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Invisiblebinarycircuit
Stranger

Registered: 10/03/07
Posts: 131
lc question/problem
    #7544901 - 10/21/07 10:14 PM (16 years, 3 months ago)

I made a potato dextrose LC in a pint jar. I found a tek that used two earplugs in the top for innoc and air exchange. Unfortunately I did not know that the jar shouldn't be filled up all the way.

After the LC was ready to draw from, I realized that there was way too much fluid to reach the myc so I drew out about 1/3 of the fluid. During this is when the problem happened. After going thru the earplug numerous times to drain some of the solution, the earplug popped through the hole in the lid and into the LC.

Not knowing what to do and figuring it would be contamed soon either way now that it was exposed, I quickly pulled the lid off completely, and drew out a very saturated 10ml of the myc cloud.

Do you think what I drew out is still be viable or is it pretty much fucked? I did this in open air, but the small room was doused with oust three times and everything was wiped with alchohol, I wore gloves and mask, ect...


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Invisibledysphoria
lost soul

Folding@home Statistics
Registered: 06/04/03
Posts: 1,651
Re: lc question/problem [Re: binarycircuit]
    #7544929 - 10/21/07 10:21 PM (16 years, 3 months ago)

chances are, assuming you pulled from under the surface of the water, and not directly from the surface water, you'll be ok. but with the earplug going into the solution and all, i dunno.

but you wont know until you inoculate a test jar, which i would definitely deem as necessary at this point to ascertain the viability of your LC, so you'll know whether to continue it, or ditch it and start anew.

ive used open air exposed LCs before, esp when trying to get the last syringe full of a jar, and most times, its worked without incident.


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OfflinePr0_X
CultivationLifer
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Registered: 01/18/06
Posts: 617
Loc: Igloo's and polar bears
Last seen: 16 years, 1 day
Re: lc question/problem [Re: dysphoria]
    #7544965 - 10/21/07 10:30 PM (16 years, 3 months ago)

Don't bother, your lc is lost just start over. you introduced a object that had been sitting over your lc protecting it from foreign spores and now it has landed inside, instant contamination. Sorry to be the burrer of bad news....

On the bright side, I think using a ear plug as a self healing injection port is a fabulous idea :smile:


--------------------
It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

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Offlinefallingaway24
catatonic selfmedicator

Registered: 12/25/04
Posts: 419
Last seen: 9 years, 6 days
Re: lc question/problem [Re: Pr0_X]
    #7545042 - 10/21/07 10:49 PM (16 years, 3 months ago)

whip up a couple half pint jars or something and give it a try


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"Most people don't know how they're gonna feel from one moment to the next. But a dope fiend has a pretty good idea. All you gotta do is look at the labels on the bottles." ~drugstore cowboy~


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Invisiblebinarycircuit
Stranger

Registered: 10/03/07
Posts: 131
Re: lc question/problem [Re: fallingaway24]
    #7545821 - 10/22/07 06:05 AM (16 years, 3 months ago)

Yeah I went ahead and used it... we'll see how it goes...

Fortunately the earplug was wiped with alchohol before it fell into the jar so maybe I will get lucky...


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Offlineveda_sticks
Cultivator
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Registered: 07/29/07
Posts: 14,191
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Re: lc question/problem [Re: binarycircuit]
    #7545834 - 10/22/07 06:13 AM (16 years, 3 months ago)

Chances are the 10ml you got might be fine.

You will jsut have to try.

My first lc i made from a shotglass, with tinfoil covering it, i had to remove the tinfoil to get to the myc and break it up with the syringe, i made a test jar and it worked.

To bad i only got enough lc for 1 jar.

The ear plug seems like a good idea, u could try glueing the earplug in with something next time to stop it popping through.

Im gonna try this as i have access to lots of earplugs and have a nice big jar suitable for LC.


--------------------

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Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
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Offlineboingkster
CuriousCultivator
Male

Registered: 09/28/07
Posts: 109
Last seen: 14 years, 1 month
Re: lc question/problem [Re: veda_sticks]
    #7545867 - 10/22/07 06:38 AM (16 years, 3 months ago)

Chances are with the mylecium being visible and hence dominant within the liquid culture jar, you'd be pretty safe if you drew from under the surface. Either way, going ahead and doing a jar [as you've decided to do] is a good idea. I mean, whats the worst that could happen, it'll go green? The best will be that you'll have perfectly colonised jars inside of 10 days. Sounds good to me :amanitajar:


--------------------
A Question Never Asked is an Answer Never Given
[Having said that, use the Search button! Teks too!]


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