Home | Community | Message Board

MushroomCube.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Capsules   North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineoxohawkoxo
Myconaught
Male

Registered: 10/03/07
Posts: 23
Last seen: 12 years, 29 days
Grrr....
    #7532889 - 10/18/07 08:05 PM (16 years, 3 months ago)

Alright... So my first run was 7 jars BRF, they all got contaminated. Trych. Green mold. So I tried a second run, 6 jars, new syringe, different medium, same sterilization procedures and ... NOTHING ... literally, not even contaminates. So a third run, 5 jars with a BRF/WBS combo medium (had to use up the last of the BRF), a third syringe, and paranoid sterility. Cleaned the desk, the floor, my hands, flamed and alcohol between each jar plus worked very quickly. Now this is the most contaminated run of them all, actually it kinda looks like I inoculated them with spores for this blue/green mold; not the slightest sign of any mycelium.

Now I didn't use a still air box (I will next time). All 3 syringes were from a Shroomery vendor. ... Is it really this hard? Though I must admit I used to use metal lids with just coffee filters back in the day with limited success. I'm now using tyvek under metal plus tape(though not micropore, I'll get some of that stuff too) Two holes, one for injection and another for FAE.

Has anyone else had this level of trouble getting some spawn started?

Any crazy suggestions? Other than the micropore tape, and a still air box? I think I'm going to make my own syringes as well. I'm not sure I trust vendors...


Extras: Filter Print Post Top
OfflineChi Ro
Jive Ass Turkey
Male

Registered: 09/22/07
Posts: 211
Loc: Right hurr!
Last seen: 12 years, 5 months
Re: Grrr.... [Re: oxohawkoxo]
    #7532898 - 10/18/07 08:08 PM (16 years, 3 months ago)

It's really not that hard. My first two jars got contaminated and I have had no contaminations since.

...are you using a Pressure Cooker? You didn't mention it, and that would be the problem if you're just knocking up jars that haven't been sterilized.


--------------------


Extras: Filter Print Post Top
OfflineCtK
Stranger
Registered: 10/01/07
Posts: 31
Last seen: 12 years, 3 months
Re: Grrr.... [Re: oxohawkoxo]
    #7532924 - 10/18/07 08:13 PM (16 years, 3 months ago)

What do you mean, "Two holes, one for injection and another for FAE."

Are you leaving an open hole in your jars for FAE? I was under the impression colonizing jars didn't need FAE. If you have a completely open hole in all of your jars, it would probably explain the amount of contaminated jars you're producing.


Extras: Filter Print Post Top
InvisibleLayYouIn
Taurus
Male User Gallery


Registered: 09/28/06
Posts: 4,402
Loc: Organ
Re: Grrr.... [Re: oxohawkoxo]
    #7533003 - 10/18/07 08:31 PM (16 years, 3 months ago)

Quote:

oxohawkoxo said:
Any crazy suggestions? Other than the micropore tape, and a still air box? I think I'm going to make my own syringes as well. I'm not sure I trust vendors...




it's not the vendors.

where at in your jars do they start molding? is it in the spots you inoculated? if not, then you might not be sterilizing correctly.

what did you do with the jars that did nothing? ime, contaminates show up pretty fast(within 2-3 days). so, when i have no mold after the 4th day, i expect white mycelium and it's usually what i get.

Quote:

CtK said:
What do you mean, "Two holes, one for injection and another for FAE."

Are you leaving an open hole in your jars for FAE? I was under the impression colonizing jars didn't need FAE. If you have a completely open hole in all of your jars, it would probably explain the amount of contaminated jars you're producing.




the verm layer should act as a filter. i want to take the lid off of a jar after inoculating just to see what happens...


Extras: Filter Print Post Top
Offlineoxohawkoxo
Myconaught
Male

Registered: 10/03/07
Posts: 23
Last seen: 12 years, 29 days
Re: Grrr.... [Re: LayYouIn]
    #7533621 - 10/18/07 11:10 PM (16 years, 3 months ago)

Hi Guys,

Thanks for the responses. To answer a few questions, yes I have a pressure cooker. I generally put my jars (quart jars) in for an hour before taking them out. One of the jars that was contaminated in my first run was bought from a vendor pre-sterilized. I flamed and alcohol wiped immediately before inoculating that jar though.

In regards to the FAE I don't leave any holes in the lids. I make two holes in the metal caps, put tyvek underneath, and inoculate though one of the holes, then tape over it. The second hole is just so that I have enough gas exchange.

The jars that start molding at the sites where I've inoculated, which leads me to believe it's the syringes, or my sterilization methods. My last round are so severely contaminated that it looks as though I inoculated with spores for the blue mold that's in there. Though the holes are only open to the air for a few moments before I tape them over again. Hence why I think I may be in severe need of a still air box.

I'm in an apartment building and I'm not sure my vents even have filters on them. If a sterilized still air box would make up for the filters in the vents I'll go with the box, otherwise I could always look into a couple of filters.

The jars that did nothing, they're still sitting in a case untouched. They still have no sign of mycelium or contaminate. What are the odds of anti-fungals in a birdseed? My sterilization procedures for those jars is the very same as used for my first run and similar but less strict than used for the third and worst round of jars.

