Re: Coffee, coffee and more coffee (Work in progress with pictures) [Re: VisionsToReality]
    #7557603 - 10/25/07 12:11 AM (16 years, 3 months ago)

Quote:

In my time here at shroomery Ive done stupid things and have heard stupid things but if someone cant respect another's research stfu and move on to something else. Really fed up with some people.





wtf is that about?
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Offlinerodfarva
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Re: Coffee, coffee and more coffee (Work in progress with pictures) [Re: RogerRabbit]
    #7557620 - 10/25/07 12:22 AM (16 years, 3 months ago)

i wish i knew :dogpile:


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OfflineVisionsToReality
RIBBONS


Registered: 09/22/07
Posts: 1,083
Last seen: 16 years, 1 month
Re: Coffee, coffee and more coffee (Work in progress with pictures) [Re: RogerRabbit]
    #7557970 - 10/25/07 04:36 AM (16 years, 3 months ago)

RR I'm not sure why the hostility...
Edit: OOPS, misunderstood


Edited by VisionsToReality (10/25/07 08:27 AM)


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OfflinePr0_X
CultivationLifer
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Registered: 01/18/06
Posts: 617
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Last seen: 16 years, 1 day
Re: Coffee, coffee and more coffee (Work in progress with pictures) [Re: VisionsToReality]
    #7558093 - 10/25/07 06:22 AM (16 years, 3 months ago)

The pure black coffee as you said is acidic, raising the overall p.h of the coir to about the 7.2.7.8 margine hence it helps aid against contamination, also i'm testing the ability of useing black coffee for the tradtional well known nitrogen/akloid boost.
I forgot to add the coir is un-pasturized.

I am over tired and need to travel to a diffrent city in hopes of finding a real job lol, so when i come back i'll take a p.h test of the coir and let you all know exactly where it stands. Until then, take care.


--------------------
It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

- Support the FSR at www.fsrcanada.com and www.fsre.nl


Edited by Pr0_X (10/25/07 06:22 AM)


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OfflineRogerRabbitM
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Re: Coffee, coffee and more coffee (Work in progress with pictures) [Re: Pr0_X]
    #7558254 - 10/25/07 07:58 AM (16 years, 3 months ago)

Thanks for editing your first post.

Liquid coffee is slightly acidic, but coir is even more so. Coir runs pH 5 to pH 6.5 depending on origin. Acids lower pH, not raise them. I've found no evidence that coffee boosts potency. It does however, speed up colonization, because it's excellent fungi food. In addition, the lower pH results in faster growth because Cubensis prefers a substrate in the pH 5.5 to pH 6 range.

Using a substrate with an acidic pH like yours results in faster colonization and better performance overall, but also exposes the project to an increased risk of contamination, because molds such as trichoderma also prefer an acidic food source. In my area, the mold spore count is so high, I'd never be able to get a substrate that had been hydrated with liquid coffee colonized before parts of it were green from molds. Hopefully, yours will work out. It's almost colonized. Good luck.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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OfflineVisionsToReality
RIBBONS


Registered: 09/22/07
Posts: 1,083
Last seen: 16 years, 1 month
Re: Coffee, coffee and more coffee (Work in progress with pictures) [Re: RogerRabbit]
    #7558313 - 10/25/07 08:26 AM (16 years, 3 months ago)

K. So the main effect..of using liquid coffee is acidity..,..rather than nitrogen?

I get confused...fungus likes low pH...but then I read about adding lime to casing layers...etc...Why is the lime added, again? It's to raise the pH in the, for example, casing layer....so that the cubensis does not colonize it? Or is the pH raised to prevent contamination of the casing? Both? Kinda cloudy on this still.


--------------------
Life is one big road with lots of signs,
So when you're ridin' through the ruts,
Don't you complicate your mind.

Flee from hate, mischief and jealousy
Don't bury your thoughts,
Put your vision to reality, yeah!


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Invisiblemycocurious
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Registered: 02/09/07
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Re: Coffee, coffee and more coffee (Work in progress with pictures) [Re: VisionsToReality]
    #7558729 - 10/25/07 11:04 AM (16 years, 3 months ago)

Quote:

VisionsToReality said:
K. So the main effect..of using liquid coffee is acidity..,..rather than nitrogen?

I get confused...fungus likes low pH...but then I read about adding lime to casing layers...etc...Why is the lime added, again? It's to raise the pH in the, for example, casing layer....so that the cubensis does not colonize it? Or is the pH raised to prevent contamination of the casing? Both? Kinda cloudy on this still.




the casing layer is a non-nutritious contaminate barrier / humidity-blanket. By making it non-nutritious there is no food-source for contaminates to colonize and by raising the pH we make the environment hostile to their survival so the net result is an inhospitable moist blanket protecting the (relatively) vulnerable mycelium colony below.

Because the mycelium colony below is so well established and has a large food-source at it's disposal it can colonize through the slightly alkaline environment to reach the surface and fruit. In fact, I wouldn't be surprised if the process of colonizing that non-nutritious, slightly alkaline layer isn't stressful enough to be a fruiting trigger itself.

---
Or something like that... :sigh:


--------------------
:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

- How Myco-Curious Prepares Coir & Compost Substrates
- How Myco-Curious Builds A Bulk Humidifier
- How Myco-Curious Builds An Automated Greenhouse
------------------------------------
figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.


