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kane.ish
the ish


Registered: 10/20/07
Posts: 60
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rhizmorphic growth of casing
#7539463 - 10/20/07 02:18 PM (16 years, 3 months ago) |
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So a FOAF is unsure about what his first casing is doing.

9 BRF 1/2 pint cakes, PES Hawaiian strain cased with 50/50+. Used mostly agricultural limestone with a bit of hydrated lime, pH about 8.
1/2" bottom layer of 50/50, little over 1" deep substrate, then another 1/2" layer of 50/50.
The original colonization at 82F of the BRF jars was quick and extremely rhizmorphic, finishing in about 8 days after germination of spores.
After casing, the tray was placed in an incubator at about 82 F. After 1.5 days, the myc was poking through everywhere in little strands and the tray was placed in his FC with 95%+ RH, 12/12 light cycle and FAE every few hours, sometimes more (except at night). Temp is around 72 F. Misting done directly with an ultrasonic hum 2x daily (using a tube, piping it directly to the casing - very fine mist).
The pic was taken 4 days after initial casing, with only 2 days in FC. It looks like the vegetative rhizmorphic growth just won't stop and its just ripping through the casing layer. Ideas on how to push it into fruiting rather than myc growth? The pic isn't the best, but there are no solid blocks of myc on the surface, just lots of little strands poking through.
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orchidfanatic
retiree




Registered: 08/12/07
Posts: 832
Loc: where the wild things are
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Re: rhizmorphic growth of casing [Re: kane.ish]
#7539476 - 10/20/07 02:21 PM (16 years, 3 months ago) |
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just a few more days an it will pin .. that is what my trays look like now and pins are coming out by tens !
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Skeeblix
Dave Thomas



Registered: 01/28/07
Posts: 1,745
Loc: Wendy's
Last seen: 8 years, 3 months
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Re: rhizmorphic growth of casing [Re: orchidfanatic]
#7539495 - 10/20/07 02:27 PM (16 years, 3 months ago) |
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Hyphal knots all over that thing. This means pins are gonna form on all of those little protrusions. Looks like you got a nice pinset headed your way, too.
-------------------- This post approved by:
Premedman1 said:
I just shat my pants.
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veda_sticks
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Registered: 07/29/07
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Re: rhizmorphic growth of casing [Re: Skeeblix]
#7539932 - 10/20/07 04:36 PM (16 years, 3 months ago) |
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9 jars cased 50/50+, interesting would this give better results than just dunk and rolled cakes, in terms of total harvest? or would u just have a bigger but less flusshes
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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syntroniks
Stranger Danger
Registered: 10/18/07
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Re: rhizmorphic growth of casing [Re: veda_sticks]
#7539938 - 10/20/07 04:37 PM (16 years, 3 months ago) |
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kane.ish, welcome to the forums!
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kane.ish
the ish


Registered: 10/20/07
Posts: 60
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Re: rhizmorphic growth of casing [Re: syntroniks]
#7540005 - 10/20/07 05:01 PM (16 years, 3 months ago) |
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thanks for the welcome, I've read a decent amount of the forums here but just recently got around to registering.
I'm curious too to see how the BRF 50/50+ casing works out in terms of flushes/yield etc.
Just a waiting game at this point.
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kane.ish
the ish


Registered: 10/20/07
Posts: 60
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starting to get a lot of pins!
Done a bit of reading on the forums and still confused on whether or not to mist. Using an ultrasonic with an extremely fine mist, how often should the casing/pins be misted, if at all?
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Skeeblix
Dave Thomas



Registered: 01/28/07
Posts: 1,745
Loc: Wendy's
Last seen: 8 years, 3 months
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Re: misting [Re: kane.ish]
#7557832 - 10/25/07 02:09 AM (16 years, 3 months ago) |
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Was I right in thinking it was gonna be a nice pinset? Hook us up with some more porn, I wanna see it.
-------------------- This post approved by:
Premedman1 said:
I just shat my pants.
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kane.ish
the ish


Registered: 10/20/07
Posts: 60
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Re: misting [Re: Skeeblix]
#7558500 - 10/25/07 09:37 AM (16 years, 3 months ago) |
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I'll try to get some pin porn up once I can find my cable. All those little protrusions were not hyphal knots but seem to be just the myc growing through the casing layer and up into the air.
Anyway, a few days ago the myc growth seemed to stop and lose some of its vigor, and a day later there were little primordia popping up all over the place from underneath all that strandy surface mycelium. As far as I can tell, one side seems to be doing a bit better than the other, but still getting pins so yah, stay tuned for some pics.
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Skeeblix
Dave Thomas



Registered: 01/28/07
Posts: 1,745
Loc: Wendy's
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Re: misting [Re: kane.ish]
#7558829 - 10/25/07 11:27 AM (16 years, 3 months ago) |
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ah yeah, I see it now. it auto resized to fit my window, and since it's such a close shot I had trouble putting it into perspective. Either way, that's some good rhizo action you got going on.
Looking forward to seeing the results of your hard work.
-------------------- This post approved by:
Premedman1 said:
I just shat my pants.
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MotorCityMadman
Rubberfan andFunketeer