That's where it stands. I think the best steps are to get a still air box for inoculation, and maybe modify my lids?


Extras: Filter Print Post Top
OfflineThe shroomy 1
Luminous beings surround me
Male User Gallery


Folding@home Statistics
Registered: 03/27/07
Posts: 5,543
Loc: The Aether
Last seen: 5 months, 5 days
Trusted Cultivator
Re: Grrr.... [Re: oxohawkoxo]
    #7533675 - 10/18/07 11:39 PM (16 years, 3 months ago)

How funny, I always, always keep a "control" jar just for these reasons! I suggest everyone do the same. Don't inoculate one of the jars that have gone through the same procedures from start to finish. Hell, I have a blank syringe that gets pressure cooked and I use that to inoculate a jar. (I do that when I make my own syringes). The control jar is very important to me. Let's me know how sterile my procedures are, and makes isolating the contamination point so much easier. That's just me.

The shroomy 1


--------------------


AMU Q&A thread.


Extras: Filter Print Post Top
InvisibleRoachMan
Old Man
Male User Gallery


Folding@home Statistics
Registered: 03/06/05
Posts: 2,083
Loc: Midwest
Re: Grrr.... [Re: oxohawkoxo]
    #7533694 - 10/18/07 11:51 PM (16 years, 3 months ago)

Try putting the tyvek between the lid and ring.

(Not under the lid as this can allow the filter to become wet which allows contaminants to move through the layer.)


Extras: Filter Print Post Top
Offlineveda_sticks
Cultivator
Male User Gallery


Folding@home Statistics
Registered: 07/29/07
Posts: 14,191
Loc: UK Flag
Last seen: 4 years, 25 days
Re: Grrr.... [Re: RoachMan]
    #7534053 - 10/19/07 03:49 AM (16 years, 3 months ago)

Make yourself a glove box, when i first started i didnt have a glove box, my first run i had 2 jars that didnt grow, then the next batch 3 out of 4 contam, then the next batch 1 out of 3. Then i made a glove box, 2 jars nicely colinising.

Most syringes come with the needle seperate, the syringe could have got contamed in the process of removing the the cap and isntalling the needle.

Make yourself a glovebox, and this will eliminate a possibility.

Mould in your jars means that they are probably getting contamed at inoc or incubating.

You could also try disinfecting your incubating area, i have mine in a nice warm cupboard, i give that a spray with beach everynow and then.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


Extras: Filter Print Post Top
InvisibleThePyschonaut52
Stranger
Male

Registered: 04/24/07
Posts: 982
Re: Grrr.... [Re: veda_sticks]
    #7534173 - 10/19/07 05:27 AM (16 years, 3 months ago)

from my experience, most contaminates enter the syringe when you put the needle on. You need a glove box.


--------------------
"In god we trust..."


-I guess we're screwed.


Extras: Filter Print Post Top
InvisibleRoachMan
Old Man
Male User Gallery


Folding@home Statistics
Registered: 03/06/05
Posts: 2,083
Loc: Midwest
Re: Grrr.... [Re: veda_sticks]
    #7534589 - 10/19/07 09:18 AM (16 years, 3 months ago)

Quote:

veda_sticks said:
Make yourself a glovebox, and this will eliminate a possibility.





Eliminate a possibility, yes, however, I've done 100's of open air inoculations without a contamination.

(Not that I've never had a contaminant, but my only contaminants were back when I was a noobie and didn't pressure cook my grains long enough.)


Extras: Filter Print Post Top
Invisiblesimplemachine
Manfly
Male User Gallery

Registered: 09/14/03
Posts: 1,981
Re: Grrr.... [Re: oxohawkoxo]
    #7534668 - 10/19/07 09:46 AM (16 years, 3 months ago)

Quote:

The jars that start molding at the sites where I've inoculated, which leads me to believe it's the syringes





I think you are right on this one. Your syringes should definitely be made in a still air box. Inoculating in there isn't as important, because its easy to clean whats OUTSIDE the needle, but you have be make a clean syringe first.


--------------------
     


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Capsules   North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* inoculation procedures, in open air. Tippinthru 4,146 8 08/08/06 04:00 PM
by Tippinthru
* Fruiting contaminated cake. sexymoreno 3,929 9 08/21/07 07:41 AM
by orchidfanatic
* Contaminated Syringe? Comfortably_Numb 1,300 3 12/07/03 03:27 PM
by Comfortably_Numb
* One contamination after another downforpot 881 8 11/21/04 03:46 PM
by scatmanrav
* Quick cloning procedure overview--all right? GnuBobo 1,098 5 04/25/05 08:48 PM
by GnuBobo
* jar to jar contamination sub_simple 777 2 11/30/04 04:15 PM
by lemon_lw
* Contamination, very minor area - still a major problem? HerbanShaman 830 2 04/10/05 09:53 PM
by HerbanShaman
* Contamination Probabilities Akira 1,142 15 01/12/06 10:09 AM
by Akira

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
435 topic views. 16 members, 169 guests and 37 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.011 seconds on 14 queries.