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RoachMan
Old Man




Registered: 03/06/05
Posts: 2,083
Loc: Midwest
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Mycelium Water Example
#7521325 - 10/15/07 10:10 PM (16 years, 3 months ago) |
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EDIT in (i): Sterile water injected into jar on the left, extracted, and injected into the two jars on the right.
(10/08)
(Open-Air)
Edited by RoachMan (10/15/07 11:00 PM)
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TheHauntingSoul
Not really that cool



Registered: 03/15/05
Posts: 810
Loc: Ottawa, Canada
Last seen: 13 years, 9 months
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Re: Mycelium Water Example [Re: RoachMan]
#7521635 - 10/15/07 11:11 PM (16 years, 3 months ago) |
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i dont get it... elaborate
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meatcakeman
the search for bodhisattva



Registered: 07/03/07
Posts: 8,380
Loc: el sol
Last seen: 10 years, 9 months
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Re: Mycelium Water Example [Re: RoachMan]
#7521648 - 10/15/07 11:18 PM (16 years, 3 months ago) |
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so you colonized the other jars this way? or ... what?
-------------------- 大开眼界
 
Hasta siempre, comandante.
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RoachMan
Old Man




Registered: 03/06/05
Posts: 2,083
Loc: Midwest
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Re: Mycelium Water Example [Re: meatcakeman]
#7521660 - 10/15/07 11:23 PM (16 years, 3 months ago) |
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Water was drawn up into a syringe, wrapped with aluminum foil, steamed it for 25 minutes, let cool, shook the jar on left, injected the water into the jar on left, withdrew the water with the same syringe, and injected that water into the two jars on the right.
That was on 10/08 using NO glovebox.
Other jars? You mean...from the other thread? NO...those were LC
You can get quality spawn using many methods.
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meatcakeman
the search for bodhisattva



Registered: 07/03/07
Posts: 8,380
Loc: el sol
Last seen: 10 years, 9 months
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Re: Mycelium Water Example [Re: RoachMan]
#7521663 - 10/15/07 11:25 PM (16 years, 3 months ago) |
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thanks for clarifying
your first post had me flabbergasted
*yeah, i said flabbergasted.
-------------------- 大开眼界
 
Hasta siempre, comandante.
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RoachMan
Old Man




Registered: 03/06/05
Posts: 2,083
Loc: Midwest
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Re: Mycelium Water Example [Re: meatcakeman]
#7521666 - 10/15/07 11:26 PM (16 years, 3 months ago) |
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I'm not certain why, but no problem.
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meatcakeman
the search for bodhisattva



Registered: 07/03/07
Posts: 8,380
Loc: el sol
Last seen: 10 years, 9 months
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Re: Mycelium Water Example [Re: RoachMan]
#7521671 - 10/15/07 11:27 PM (16 years, 3 months ago) |
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to be honest i reread your post again and i have no idea why either... haha i probably read something wrong or looked something over
-------------------- 大开眼界
 
Hasta siempre, comandante.
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FreeSporePrints


Registered: 03/06/05
Posts: 1,139
Loc: Rome, Italy
Last seen: 1 month, 26 days
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Re: Mycelium Water Example [Re: RoachMan]
#7521776 - 10/16/07 12:06 AM (16 years, 3 months ago) |
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Interesting test..but for every 2 jars colonized you must sacrifice (shaking) one.
I believe it's better proceed commonly with agar wedge and save your time sterilizing twice.
Fabio
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RoachMan
Old Man




Registered: 03/06/05
Posts: 2,083
Loc: Midwest
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You don't sacrifice the jar; you can still use it as spawn.
Sterilizing twice?
The syringe once, and the rye jars.
This is not about agar...this is about mycelium water.
Someone with no agar skills will appreciate this more than you.
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btb103
Stranger


