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Pr0_X
CultivationLifer



Registered: 01/18/06
Posts: 617
Loc: Igloo's and polar bears
Last seen: 16 years, 1 day
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Australian p.cubensis on rye+millet (RR, Agar, Creamcorn, experts! pls help)
#7530807 - 10/18/07 10:55 AM (16 years, 3 months ago) |
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Ok, Ive already made a previous post about this, slow colonization of the rye/millet, it's been 16 day's and the jar's are less then 25% colonized. I followed everything to the T every tid bit of information i got from rye teks was from here.

I bought four packages of rye and a bag of millet, I placed the grains in two large pots and added water, letting it skim the grains of excess starch and debris while I mixed it until the water was 100% clear of debris (I used tap water). I then let them sit for 24 hours and soak, the next day I rinsed the rye and millet seperatly and extremely well before boiling the rye for 20 minutes (until i seen some burst kernels) I then mixed up the rye in millet in a large pot and added it to a collender to drain for a hour before placing them in 12 1 litre mason canning jar's.
I then added the lids wich were prepared the day before useing a nickle sized hole about 1/2inch in width/length, I placed polyfil through the holes, added the lid, a sheet of tyvek from a tyvek suit and placed on the ring as tight as possible.
I then placed the jar's in my 22quart mirror Preasure cooker leaving the weight off to allow all excess air inside to escape (waiting to see a steady flow of steam before placing on the preasure regulator, I counted down 1 and a half hour's from time of the first weight jiggle, removed the pc from the stove and let it de-preasurize in the bathtub...
I removed the jar's and sat them still wrapped in tinfoil on the kitchen table and allowed to cool prior to innoculating with 3cc's of spore solution, as the topic says I innoc'd with a Australian print I received from Anand (thanks again man) I should also add I prepared the syringes the day before to allow the spores to rehydrate for 24 hours.
So, I inoculated in open air (sprayed the room down with lysol 5 minutes prior) and set the jar's in the incubator set to 28c. It took three days before I noticed the initial signs of growth but now its going onto 16 days and from previous experience it only takes 25days to completely finish a 1litre jar but my jar's are less then half way colonized on day 16, I am getting really frustrated see'ing as everything was done right and proper, it was a multispore inoculation but still, they are just going way to slow and slowly succumbing to bactilius..
The growth itself is really weird aswell, the one jar has a straight line of mycellium going right around it but its not growing up or down...
So should I just toss these fucking jars and start all over again? I really want to give them a chance and I do have patience but a month and a half (estimated) is abit to long for a jar... all 12 jars are obviously diffrent substrains so its not a genetic issue...
I need some experts to put there heads together and tell me what is up, sorry if i come off as being pissed but it's understandable its $30 worth of grains and alot of my time and effort. Thanks in advance for any help and here's a picture of the jars all set up.

-------------------- It's okay to hurt my feelings cause you know, they're so numb anyway. but I guess it's what I get for being to fuckin stupid to stay away - Jake - Support the FSR at www.fsrcanada.com and www.fsre.nl
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: Australian p.cubensis on rye+millet (RR, Agar, Creamcorn, experts! pls help) [Re: Pr0_X] 1
#7530859 - 10/18/07 11:12 AM (16 years, 3 months ago) |
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I can't imagine what tek would have you do that many things wrong. Here's a few of the errors.
You boiled the rye, but not the millet? Then mixed the two? That gives you two different grains with different levels of hydration.
Draining for an hour does nothing. All the water that will drain out from a batch of rye does so within 1 minute. The water that is stuck to the surface of the grains, will make them too wet later. You need to drain after the simmer while the grains are still boiling hot, so you can let the steam dry on the surface.
Avoid busted kernels at all costs. None is best. If you have more than just a few, do the batch over. If you've soaked, beginning in HOT tap water, there should be no busted kernels after boiling because they will have softened up.
Did you leave the stove on high during the process? If so, your jars probably puked out all the moisture that was in the grains. You're supposed to turn down the stove as soon as the weight rattles. Turn it down so the weight either doesn't rattle at all, or rattles once every few minutes at most. Every time the weight rattles, moisture leaves the PC, and a corresponding amount of moisture leaves each jar of grains. That is basic physics.
Polyfill is a decent filter, as is tyvek. Using both will restrict gas exchange.
You removed the PC from the stove and brought down the pressure in the bathtub??? That sped up the cooling process, causing even more loss of moisture from the grains. You're supposed to leave the PC on the stove and simply shut off the burner and walk away. NEVER do anything to speed up the pressure loss.
You DON'T inoculate in open air. In addition, Lysol is a surface cleaner, not an air cleaner. Lysol will do nothing to clean the air.
I'm sure with some reading, you can find more mistakes. Once you correct these, and follow proper procedure, I'm sure you'll have success. Good luck. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Pr0_X
CultivationLifer



