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Invisiblebandog
Stranger


Registered: 01/19/07
Posts: 109
Not sure ...
    #7530061 - 10/18/07 04:37 AM (16 years, 3 months ago)

... but I think this might be some contamination in the corner of one of my boxes ...



Just kidding about this, of course ... it's definitely contamination.

This is one box of six - bulk (colonized brown rice - spawned to coir/verm/wormpoo/coffee grounds/gypsum - cased with verm)
To avoid contamination such as this, I've been advised:
1. to fan more frequently ("trich doesn't like CO2")
2. to switch to a mono-tub tek

Of the above, which will be more effective in avoiding these types of contaminations?


--------------------
Come in to my parlour, said the spider to the fly ...


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InvisibleBUDDHA_702
Master Mycologist In Training
Male


Registered: 02/17/07
Posts: 1,296
Loc: Some Country
Re: Not sure ... [Re: bandog]
    #7530069 - 10/18/07 04:40 AM (16 years, 3 months ago)

look like it to me, but I'm color blind.


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OfflineRogerRabbitM
Bans for Pleasure
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
Trusted Cultivator
OG Cultivator
Re: Not sure ... [Re: BUDDHA_702]
    #7530257 - 10/18/07 06:44 AM (16 years, 3 months ago)

The key to avoiding molds is sterile inoculations, filtered gas exchange during colonization, and lots of air exchange during fruiting.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Invisiblebandog
Stranger


Registered: 01/19/07
Posts: 109
Re: Not sure ... [Re: RogerRabbit]
    #7536054 - 10/19/07 03:46 PM (16 years, 3 months ago)

Thanks RR :wink:

Is it just my imagination, or has the theory of what
causes trich contamination changed over the past six
months or so :confused:...

I tossed my contaminated substrate photos up on all
the mushroom forums, asking for suggestions as to how
I could avoid the nasty green bastard in future.
Without exception, every response was to the effect of
" ... the key to avoiding/eliminating trich contamination
in bulk grows, is to increase the PH of the substrate ... "
followed by everyone's favourite method of accomplishing
that - i.e., oyster shells, gypsum, lime, etc

This time, no matter where I post the photo of the latest
trich contamination, asking for suggestions as to how I
can better avoid the green death, every person, without
exception, suggests increasing FAE :confused:
So ... has the theory changed??


--------------------
Come in to my parlour, said the spider to the fly ...


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OfflineRogerRabbitM
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: Not sure ... [Re: bandog]
    #7536108 - 10/19/07 04:02 PM (16 years, 3 months ago)

No, the theory hasn't changed. People are simply still confused as usual. You don't need to pH balance substrates, since they fully colonize within ten days to two weeks. Mushroom mycelium prefers an acidic substrate, but is more tolerant of a high pH than molds, thus we raise the pH on our casing layers since they don't fully colonize.

If your substrate contaminated, chances are your spawn was contaminated. If your casing layer contaminated, increase fresh air exchange. From the looks of what I see above, I'd suspect your problem originated at inoculation, or perhaps you didn't wash away all of the vermiculite filter from your cakes before spawning to bulk. Remember, the tops of your jars are nasty if you're using a verm filter. Often, trichoderma or other molds will germinate and actually be growing in the verm filter, especially if you turned the jars upside down, which I don't recommend. Be sure to rinse the verm off under the faucet before spawning to bulk.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Invisiblebandog
Stranger


Registered: 01/19/07
Posts: 109
Re: Not sure ... [Re: RogerRabbit]
    #7536180 - 10/19/07 04:22 PM (16 years, 3 months ago)

Excellent points - and excellent advice - thanks again, RR!

How probable is it for trich to actually be introduced to and
contaminate a fully colonized tray of substrate with or without
casing?

Just for reference, I didn't use a verm barrier during
incubation ... which might also have left the rice more
open and prone to contamination ... right?


--------------------
Come in to my parlour, said the spider to the fly ...


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OfflineRogerRabbitM
Bans for Pleasure
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
Trusted Cultivator
OG Cultivator
Re: Not sure ... [Re: bandog]
    #7536474 - 10/19/07 05:29 PM (16 years, 3 months ago)

Yes, and that's why it's contaminated. You have to use filters.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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