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S1lverdj
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Registered: 07/22/06
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Jars Colonizing VERY slowly
#7548490 - 10/22/07 07:26 PM (16 years, 3 months ago) |
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So, first off, it's been a looong time since I had a grow. Anyways, I made 12 jars with verm/BRF and incubated 4 with Orissa India, 4 with John Allen, and 4 with Texas. I lost one to mold, but beyond that there is no sign of contams. However, I inoculated the jars on 09/17 and they've been kept in a TiT at around 84-86degrees since then. The jars still like a good half inch from the bottom and the entire bottom to be completely colozined. I'm wondering if it's the fact that I had had the syringes for over a year? Also, can this really slow colonization cause any other problems?
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505illation
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Re: Jars Colonizing VERY slowly [Re: S1lverdj]
#7548524 - 10/22/07 07:31 PM (16 years, 3 months ago) |
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A month is a very long time they should have been colonized by now. They are probably contamed. Maybe you need some more air exchange.
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yepyepyep
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Re: Jars Colonizing VERY slowly [Re: 505illation]
#7548539 - 10/22/07 07:34 PM (16 years, 3 months ago) |
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Is the substrate overly wet looking?
Did you pack the substrate?
Have you tried turning them upside down (to hopefully lose a bit of co2)?
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Pr0_X
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Re: Jars Colonizing VERY slowly [Re: yepyepyep]
#7549563 - 10/22/07 11:20 PM (16 years, 3 months ago) |
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Could be to high a water content to start with slowing growth followed by the cakes suffering dehydration later on, you need to remember the golden rule, the window of opportunity to completely colonize a jar is 25day's.... Anything more is not worth it.
If you knew what day of incubation the mold struck would be very helpful in pin pointing the issue. Mold shows up later in most cakes that have poor FAE, sooner in overly wet substrates with proper FAE or not at all, could be strictly a bacterial infection....
-------------------- It's okay to hurt my feelings cause you know, they're so numb anyway. but I guess it's what I get for being to fuckin stupid to stay away - Jake - Support the FSR at www.fsrcanada.com and www.fsre.nl
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veda_sticks
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Re: Jars Colonizing VERY slowly [Re: Pr0_X]
#7549937 - 10/23/07 04:47 AM (16 years, 3 months ago) |
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Temps are far too high, room temperature is fine, but if u really want optimal growing , 80 is just fine, higher than that your favouring the growth of contams rather than myc,
still a whole month is a long time. Try turning your jars upside down, its a longshot but its worth a try.
Incubating jars dont need FAE, for incubating your worring about gas exchange.
FAE is at fruting stage
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
Edited by veda_sticks (10/23/07 04:50 AM)
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Dave Bowman
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Re: Jars Colonizing VERY slowly [Re: veda_sticks]
#7549961 - 10/23/07 05:20 AM (16 years, 3 months ago) |
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Hate to Hijack, but is a month still a long time if it's a Full 1 Pint jar you are colonizing?
I just had 3 jars finish and its been 4 weeks this week and the other 3 are a little slower.
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yepyepyep
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Re: Jars Colonizing VERY slowly [Re: veda_sticks]
#7552459 - 10/23/07 07:37 PM (16 years, 3 months ago) |
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Quote:
veda_sticks said: Temps are far too high, room temperature is fine, but if u really want optimal growing , 80 is just fine, higher than that your favouring the growth of contams rather than myc,
Optimal growth is at the temperature range he is at.
http://www.shroomery.org/8484/What-are-the-optimum-P-cubensis-growth-parameters
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veda_sticks
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Re: Jars Colonizing VERY slowly [Re: yepyepyep]
#7553708 - 10/24/07 04:59 AM (16 years, 3 months ago) |
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The teks are needing updated,
RR will say the same as i am, 85 is too high.
Incubating at room temperature is fine, growing at 75 if your substrate is right should colinise in 12-14 days at 75.
I have had success incubating at 80,the only reason i dont incubate at 75 as my room temperature is a little lower than most peoples, around 60, and the cupboard i use is the water heater cubpoard and just happens to keep it aorund 80.
