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OfflinePr0_X
CultivationLifer
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Registered: 01/18/06
Posts: 617
Loc: Igloo's and polar bears
Last seen: 13 years, 8 months
Liquid culture dust particles
    #7528592 - 10/17/07 07:08 PM (14 years, 7 days ago)

Ive begun a liquid culture useing 2tbs of dextrose to 2cups of water (473ml) give or take a ml or so...

I heated the water prior to mixing in with the dextrose and then filtered the mix six times through triple layered coffee filters, I then pc'd at 15psi for 20 minutes and allowed the jars to cool, i'm all ready to inoculate with A-strain but just now I noticed some small dust particles floating around in the LC that I couldnt see before, perhaps the steam from the water hid them somehow. Eitherway im at a total loss for words, I wanted it 100% clear with no dust particles... Either way, before I go and waste 2cc's of spore solution on these jar's I wanted to know if these dust particles will cause contamination.

I'm useing some discarded pickle jar's for the LC's and I cleaned them with soap/water then rinsed them out to avoid just what I got, dust particles!!!! rargh.

Am i ok to inoculate? RogerRabbit I could use your advice.


--------------------
It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

- Support the FSR at www.fsrcanada.com and www.fsre.nl


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InvisibleThePyschonaut52
Stranger
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Registered: 04/24/07
Posts: 982
Re: Liquid culture dust particles [Re: Pr0_X]
    #7528653 - 10/17/07 07:26 PM (14 years, 7 days ago)

if your worried, just make another batch.


--------------------
"In god we trust..."


-I guess we're screwed.


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OfflinePr0_X
CultivationLifer
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Registered: 01/18/06
Posts: 617
Loc: Igloo's and polar bears
Last seen: 13 years, 8 months
Re: Liquid culture dust particles [Re: ThePyschonaut52]
    #7528677 - 10/17/07 07:33 PM (14 years, 7 days ago)

Every LC I ever made has had a few dust particles in it but that was when I first started out and had many failures due to a lack of knowledge on the subject of liquid culture's, now I'm useing agar's lid design with the tube's and keeping the polyfill dry with the proper amount of nutrient media....

No matter how well I clean the jars they always have floating dust particles in them but this is the first time I know everything is done to the T i'm just wondering if the few dust particles that are there will pose a problem.


--------------------
It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

- Support the FSR at www.fsrcanada.com and www.fsre.nl


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OfflineCheesekiller
Mad Scientist
Male


Registered: 11/08/04
Posts: 438
Loc: Central NY
Last seen: 13 hours, 20 minutes
Re: Liquid culture dust particles [Re: Pr0_X]
    #7528773 - 10/17/07 08:03 PM (14 years, 7 days ago)

I had them in mine, but I just figured they were sterilized, so I bumped my jars with the stuff anyway. You can see my pic of my LC on the First LC thread.

You should be alright.

-CK


--------------------
Bulk grower with "some" success.

  Cloning Machine 
   
Nice Lids

A few pics of my DT setup


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