Registered: 01/18/06
Posts: 617
Loc: Igloo's and polar bears
Last seen: 16 years, 1 day
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Re: Australian p.cubensis on rye+millet (RR, Agar, Creamcorn, experts! pls help) [Re: RogerRabbit]
#7530987 - 10/18/07 11:54 AM (16 years, 3 months ago) |
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When I simmered the grains I did so by bringing the pot of water to a boil, added the rye and turned down the heat to medium/low, immiedatly after I removed the pot from the stove I placed the contents in a collender and shook them until all excess water dripped off/evaporated and then let the moisture on the outside of the kernels dry off (I thought this was the proper procedure) I forgot to mention, I only soaked the millet because of its small size it tends to burst fairly quickly when simmered and the soak alone is normally enough to hydrate it to 99.99% of its capacity, i tryed simmering it a few times before but just ended up with a bunch of bursted kernels, its somewhat hard to catch.
During the PC after the weight was jiggling I adjusted the tempature so that the weight jiggled every few minutes.
Are the jars going to be ok? should I expect the mycellium to take longer then normal because of the restricted FAE and botched moisture content? am I answering my own question?
Thank you very much RR, your answers are much appreicated by my friends and I
-------------------- It's okay to hurt my feelings cause you know, they're so numb anyway. but I guess it's what I get for being to fuckin stupid to stay away - Jake - Support the FSR at www.fsrcanada.com and www.fsre.nl
Edited by Pr0_X (10/18/07 12:00 PM)
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jeetered
Stranger



Registered: 07/07/06
Posts: 3,055
Loc: no clue
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Re: Australian p.cubensis on rye+millet (RR, Agar, Creamcorn, experts! pls help) [Re: Pr0_X]
#7531036 - 10/18/07 12:08 PM (16 years, 3 months ago) |
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hmm, i've inoculated everything for the past 3 years in open air, and have gotten less contams, then when i used a flowhood/glovebox.
other then that, i agree with everything RR said.
Pr0_X
LOL u used to have a handle on this what up buddy? been that long!!!!
GET BACK TO WORK
<3
u better get your sh** together brother
j/k goodluck man.
I honestly think your only problem is the escape of all the moisture, never ever speed up cooling your PC, it takes like 10 hours then you're ready to use. I always let mine sit overnight, because i do it so my PC is finished right before i go to bed, so when i wake up in the morning, i can go straight to inoculating, sometimes it's still a little warm, so i Shi* shower and shave first, then eat, then it's pretty much ready.
Edited by jeetered (10/18/07 12:10 PM)
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Pr0_X
CultivationLifer



Registered: 01/18/06
Posts: 617
Loc: Igloo's and polar bears
Last seen: 16 years, 1 day
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Re: Australian p.cubensis on rye+millet (RR, Agar, Creamcorn, experts! pls help) [Re: jeetered]
#7531154 - 10/18/07 12:52 PM (16 years, 3 months ago) |
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Haha, I know Jeet, been smokin to much ganja inbetween my cultivation break I think, slowed the mind and further stupified me.
I was never to good with grain's mainly because I'd always remove the PC from the stove after a hour and a half and let it cool down for a hour or so before sitting it in a tub of luke warm water....
I should be able to bring down my jar colonization time by atleast two weeks now, Thank you so much RR
Now its time to go get myself some more grains and try it RR's way.
-------------------- It's okay to hurt my feelings cause you know, they're so numb anyway. but I guess it's what I get for being to fuckin stupid to stay away - Jake - Support the FSR at www.fsrcanada.com and www.fsre.nl
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: Australian p.cubensis on rye+millet (RR, Agar, Creamcorn, experts! pls help) [Re: Pr0_X]
#7531260 - 10/18/07 01:29 PM (16 years, 3 months ago) |
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Quote:
When I simmered the grains I did so by bringing the pot of water to a boil, added the rye
That is probably the problem. This destroys the grains, often causing the kernels to burst open. ALWAYS set the grains in the water they soaked in on the stove, and then turn on the burner. This heats the grains slowly so they don't ruin. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Captain Cubensis
Bleeding HeartLiberal


Registered: 09/18/07
Posts: 648
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Re: Australian p.cubensis on rye+millet (RR, Agar, Creamcorn, experts! pls help) [Re: RogerRabbit]
#7531645 - 10/18/07 03:04 PM (16 years, 3 months ago) |
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Stale air with a little Oust works fine for my foaf, no flow hood.
He says it's more about a clean needle and speed, or spoon if doing G2G.
To encourage air flow around your area creates a vacuuming that any and all contams in the low pressure zone will flow into and around the ENTIRE area.
I never understood flow hoods, except that it is ion keeping with the overly elaborate who like to make it sound like rocket science.
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