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OfflineVisionsToReality
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Registered: 09/22/07
Posts: 1,083
Last seen: 16 years, 1 month
Re: Coffee, coffee and more coffee (Work in progress with pictures) [Re: mycocurious]
    #7559252 - 10/25/07 01:31 PM (16 years, 3 months ago)

k thanks


--------------------
Life is one big road with lots of signs,
So when you're ridin' through the ruts,
Don't you complicate your mind.

Flee from hate, mischief and jealousy
Don't bury your thoughts,
Put your vision to reality, yeah!


Extras: Filter Print Post Top
OfflinePr0_X
CultivationLifer
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Registered: 01/18/06
Posts: 617
Loc: Igloo's and polar bears
Last seen: 16 years, 1 day
Re: Coffee, coffee and more coffee (Work in progress with pictures) [Re: VisionsToReality]
    #7570693 - 10/28/07 07:35 PM (16 years, 3 months ago)

[Update]

I cased them today with a 100% coir layer.

Both containers were runs in 5 days so to say liquid coffee boost the mycelium run is not right but it has supported stronger growth then that of the coir soaked only in water.

Here it was today before casing.



More pics to come.


--------------------
It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

- Support the FSR at www.fsrcanada.com and www.fsre.nl


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Invisiblejeetered
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Registered: 07/07/06
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Re: Coffee, coffee and more coffee (Work in progress with pictures) [Re: Pr0_X]
    #7570711 - 10/28/07 07:41 PM (16 years, 3 months ago)

looks good and healthy!


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OfflinePr0_X
CultivationLifer
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Registered: 01/18/06
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Re: Coffee, coffee and more coffee (Work in progress with pictures) [Re: jeetered]
    #7572169 - 10/29/07 07:47 AM (16 years, 3 months ago)

[Update]

Mycelium has begun penetrating the casing layer so I setup the fruiting chamber and threw in the casings,









Let's see how they fair up :smile:

More pics to come.


--------------------
It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

- Support the FSR at www.fsrcanada.com and www.fsre.nl


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OfflinePr0_X
CultivationLifer
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Registered: 01/18/06
Posts: 617
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Re: Coffee, coffee and more coffee (Work in progress with pictures) [Re: Pr0_X]
    #7602065 - 11/06/07 11:00 AM (16 years, 2 months ago)

[Bump]

Casing layer has been run and the coir coffee casing is showing much more fruit body production then the water soaked coir.

Here's a overview shot of them side by side in the FC.



and since my cam sucks here's some really blurry shots at the c/coir fruit bodies :smile:





Last night the pins you can see now were just primordia, it's only been 12 hours since I last looked at them :smile:

I'll update in a few days I guess.


--------------------
It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

- Support the FSR at www.fsrcanada.com and www.fsre.nl


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Invisiblejeetered
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Posts: 3,055
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Re: Coffee, coffee and more coffee (Work in progress with pictures) [Re: Pr0_X]
    #7602183 - 11/06/07 11:22 AM (16 years, 2 months ago)

i should have added spent coffee grounds to my invitro bags.


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OfflinePr0_X
CultivationLifer
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Re: Coffee, coffee and more coffee (Work in progress with pictures) [Re: jeetered]
    #7648458 - 11/17/07 06:11 PM (16 years, 2 months ago)

Sorry I didn't update for the first flush, seeing as that was the most important one of all....

In comparison with water soaked coffee to coffee soaked coir, the CCoir is much better on initial flushes I pulled a much higher wet weight off the coffee soaked coir then the water.

Here is them side by side after a 20 hour re hydration, 2nd flush is producing much, much larger fruits.

Total dry weight of the first was 14.7g's dry.

Less then what is expected but better something then nothing as-well as taking into consideration the spawn was treated in h2o2 after a wet spot outbreak in my jars. Tell me what you think.... it's only a 1cm substrate of pure coir spawned by 50/50 rye/millet.

Here they are six hours ago.




and most recent...



I had temperature problems during fruiting so keep in mind everything was less then optimal but at least it fruits....could have been worse :smile:


--------------------
It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

- Support the FSR at www.fsrcanada.com and www.fsre.nl


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Invisibletahoe
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Re: Coffee, coffee and more coffee (Work in progress with pictures) [Re: Pr0_X]
    #7648551 - 11/17/07 06:40 PM (16 years, 2 months ago)

the casing material is a little dry


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Invisiblekane.ish
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Re: Coffee, coffee and more coffee (Work in progress with pictures) [Re: tahoe]
    #7648587 - 11/17/07 06:53 PM (16 years, 2 months ago)

looks like the sides of your trays aren't covered...have you been having problems with side pinning at all?


--------------------
my cat knows my password, so it can be assumed that any and all posts I make are the result of the cat using the computer.

:tripping:


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OfflinePr0_X
CultivationLifer
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Re: Coffee, coffee and more coffee (Work in progress with pictures) [Re: kane.ish]
    #7648614 - 11/17/07 07:03 PM (16 years, 2 months ago)

side pinning galore, the foil I was useing fell off and I didn't bother covering it back up since it's on it's 2nd flush and not doing so well on coir alone... I could see better results with coffee grinds mixed in but diluted coffee with coir helps but not much.


--------------------
It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

- Support the FSR at www.fsrcanada.com and www.fsre.nl


Extras: Filter Print Post Top
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