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stxlacrosse022
your localshroomer



Registered: 01/25/07
Posts: 333
Last seen: 15 years, 3 months
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lc syringe
#7547663 - 10/22/07 05:00 PM (16 years, 3 months ago) |
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I have a lc and i want to make a syringe out of it but dont have a clue what to do? Do i jsut put needle in the port i made and then suck up the myc and then eject it back into the lc and then inject some into syringe?
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 25 days
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You will want to sterilise your syringe
either by boiling water for around 20 minutes, sucking some up leaving it a minute, and reapeat a few times, then stick the syring in the port and suck up as much as u can get, give the lc a good swirl, u did put something in there to help break the mycelium apart??
though i think i preffer to pressure cook the syringe, draw in some water, wrap in tinfoil then PC at 15 psi for 20 minutes.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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pazzy
Always Hardcore


Registered: 08/24/07
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Last seen: 13 years, 2 months
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Take your sterilized empty syringe and flame the needle. Wipe it with alcohol to cool it down. Insert needle into liquid culture and fill it with the lc. Now use the L.C. to inoculate as if it were a normal spore syringe.
edit: beaten I have always boiled syringes to sterilize them since the syringes I have melt when pressure cooked. But pressure cooking is probably a better idea.
-------------------- Are you happy for a miracle?
Absolutely no source checks/discussions.
Edited by pazzy (10/22/07 05:11 PM)
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stxlacrosse022
your localshroomer



Registered: 01/25/07
Posts: 333
Last seen: 15 years, 3 months
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Re: lc syringe [Re: pazzy]
#7547778 - 10/22/07 05:21 PM (16 years, 3 months ago) |
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well i boiled the water for about 5-7 mins bc i was in a hurry but the temp was on high and it was at full boil once i checked so i think I'm gonna re-do the sterilization.Do i have to wait a day to use the syringe like i do with a usually new made one?
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NStoney
Stranger



Registered: 06/24/07
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Re: lc syringe [Re: pazzy]
#7547812 - 10/22/07 05:25 PM (16 years, 3 months ago) |
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When you say you have a LC, you are implying that there is allready mycelium in the liquid. Is that the case? Your post is a bit unclear..... i.e."inject some into syringe"
Typically the way it works is, first you create your LC. This is accomplished by placing spores, tissue, etc into a sterile nutrient solution in your container of choice. When the spores/tissue sufficiently colonizes the nutrient solution this is your LC.
Once you have a colonized LC, typically you would do as follows...
1) Steralize your syringe by either PCing it, or boil some water and draw it into your syringe & expel it somewhere besides back into your boiling water (Repeat the intake/expel boiling water process numerous times). 2) Steralize needle: flame, alcohol, etc 3) Insert needle into your LC & draw your mycelium solution into the syringe. 4) Remove the needle from the LC and cover the tip of the syringe -----prepare substrate to be inoculated------ 5) Re-steralize the tip of the needle: flame, alcohol, etc 6) Insert needle tip into your jars/bags of uncolonized substrate and inject some or all of the mycelium solution in the syringe. 7) Re-steralize needle tip (Flame/alcohol) between each injection
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stxlacrosse022
your localshroomer



Registered: 01/25/07
Posts: 333
Last seen: 15 years, 3 months
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Re: lc syringe [Re: NStoney]
#7547843 - 10/22/07 05:28 PM (16 years, 3 months ago) |
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yes i have already made an lc there is a thread on the page that says IS IT GOOD and thats my lc at the moment it was a b+ given to me by a VERY NICE AND GENEROUS PERSON ON HERE:) thx
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pazzy
Always Hardcore


Registered: 08/24/07
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Last seen: 13 years, 2 months
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You wait a day for spores to rehydrate. With LC you can inoculate immediately.
The way i sterilize my syringe is to get a sauce pan of water to a rolling boil. Fill your syringe and expunge it three times with the boiling water. Then fill it about 1/3 of the way up with rubbing alcohol and the rest with air and give it a good shake. Fill it with boiling water two more times to make sure the alcohol is out and it should be sterile.
It takes about 5 minutes and has served me well.
-------------------- Are you happy for a miracle?
Absolutely no source checks/discussions.
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stxlacrosse022
your localshroomer



Registered: 01/25/07
Posts: 333
Last seen: 15 years, 3 months
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Re: lc syringe [Re: pazzy]
#7547928 - 10/22/07 05:38 PM (16 years, 3 months ago) |
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thx will start incubating the new 6 pf jars tonight  old jars are around 40 percent done
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
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prepare to be amazed by lc. I really ought to start another.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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stxlacrosse022
your localshroomer



Registered: 01/25/07
Posts: 333
Last seen: 15 years, 3 months
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well i should have a ton of lc due to the fact i put in alot of spores
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
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I donnt think the amount of spores effect how much lc u get.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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stxlacrosse022
your localshroomer



Registered: 01/25/07
Posts: 333
Last seen: 15 years, 3 months
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o well shit lc is hard to suck up with a small needle and not enough water
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pazzy
Always Hardcore


Registered: 08/24/07
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Last seen: 13 years, 2 months
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There shouldnt be any water in the syringe until you suck up the LC. Try shaking it to break up the mycellium and tilting the jar so you can suck up more.
-------------------- Are you happy for a miracle?
Absolutely no source checks/discussions.
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wutang
fungi



Registered: 06/28/07
Posts: 1,903
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its actually rather easy unless your using those orange tipped insulin needles? forget that man
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stxlacrosse022
your localshroomer



Registered: 01/25/07
Posts: 333
Last seen: 15 years, 3 months
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Re: lc syringe [Re: wutang]
#7551260 - 10/23/07 03:01 PM (16 years, 3 months ago) |
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its a small needle thats why I'm having problems so i have to tilt it and the jar is a little big . but when i try to suck up the myc sometimes nothing will come inside the syringe? not even the liquid
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syntroniks
Stranger Danger
Registered: 10/18/07
Posts: 76
Loc: Assen, Holland
Last seen: 16 years, 1 month
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lol insulin.
Buy a spore syringe, drink the fluid and use one of those. Better, buy 2 so you'll have some spores left over after the drink.
it is possible to substitute inoculate for drink.
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pazzy
Always Hardcore


Registered: 08/24/07
Posts: 1,360
Last seen: 13 years, 2 months
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You could also get appropriate syringes at feed stores. Insulin needles are not good for mushroom culitivation.
-------------------- Are you happy for a miracle?
Absolutely no source checks/discussions.
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stxlacrosse022
your localshroomer



Registered: 01/25/07
Posts: 333
Last seen: 15 years, 3 months
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Re: lc syringe [Re: pazzy]
#7551906 - 10/23/07 05:30 PM (16 years, 3 months ago) |
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whats a good needle it has 3cc on the one im using
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 25 days
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You'll need a syringe with a bigger gauge needle, even then it sometimes blocks,
Thats why u usually put something like broken glass in your LC before it gets PC, so that every few days, u can shake the jar and the glass breaks it up.
oh guess what, i made an lc, and i forgot the bloody broken GLASS
BALLS
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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stxlacrosse022
your localshroomer



Registered: 01/25/07
Posts: 333
Last seen: 15 years, 3 months
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alright can i get a turkey needle yet?
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wutang
fungi



Registered: 06/28/07
Posts: 1,903
Last seen: 16 years, 2 months
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i don't think the glass shards are neccessary
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