Registered: 10/02/06
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Re: misting [Re: Skeeblix]
#7559116 - 10/25/07 12:39 PM (16 years, 3 months ago) |
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primordia|pins => stop misting
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kane.ish
the ish


Registered: 10/20/07
Posts: 60
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yeah skeeblix, the cam is a piece anyway so its somewhat hard to make out details. Check out the pinset:

Like I said, not a very good camera and when I uploaded this it scaled it to 600x450 for some reason rather than keep the original 1600x1200. one side is definitely going crazy, the other has some activity but not as much, mostly on the edges.
I'm kind of concerned about the casing drying out a bit, and the myc is looking a little blue- don't want to abort all the pins with misting but w/o water not really sure how far they'll get. the casing layer itself is starting to pull away from the edges and pins are emerging there. what do you guys think of the situation? For the last day or two they have been getting 2 mists per day, and it doesnt seem to have stopped the pinning. Still getting new ones on the edges of the casing, but where it is drying its slowing down. Do all those pins really suck up all that water or does watering technique need improvement?
any way I can get the site to not scale my pics down when I upload? Still new to posting.
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BUDDHA_702
Master Mycologist In Training



Registered: 02/17/07
Posts: 1,296
Loc: Some Country
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Re: misting [Re: kane.ish]
#7560894 - 10/25/07 09:37 PM (16 years, 3 months ago) |
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Awesome pinset!!!
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spock1
Stranger


Registered: 04/14/07
Posts: 589
Last seen: 15 years, 7 months
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Re: misting *DELETED* [Re: kane.ish]
#7561042 - 10/25/07 10:11 PM (16 years, 3 months ago) |
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Post deleted by spock1Reason for deletion: .
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kane.ish
the ish


Registered: 10/20/07
Posts: 60
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Re: misting [Re: spock1]
#7561148 - 10/25/07 10:39 PM (16 years, 3 months ago) |
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Martha setup with a DC computer fan blowing in at about 3CFM. The ultrasonic is piped in at the top running constantly at its lowest setting. Because this still produces too much mist, there is a C-clamp on the hose so that with a few turns the amount of mist can be controled. Changing the %rH (measured with a decent electronic meter) is done by rotating the lever on the clamp in 1/4 turn increments one way or the other. The computer fan keeps the air pretty fresh in the FC, but FAE with a big fan is still done every few hours. The mist falls on a dripshield at the top so the mist isnt constantly on the casings.
To mist, the shield is taken away and the ultrasonic is pumped in at full blast for about 5 minutes with the DC fan off, dropping an extremely fine mist on the casing below.
So far misting doesn't seem to have hurt the pins, and they keep showing up so I'm inclined to say that its ok so long as the mist is really fine and there are no water droplets on the pins after misting. FOAF's first casing tho so still learning.
currently keeping it at about 85% rH
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spock1
Stranger


Registered: 04/14/07
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Last seen: 15 years, 7 months
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Re: misting *DELETED* [Re: kane.ish]
#7561196 - 10/25/07 10:51 PM (16 years, 3 months ago) |
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Post deleted by spock1Reason for deletion: .
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kane.ish
the ish


Registered: 10/20/07
Posts: 60
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Re: misting [Re: spock1]
#7561386 - 10/25/07 11:46 PM (16 years, 3 months ago) |
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the DC fan (60mm low speed) was picked up knowing it was a 3CFM. What size fans did you manage to dig up? Its hooked up to a rheostat so the voltage can be throttled down, but is typically run at full power (just turned down to almost nothing when misting).
1/2" ID hose runs from ultrasonic on the floor to FC, clamp is on the hose so when it gets constricted, the condensation formed will run back into the ultrasonic and get reused - works pretty well on keeping times between refills reasonable (3-4 days while running continuously).
the DC fan is mounted to blow directly in all the time (through a filter), but the FAEs are done by manually opening the martha and blowing directly in to make sure all the air is exchanged.
its a decent meter but not the best. i typically just look to the sides of the martha to tune the rh. a bunch of little microdots of condensation and a little bit of running drops down the sides, and that runs from about 80-95 on the meter. im sure its off a bit (or more), but so far just adjusting off the sides has worked so far
-------------------- my cat knows my password, so it can be assumed that any and all posts I make are the result of the cat using the computer.
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spock1
Stranger


Registered: 04/14/07
Posts: 589
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Re: misting *DELETED* [Re: kane.ish]
#7564567 - 10/26/07 08:06 PM (16 years, 3 months ago) |
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Post deleted by spock1Reason for deletion: .
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kane.ish
the ish


