Home | Community | Message Board

Sporeworks
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds High THC Strains   North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflinePr0_X
CultivationLifer
Male User Gallery


Registered: 01/18/06
Posts: 617
Loc: Igloo's and polar bears
Last seen: 16 years, 1 day
Liquid culture dust particles
    #7528592 - 10/17/07 05:08 PM (16 years, 3 months ago)

Ive begun a liquid culture useing 2tbs of dextrose to 2cups of water (473ml) give or take a ml or so...

I heated the water prior to mixing in with the dextrose and then filtered the mix six times through triple layered coffee filters, I then pc'd at 15psi for 20 minutes and allowed the jars to cool, i'm all ready to inoculate with A-strain but just now I noticed some small dust particles floating around in the LC that I couldnt see before, perhaps the steam from the water hid them somehow. Eitherway im at a total loss for words, I wanted it 100% clear with no dust particles... Either way, before I go and waste 2cc's of spore solution on these jar's I wanted to know if these dust particles will cause contamination.

I'm useing some discarded pickle jar's for the LC's and I cleaned them with soap/water then rinsed them out to avoid just what I got, dust particles!!!! rargh.

Am i ok to inoculate? RogerRabbit I could use your advice.


--------------------
It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

- Support the FSR at www.fsrcanada.com and www.fsre.nl


Extras: Filter Print Post Top
InvisibleThePyschonaut52
Stranger
Male

Registered: 04/24/07
Posts: 982
Re: Liquid culture dust particles [Re: Pr0_X]
    #7528653 - 10/17/07 05:26 PM (16 years, 3 months ago)

if your worried, just make another batch.


--------------------
"In god we trust..."


-I guess we're screwed.


Extras: Filter Print Post Top
OfflinePr0_X
CultivationLifer
Male User Gallery


Registered: 01/18/06
Posts: 617
Loc: Igloo's and polar bears
Last seen: 16 years, 1 day
Re: Liquid culture dust particles [Re: ThePyschonaut52]
    #7528677 - 10/17/07 05:33 PM (16 years, 3 months ago)

Every LC I ever made has had a few dust particles in it but that was when I first started out and had many failures due to a lack of knowledge on the subject of liquid culture's, now I'm useing agar's lid design with the tube's and keeping the polyfill dry with the proper amount of nutrient media....

No matter how well I clean the jars they always have floating dust particles in them but this is the first time I know everything is done to the T i'm just wondering if the few dust particles that are there will pose a problem.


--------------------
It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

- Support the FSR at www.fsrcanada.com and www.fsre.nl


Extras: Filter Print Post Top
OfflineCheesekiller
Mad Scientist
Male


Registered: 11/08/04
Posts: 485
Loc: Central NY
Last seen: 1 month, 1 day
Re: Liquid culture dust particles [Re: Pr0_X]
    #7528773 - 10/17/07 06:03 PM (16 years, 3 months ago)

I had them in mine, but I just figured they were sterilized, so I bumped my jars with the stuff anyway. You can see my pic of my LC on the First LC thread.

You should be alright.

-CK


--------------------
Bulk grower with "some" success.

  Cloning Machine 
   
Nice Lids

A few pics of my DT setup


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds High THC Strains   North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* Spores, G2G, Liquid Culture, Cloning & Colonization Time.
( 1 2 3 all )
agar 52,763 43 07/08/23 07:04 AM
by Screwup
* Liquid Culture Challenges Leimana 9,559 4 05/31/05 11:33 PM
by Corporal Kielbasa
* 1st Liquid Culture, arrrgh! Seril 14,292 10 02/28/06 08:35 PM
by Limelight_Liver
* Liquid Culture Recipes MrMaddHatter 23,904 13 03/29/05 04:53 AM
by Sbealzebub
* how to use cardboard cultures to inoculate grain jars and how to use a liquid culture to inoculate lordoftheshroomz 6,509 12 05/08/05 03:55 PM
by lordoftheshroomz
* Clear liquid cultures ? mickywilliams2 3,665 11 10/12/05 05:02 PM
by Bamaman
* i can get liquid cultures, but not grain laughingbuddha 2,807 8 03/08/05 12:02 PM
by laughingbuddha
* eating mycelium from liquid culture
( 1 2 all )
lepiota 11,085 31 06/02/05 07:14 AM
by Dexter_Morgan

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,003 topic views. 18 members, 156 guests and 57 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.02 seconds spending 0.008 seconds on 14 queries.