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Funkatron9000
Astronaut



Registered: 10/03/07
Posts: 535
Loc: Sector Zed: Region 6: Sol...
Last seen: 2 years, 2 months
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Problems with LC, any advice?
#7533182 - 10/18/07 09:18 PM (16 years, 3 months ago) |
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I seem to be having a problem with my LC.
I started out with a syringe of R44 LC, I inoculated 9 jars at 1cc each and used the remaining 1cc to make a fresh LC. The 9 jars took off great and quickly colonized their jars, the LC did just as well and quickly spread into enough LC to inoculate about 60 jars.
Sounds good so far right? Well I inoculated 1 test jar with some EQ LC and another with some R44 LC (whatever takes off first is what I am going to use)..... This was one week ago for the EQ jar and 5 days ago for the R44 jar and I have yet to see any growth in either 
Neither jar shows any signs of contam and they both smell exactly the same as the day after I cooked them. BUT, neither jar shows any sign of myc either... Soooooo I'm kinda getting alittle worried here. I know the best thing to do most the time is just wait and see what happens but has anyone ever had this happen? I mean the first jars hit with R44 LC showed myc at 3 days and was almost done in a week and a half.
Anyway let me know what you think please.
-------------------- You cannot yet touch my daughters awesome boobage
You must fight to the death in the Breastriary of Nippopolis.
I thought Gene Wilder was cool BEFORE he was dead.
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ozzyozzyozzy
Australian



Registered: 10/23/06
Posts: 545
Last seen: 3 years, 8 months
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Re: Problems with LC, any advice? [Re: Funkatron9000]
#7533358 - 10/18/07 10:01 PM (16 years, 3 months ago) |
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Did you inject right next to the side, so you can see the innoc point and where any growth might be?
Wait another week in case it's slow growth, in the meantime try using petri dishes to ensure the LCs aren't bung.
Edited by ozzyozzyozzy (10/18/07 10:02 PM)
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Funkatron9000
Astronaut



Registered: 10/03/07
Posts: 535
Loc: Sector Zed: Region 6: Sol...
Last seen: 2 years, 2 months
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Re: Problems with LC, any advice? [Re: ozzyozzyozzy]
#7535842 - 10/19/07 02:51 PM (16 years, 3 months ago) |
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Dont have any petri dishes atm I still need to go get some. And yea I injected down the side but then I slighty mix the grains abit just to help spread the growth when it happens. I'll go get some dishes soon from the doctors office and see waht happens.
-------------------- You cannot yet touch my daughters awesome boobage
You must fight to the death in the Breastriary of Nippopolis.
I thought Gene Wilder was cool BEFORE he was dead.
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