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Offlinestxlacrosse022
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lc syringe
    #7547663 - 10/22/07 05:00 PM (16 years, 3 months ago)

I have a lc and i want to make a syringe out of it but dont have a clue what to do? Do i jsut put needle in the port i made and then suck up the myc and then eject it back into the lc and then inject some into syringe?


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Offlineveda_sticks
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Re: lc syringe [Re: stxlacrosse022]
    #7547693 - 10/22/07 05:06 PM (16 years, 3 months ago)

You will want to sterilise your syringe

either by boiling water for around 20 minutes, sucking some up leaving it a minute, and reapeat a few times, then stick the syring in the port and suck up as much as u can get, give the lc a good swirl, u did put something in there to help break the mycelium apart??

though i think i preffer to pressure cook the syringe, draw in some water, wrap in tinfoil then PC at 15 psi for 20 minutes.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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Offlinepazzy
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Re: lc syringe [Re: stxlacrosse022]
    #7547698 - 10/22/07 05:08 PM (16 years, 3 months ago)

Take your sterilized empty syringe and flame the needle. Wipe it with alcohol to cool it down. Insert needle into liquid culture and fill it with the lc. Now use the L.C. to inoculate as if it were a normal spore syringe.

edit: beaten
I have always boiled syringes to sterilize them since the syringes I have melt when pressure cooked. But pressure cooking is probably a better idea.


--------------------
Are you happy for a miracle?

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Edited by pazzy (10/22/07 05:11 PM)


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Offlinestxlacrosse022
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Re: lc syringe [Re: pazzy]
    #7547778 - 10/22/07 05:21 PM (16 years, 3 months ago)

well i boiled the water for about 5-7 mins bc i was in a hurry but the temp was on high and it was at full boil once i checked so i think I'm gonna re-do the sterilization.Do i have to wait a day to use the syringe like i do with a usually new made one?


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OfflineNStoney
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Re: lc syringe [Re: pazzy]
    #7547812 - 10/22/07 05:25 PM (16 years, 3 months ago)

When you say you have a LC, you are implying that there is allready mycelium in the liquid. Is that the case? Your post is a bit unclear..... i.e."inject some into syringe"

Typically the way it works is, first you create your LC. This is accomplished by placing spores, tissue, etc into a sterile nutrient solution in your container of choice. When the spores/tissue sufficiently colonizes the nutrient solution this is your LC.

Once you have a colonized LC, typically you would do as follows...

1) Steralize your syringe by either PCing it, or boil some water and draw it into your syringe & expel it somewhere besides back into your boiling water (Repeat the intake/expel boiling water process numerous times).
2) Steralize needle: flame, alcohol, etc
3) Insert needle into your LC & draw your mycelium solution into the syringe.
4) Remove the needle from the LC and cover the tip of the syringe
-----prepare substrate to be inoculated------
5) Re-steralize the tip of the needle: flame, alcohol, etc
6) Insert needle tip into your jars/bags of uncolonized substrate and inject some or all of the mycelium solution in the syringe.
7) Re-steralize needle tip (Flame/alcohol) between each injection


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Offlinestxlacrosse022
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Re: lc syringe [Re: NStoney]
    #7547843 - 10/22/07 05:28 PM (16 years, 3 months ago)

yes i have already made an lc there is a thread on the page that says IS IT GOOD and thats my lc at the moment it was a b+ given to me by a VERY NICE AND GENEROUS PERSON ON HERE:)
thx


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Offlinepazzy
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Re: lc syringe [Re: stxlacrosse022]
    #7547874 - 10/22/07 05:32 PM (16 years, 3 months ago)

You wait a day for spores to rehydrate. With LC you can inoculate immediately.

The way i sterilize my syringe is to get a sauce pan of water to a rolling boil. Fill your syringe and expunge it three times with the boiling water. Then fill it about 1/3 of the way up with rubbing alcohol and the rest with air and give it a good shake. Fill it with boiling water two more times to make sure the alcohol is out and it should be sterile.