Registered: 10/20/07
Posts: 60
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Re: misting [Re: spock1]
#7566418 - 10/27/07 11:07 AM (16 years, 3 months ago) |
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not completely sure about the rheostat vs dimmer. The rheostat was found at a specialty computer shop in town, and just kinda does its thing.
I would imagine that the filter does create a loss of CFM, but the goal is mostly to just keep the ultrasonic fog at bay. Any extra FAE is nice too.
Had an option for using a higher CFM 120mm, but I figured with all that airflow the ultrasonic would have to run at a much higher setting to keep rH up and refilling it sucks, so in the end it was the 60. I would think you could get an almost constant good FAE with a 120mm, so long as you are able to keep rH up with your ultrasonic. hmmmm your thoughts?
good point on the rH measurement, how do you suggest to go about it?
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VisionsToReality
RIBBONS


Registered: 09/22/07
Posts: 1,083
Last seen: 16 years, 1 month
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Re: misting [Re: kane.ish]
#7566429 - 10/27/07 11:10 AM (16 years, 3 months ago) |
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When you say "FOAF" anyone could so easily argue that it DOESN'T necessarily mean what you want it to. Just had to say that.
-------------------- Life is one big road with lots of signs, So when you're ridin' through the ruts, Don't you complicate your mind. Flee from hate, mischief and jealousy Don't bury your thoughts, Put your vision to reality, yeah!
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spock1
Stranger


Registered: 04/14/07
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Re: misting *DELETED* [Re: kane.ish]
#7567122 - 10/27/07 03:47 PM (16 years, 3 months ago) |
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Post deleted by spock1Reason for deletion: .
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Funkatron9000
Astronaut



Registered: 10/03/07
Posts: 535
Loc: Sector Zed: Region 6: Sol...
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Re: misting [Re: spock1]
#7567572 - 10/27/07 06:33 PM (16 years, 3 months ago) |
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In my GH I have a US going every 4 hours for 5 minutes at a time and the RH is always 85% or higher. (takes 4 hours to dip down to 85 btw) and every now and again i will get acouple of aborts but all in all the pins/fuits do very well. just my 2 cents.
-------------------- You cannot yet touch my daughters awesome boobage
You must fight to the death in the Breastriary of Nippopolis.
I thought Gene Wilder was cool BEFORE he was dead.
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kane.ish
the ish


Registered: 10/20/07
Posts: 60
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Already started to notice the color of the casing layer as it relates to moisture content. Seems like a really good way to see how effective your rH and misting are doing.
Might look into getting a good mechanical hydro sometime, but for the time being I'll just do the best I can keeping the casing moist and rH up but not heavily condensing.
Quote:
In my GH I have a US going every 4 hours for 5 minutes at a time and the RH is always 85% or higher
funkatron, do you it set up on a timer or do it manually?
only timers I've seen are in 15 min increments
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spock1
Stranger


Registered: 04/14/07
Posts: 589
Last seen: 15 years, 7 months
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Re: misting *DELETED* [Re: kane.ish]
#7568148 - 10/27/07 10:08 PM (16 years, 3 months ago) |
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Post deleted by spock1Reason for deletion: .
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kane.ish
the ish


Registered: 10/20/07
Posts: 60
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Re: misting [Re: spock1]
#7570053 - 10/28/07 03:48 PM (16 years, 3 months ago) |
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Some pretty nice gear on that site, though finances right now are kinda preventing any big upgrades like that. Right now I just tend to run it at the lowest setting and give it as much FAE as possible.
I too have read somewhere that the cycling humidity helps trigger pinning. So far the misting/US on low combo seems to be working alright, most of the time only a few aborts but still having difficulty getting even pinsets.
-------------------- my cat knows my password, so it can be assumed that any and all posts I make are the result of the cat using the computer.
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kane.ish
the ish


Registered: 10/20/07
Posts: 60
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Re: misting [Re: kane.ish]
#7570106 - 10/28/07 04:02 PM (16 years, 3 months ago) |
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spock, any progress in seeing how in how running your ultrasonic at a lower setting affects casing moisure?
I'd just like to compare some notes
-------------------- my cat knows my password, so it can be assumed that any and all posts I make are the result of the cat using the computer.
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spock1
Stranger


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Re: misting *DELETED* [Re: kane.ish]
#7570348 - 10/28/07 05:08 PM (16 years, 3 months ago) |
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Post deleted by spock1Reason for deletion: .
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kane.ish
the ish


Registered: 10/20/07
Posts: 60
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Re: misting [Re: spock1]
#7571002 - 10/28/07 08:57 PM (16 years, 3 months ago) |
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what do you use the waxpaper for?
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Caribou_Lou
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Re: misting [Re: kane.ish]
#7571086 - 10/28/07 09:21 PM (16 years, 3 months ago) |
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do you see pins yet? I wanna see a picture when you do, I'm doing the same casings. How does 4.5 pints of BRF only make a 1" substrate though?
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