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Offlinetrippindad82
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Sadness
    #7538573 - 10/20/07 08:51 AM (16 years, 3 months ago)

AFOAF just lost his entire crop of cams this morning. The G2G transfers brought in other spores and although the jars are almost fully colonized, there are nice little spots of green and black wherever the jar isn't colonized. What a bummer. I guess the closet I had been using thus far for "G2G" transfers on my pf jars isn't as sterile as I had believed.


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Trying to explain a journey to someone who has never experienced it is like trying to explain what a zebra looks like to  blind person who has never seen a horse.

^^^The above matter may be a complete fantasy that I concocted out of possible boredom.^^^


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OfflineRogerRabbitM
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Re: Sadness [Re: trippindad82]
    #7538582 - 10/20/07 08:53 AM (16 years, 3 months ago)

Glovebox or flowhood. Never do open air inoculations or grain to grain transfers.
RR


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OfflinePr0_X
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Re: Sadness [Re: trippindad82]
    #7538588 - 10/20/07 08:55 AM (16 years, 3 months ago)

Grain to grain...

not grain to pf.... How would you mix up the peices of grain into a cake?
the idea of a G2G is to evenly displaced your colonized grains with the un-colonized, allows for more points of colonization, simply placing colonized rye grain ontop a pfcake just wont cutt it.


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It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

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Offlinetrippindad82
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Re: Sadness [Re: Pr0_X]
    #7538594 - 10/20/07 08:59 AM (16 years, 3 months ago)

Yeah, RR, I am definitely putting together a glovebox in a few days for my next grain transfer.

And Pr0_x, I would do tissue samples into the pf style jars. I never had any issues with them other than incredibly small harvests compared to what rye/bulk can do.


--------------------
Trying to explain a journey to someone who has never experienced it is like trying to explain what a zebra looks like to  blind person who has never seen a horse.

^^^The above matter may be a complete fantasy that I concocted out of possible boredom.^^^


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OfflineRogerRabbitM
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Re: Sadness [Re: Pr0_X]
    #7538598 - 10/20/07 09:03 AM (16 years, 3 months ago)

Quote:

simply placing colonized rye grain ontop a pfcake just wont cutt it.




Why not? I've done it many times. Of course, I use tyvek or filter disks instead of vermiculite. A few grain kernels on top of a pf jar will colonize it quickly.

I suspect he was using brf to brf, which is a lot trickier, especially in a closet. How would you get the verm barrier off, then break up the cake and spawn it to other cakes while maintaining sterility?
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Invisibleorchidfanatic
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Re: Sadness [Re: trippindad82]
    #7538600 - 10/20/07 09:04 AM (16 years, 3 months ago)

its easy to build a still air box. its the best spent time in this hobby I have ever spent. I have had only one contaminated petri dish so far out of 50 or so plates.. plus you can put any spore on a plate check for contamination isolate if needed then do agar to grain transfer and you will know your grain is contam free.
good luck


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Offlinetrippindad82
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Re: Sadness [Re: RogerRabbit]
    #7538880 - 10/20/07 10:51 AM (16 years, 3 months ago)

Quote:


I suspect he was using brf to brf, which is a lot trickier, especially in a closet. How would you get the verm barrier off, then break up the cake and spawn it to other cakes while maintaining sterility?




Yes I was using brf to brf. What I was doing was the following:

Prepared jars according to PF tek.
Sterilized the closet to the best of my ability.
Boiled a pair of tweezers and a scalpel.

I would open the master jar and scrape off the vermiculite. I would then use the scalpel and cut smallish pieces of colonized cake out and using the tweezers place two tissues samples on opposite sides of the new jar, just pushing below the contam barrier. I never once lost a jar to mold, although I did lose a couple to bacterial contam. However, I blame the suspect jars on poor sterilization.


My next grain to grain transfer will be done in my newly made glove box. Hopefully that will be done on thursday/friday as I already had a new multispore master jar on the way when I lost these ones. It's a bummer, but I learned my lesson.


--------------------
Trying to explain a journey to someone who has never experienced it is like trying to explain what a zebra looks like to  blind person who has never seen a horse.

^^^The above matter may be a complete fantasy that I concocted out of possible boredom.^^^


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OfflineCaribou_Lou
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Re: Sadness [Re: trippindad82]
    #7539007 - 10/20/07 11:41 AM (16 years, 3 months ago)

Yesterday I was about to inoculate 10 half pint jars so I was pulling back my cambodian syringe to get some air into it so I could shake it up and the thing came flying out of the syringe all the solution fell right onto my counter :frown:


Edited by Caribou_Lou (10/20/07 11:42 AM)


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Offlinesyntroniks
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Re: Sadness [Re: Caribou_Lou]
    #7539100 - 10/20/07 12:13 PM (16 years, 3 months ago)

Does tyvek breathe enough to let FAE but still keep moisture because I have some tyvek material around the house... That'd be better than plastic wrap 1000%


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Offlinetrippindad82
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Re: Sadness [Re: syntroniks]
    #7539500 - 10/20/07 02:29 PM (16 years, 3 months ago)

Quote:

syntroniks said:
Does tyvek breathe enough to let FAE but still keep moisture because I have some tyvek material around the house... That'd be better than plastic wrap 1000%




TYVEC as far as a filter medium on your lids? Yes, that will work. Plastic wrap doesn't breath which can stall the myc growth. TYVEC provides gas exchange and a contam barrier.


--------------------
Trying to explain a journey to someone who has never experienced it is like trying to explain what a zebra looks like to  blind person who has never seen a horse.

^^^The above matter may be a complete fantasy that I concocted out of possible boredom.^^^


--------------------------------------



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