It takes about 5 minutes and has served me well.


--------------------
Are you happy for a miracle?

:yinyang2:

Absolutely no source checks/discussions.


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Offlinestxlacrosse022
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Re: lc syringe [Re: pazzy]
    #7547928 - 10/22/07 05:38 PM (16 years, 3 months ago)

thx will start incubating the new 6 pf jars tonight :smile:
old jars are around 40 percent done


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Offlineveda_sticks
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Re: lc syringe [Re: stxlacrosse022]
    #7547950 - 10/22/07 05:43 PM (16 years, 3 months ago)

prepare to be amazed by lc. I really ought to start another.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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Offlinestxlacrosse022
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Re: lc syringe [Re: veda_sticks]
    #7548025 - 10/22/07 06:00 PM (16 years, 3 months ago)

:smile: well i should have a ton of lc due to the fact i put in alot of spores


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Offlineveda_sticks
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Re: lc syringe [Re: stxlacrosse022]
    #7548094 - 10/22/07 06:11 PM (16 years, 3 months ago)

I donnt think the amount of spores effect how much lc u get.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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Offlinestxlacrosse022
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Re: lc syringe [Re: veda_sticks]
    #7548871 - 10/22/07 08:41 PM (16 years, 3 months ago)

o well shit lc is hard to suck up with a small needle and not enough water :frown:


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Offlinepazzy
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Re: lc syringe [Re: stxlacrosse022]
    #7548926 - 10/22/07 08:50 PM (16 years, 3 months ago)

There shouldnt be any water in the syringe until you suck up the LC. Try shaking it to break up the mycellium and tilting the jar so you can suck up more.


--------------------
Are you happy for a miracle?

:yinyang2:

Absolutely no source checks/discussions.


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Offlinewutang
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Re: lc syringe [Re: stxlacrosse022]
    #7548927 - 10/22/07 08:50 PM (16 years, 3 months ago)

its actually rather easy
unless your using those orange tipped insulin needles? forget that man


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:peyotespectrum:    :sanpedro: :rainbowdrink: :tripping:


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Offlinestxlacrosse022
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Re: lc syringe [Re: wutang]
    #7551260 - 10/23/07 03:01 PM (16 years, 3 months ago)

its a small needle thats why I'm having problems so i have to tilt it and the jar is a little big :frown:. but when i try to suck up the myc sometimes nothing will come inside the syringe? not even the liquid


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Offlinesyntroniks
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Re: lc syringe [Re: stxlacrosse022]
    #7551279 - 10/23/07 03:05 PM (16 years, 3 months ago)

lol insulin.


Buy a spore syringe, drink the fluid and use one of those. Better, buy 2 so you'll have some spores left over after the drink.


it is possible to substitute inoculate for drink.


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Offlinepazzy
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Re: lc syringe [Re: syntroniks]
    #7551644 - 10/23/07 04:30 PM (16 years, 3 months ago)

You could also get appropriate syringes at feed stores. Insulin needles are not good for mushroom culitivation.


--------------------
Are you happy for a miracle?

:yinyang2:

Absolutely no source checks/discussions.


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Offlinestxlacrosse022
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Re: lc syringe [Re: pazzy]
    #7551906 - 10/23/07 05:30 PM (16 years, 3 months ago)

whats a good needle it has 3cc on the one im using


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Offlineveda_sticks
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Re: lc syringe [Re: stxlacrosse022]
    #7552059 - 10/23/07 06:08 PM (16 years, 3 months ago)

You'll need a syringe with a bigger gauge needle, even then it sometimes blocks,

Thats why u usually put something like broken glass in your LC before it gets PC, so that every few days, u can shake the jar and the glass breaks it up.

oh guess what, i made an lc, and i forgot the bloody broken GLASS

BALLS


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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Offlinestxlacrosse022
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Registered: 01/25/07
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Re: lc syringe [Re: veda_sticks]
    #7552067 - 10/23/07 06:10 PM (16 years, 3 months ago)

alright can i get a turkey needle yet?


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