|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
frankiedont
Stranger
Registered: 10/09/07
Posts: 3
Last seen: 16 years, 2 months
|
Questions from newbie for second try
#7538275 - 10/20/07 04:43 AM (16 years, 3 months ago) |
|
|
First of all.. what a great website! I wish I had known about it before my first attempt. In my first attempt I used the brown rice flour/vermiculite mix as a substrate, purchased my spore syringes from sporestore.com, used half-pint canning jars and sterilized by only boiling (instead of pressure cooker). The results after 3 weeks is that only 2 of 12 jars are growing mycellium. They are about half covered. I inoculated each jar in 4 places using 2 - 10cc syringes for the 12 jars, however, only one spot in each of the 2 jars that have mycellium growing started colonizing. I am thinking that there are several different reasons to my lack of success:
1) Didn't use a pressure cooker, boiled in pots for one hour instead. While there is yet to be any contamination, I am thinking that I may have evaporated off too much of the moisture in the substrate during this process.
2) Used tap water that was boiled for 15 minutes for the substrate mix. Even after boiling, is there a possibility of chlorine in the water?
3) the syringes I bought sucked.. in one of them I noticed a small clump of dark material (spores? or piece of mushroom debris from original print?). Perhaps I didn't shake them enough, although I did so quite vigorously.
I am going to let the 2 cakes continue.. they are about half covered. Should I shake them? They do have a vermiculite boundry layer at the top.
Questions for my next try:
1) Where is a good reliable source for spore syringes? I live in a state where they are legal to ship to.
2) Should I try a different substrate/method this time? Rye, birdseed, or stay with the brown rice flour and vermiculite?
3) I am thinking about using the mycology bags with filter patch.. or should I just use the jars again?
4) Is 'purified' (filtered) water bought from the grocery store a good source of water to mix the substrate? Is it sterile enough or should I boil it again to be safe?
Apologies for the long post.. Thanks in advance for suggestions from you experts out there..
Peace
|
monstermitch
Growing in Bags Doesn't Work



Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
|
Re: Questions from newbie for second try [Re: frankiedont]
#7538304 - 10/20/07 05:24 AM (16 years, 3 months ago) |
|
|
Quote:
frankiedont said: Questions for my next try:
1) Where is a good reliable source for spore syringes? I live in a state where they are legal to ship to.
2) Should I try a different substrate/method this time? Rye, birdseed, or stay with the brown rice flour and vermiculite?
3) I am thinking about using the mycology bags with filter patch.. or should I just use the jars again?
4) Is 'purified' (filtered) water bought from the grocery store a good source of water to mix the substrate? Is it sterile enough or should I boil it again to be safe?
Apologies for the long post.. Thanks in advance for suggestions from you experts out there..
Peace
1. www.sporeworks.com 2. personal choice. I think rye is very simple, maybe you should try. 3. personal choice. Depends on how much you'll use. 4. use any water you want. you'll be sterilizing your substrate in a pressure cooker anyway, so it's of no need to sterilize it ahead of time.
--------------------
|
yepyepyep
Stranger
Registered: 09/05/07
Posts: 147
Last seen: 26 days, 6 hours
|
Re: Questions from newbie for second try [Re: monstermitch]
#7538332 - 10/20/07 06:03 AM (16 years, 3 months ago) |
|
|
Unless you have now acquired a PC do not change substrate.
Above all buy from a decent sponsor here.. ralphsters/sporeworks/spores101 etc
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 367 topic views. 15 members, 191 guests and 37 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|