Registered: 02/17/07
Posts: 229
Last seen: 10 years, 8 months
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Re: Mycelium Water Example [Re: RoachMan]
#7521975 - 10/16/07 02:00 AM (16 years, 3 months ago) |
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Very cool! A sort of g2g transfer by using only water. Did you let the sterilized water sit in the colonized "mother jar" for a time or remove it immediately?
EDIT: How long did colonization take for the other jars?
-------------------- In bewilderment, I realized I am the endless sea.
Edited by btb103 (10/16/07 02:01 AM)
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Shastro
Stranger

Registered: 08/23/07
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Last seen: 14 years, 29 days
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Re: Mycelium Water Example [Re: btb103]
#7522013 - 10/16/07 02:30 AM (16 years, 3 months ago) |
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cool, seems quick and easy. could we get a writeup
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FreeSporePrints


Registered: 03/06/05
Posts: 1,139
Loc: Rome, Italy
Last seen: 1 month, 26 days
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Re: Mycelium Water Example [Re: RoachMan]
#7522048 - 10/16/07 03:17 AM (16 years, 3 months ago) |
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Hello RoachMan.
First of all i want to specify that that's my opinion and i said it's interesting, but for me every 2 jars of spawn you sacrifice 1 other jar (that spawn i will not use for future grain to grain transfers if i have some large batch to inocule).
Growing mushrooms requests knowledge, costancy and passion. Yes growing Psilocybe cubensis is extremely easy, and your tek is really useful, but imho contamination rate using your tek isn't an unnegligible fact. Grains, as you know, are a really rich food for bacteria. You can't know if in your spawn there are dormant bacterias that will blow into the uncolonized grain (except if you check the heath of the mycelium with a microscope..at 400x if i don't remember bad.
IMHO people must know and be able to manage cultures, before trying every existent tek. I believe a glovebox is a thing that everyone at 99% can afford.
Have a nice day, Fabio
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toadstooly
searcher oftruth



Registered: 07/13/07
Posts: 252
Loc: where the wild things are...
Last seen: 15 years, 11 months
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I recently did the same thing.
I took the jar that donated the myc. and spawned it to coir in a small shoebox sized container. It's still incubating but so far so good. Of course, i let the jar re-colinize before spawning it. But the jars i used the grain lc on are colinizing very quickly. They'll be 100% in right around 1 week after knocking them up.
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mebesideme
o-bliminal


Registered: 08/19/04
Posts: 177
Last seen: 7 years, 1 month
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Re: Mycelium Water Example [Re: toadstooly]
#7522202 - 10/16/07 07:09 AM (16 years, 3 months ago) |
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This is what my friend's doing now! He has a 60cc syringe he will fill with water to use, per another tek he found here. Eager to see results that good!
-------------------- Doot de dooh.... yep yep yep. A signature and stuff...
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RoachMan
Old Man




Registered: 03/06/05
Posts: 2,083
Loc: Midwest
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Contamination is NEVER "negligible".
However, healthy spawn is just that...HEALTHY.
You said "dormant bacteria" in the spawn. That is why the rye jars are heat/pressure sterilized...to kill competing fungi and bacteria.
Using liquid mycelium is the same as doing G2G (as far as results go).
This tek is mainly directed at people who do not have a glovebox, can't afford to build one at the moment, or just don't want another big rubbermaid container lying around.
It's also meant for people who may not have experience making LC, have had contaminations using LC, and who may not have skills with doing agar work.
It's simple, effective, takes much less time to expand your mycelium than waiting on LC to grow, etc.
Personally, I PREFER using LC, and I just did this because RogerRabbit always talks about how liquid Mycelium is better than LC.
It is (per RR) a better way to expand mycelium because it is less contamination prone than an LC.
I hope this clears some things up; I'll try to do a write-up today for Shastro.
LC and liquid mycelium grow at about the same rate, both kinds of jars colonize in 7-10 days (on rye). (Using no incubator, room temperature, and stored in drawer.) A lot of times it grows so fast I don't even bother shaking until it is time to spawn them.
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