Quote:
86F is not optimal, no matter what. Mycelium growth in cubensis slows down at any temperature 83F and above. By 86, you're hurting the mushroom mycelium and stimulating thermophilic molds and bacteria. Normal room temperature works great for colonizing substrates, but at least try to keep it below 81F for best performance. RR
I have suggested temps of 85 and above being to high to a few people, only have have RR agree.
1 person i menchioned this to actually noticed an increase in growth since dropping from 86 to 80.
Also remember that mycelium will produce its own heat and result un being a few degrees warmer inside the jar than outside. If ur incubating at 85 your jar temp could be higher than 86.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
Edited by veda_sticks (10/24/07 05:09 AM)
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substraight
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Re: Jars Colonizing VERY slowly [Re: veda_sticks]
#7554112 - 10/24/07 08:40 AM (16 years, 3 months ago) |
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this guy I know was working around 85 it was going a bit slow (not too bad though), but the day after he backed it off to 80 or so he did notice a slight increase in colonization speed...I think. he's at about 70-80% at 11 days.
total noob with a small n cheap setup, he's still surprised he didn't have a contam problem.
just a side note...he's using 4oz (1/4 pint) jars (that's what I could get). anyone see any problem with that?
Edited by substraight (10/24/07 12:35 PM)
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RogerRabbit
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Re: Jars Colonizing VERY slowly [Re: substraight]
#7554142 - 10/24/07 08:49 AM (16 years, 3 months ago) |
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1/4 pint jars dry out too fast, and don't have enough substrate/moisture to sustain a decent flush. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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S1lverdj
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Registered: 07/22/06
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Re: Jars Colonizing VERY slowly [Re: RogerRabbit]
#7557332 - 10/24/07 10:16 PM (16 years, 3 months ago) |
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These jars are pint jars. I've flipped them upside down and everything. I can try lowering the temperature if they might help. There's no sign of mold whatsoever. I haven't noticed any bacterial infections either. However, the inside of the jars is rather damp. However, I used the same exact methods as I've used for around 6 or 7 grows now. Never had this problem before. It's really throwing me off.
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Pr0_X
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Re: Jars Colonizing VERY slowly [Re: S1lverdj]
#7557373 - 10/24/07 10:29 PM (16 years, 3 months ago) |
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sorry, not FAE. I ment poor gas exchange.
-------------------- It's okay to hurt my feelings cause you know, they're so numb anyway. but I guess it's what I get for being to fuckin stupid to stay away - Jake - Support the FSR at www.fsrcanada.com and www.fsre.nl
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veda_sticks
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Re: Jars Colonizing VERY slowly [Re: Pr0_X]
#7557978 - 10/25/07 04:40 AM (16 years, 3 months ago) |
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Pint jars will take slightly longer to colinise, so a full month isnt to bad, bring the temp down, this also vould be causing moisture to evaporate and condense on the sites of the glass, or it could be metobolites the myc is producing.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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S1lverdj
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Re: Jars Colonizing VERY slowly [Re: veda_sticks]
#7559684 - 10/25/07 03:21 PM (16 years, 3 months ago) |
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Thanks, I'll turn the temp down tonight and see what happens. I'm really concerned as that's just so much work down the drain. However, c'est la vie. I really appreciate all the feedback. If anybody else has ideas, keep em coming.
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veda_sticks
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Re: Jars Colonizing VERY slowly [Re: S1lverdj]
#7559820 - 10/25/07 04:12 PM (16 years, 3 months ago) |
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I had a 1/2 that took just over a month, lowering the temperature might help a bit, but if it still stalls, all is not lost, u can cut the uncolinised part of the cake off then dunk n roll as normal, yeilds wont be as good as if it fully colinised but at least you will get something.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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Mepher
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Re: Jars Colonizing VERY slowly [Re: veda_sticks]
#7561049 - 10/25/07 10:13 PM (16 years, 3 months ago) |
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I've seen pint jars that were incubated at poor conditions take nearly two months. These were forgotten, then put into more optimal conditions to finish, and ended up producing very well. So I would check your methods before growing again, S1lverdj, but do not give up on your jars just because they are slow.
Substrate may have been very well packed, or dried out. Did the cakes shrink much over this time? Turning upside-down may help them to finish, if bottom is uncolonized.
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