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jeetered
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Registered: 07/07/06
Posts: 3,055
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Jeetereds Easy Liquid Culture Tek *DELETED*

#7519318 - 10/15/07 12:39 PM (16 years, 6 months ago)

Post deleted by jeetered

Reason for deletion: my own reasons

Edited by jeetered (10/19/07 08:51 PM)

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ohmatic
searcher

Registered: 02/28/04
Posts: 6,742
Loc: europe

Re: Jeetereds Easy Liquid Culture Tek

[Re: jeetered]
#7519363 - 10/15/07 12:53 PM (16 years, 6 months ago)

meh ...

j/k :thumbup:

:thumbup:

--------------------
MONOTUB tek HEATBOMB tek

RIP #cultivation! ....can't associate? well FUCK U !

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Asante
Omnicyclion prophet

Registered: 02/06/02
Posts: 87,330

Re: Jeetereds Easy Liquid Culture Tek

[Re: jeetered]
#7519379 - 10/15/07 12:59 PM (16 years, 6 months ago)

Quote:

Notice it looks like Semen floating in Urine

At which point does the cow shit come in? :lol:

:lol:

Looks good, the procedure!

--------------------
Omnicyclion.org
higher knowledge starts here

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blood4blood
Calmer Than You Are

Registered: 04/25/07
Posts: 6,029
Loc: The Valley

Re: Jeetereds Easy Liquid Culture Tek

[Re: Asante]
#7519410 - 10/15/07 01:06 PM (16 years, 6 months ago)

nice one jeetered. how many cc's of spore solution did you add to the medium?

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bmy
Stranger to myself

Registered: 09/19/07
Posts: 222
Loc: Planet Earth
Last seen: 14 years, 2 months

Re: Jeetereds Easy Liquid Culture Tek

[Re: ohmatic]
#7519425 - 10/15/07 01:10 PM (16 years, 6 months ago)

What is the purpose of the polyfil in the syringe?

When you input fresh air via the syringe, do you fill up the vacuum? If not - wont the outside pressure be able to push air through the punctured silicone?

What is the purpose of using two kinds of syrups?

Sorry for all the questions :smile:

:smile:

Nice writeup :thumbup:

:thumbup:

--------------------

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jeetered
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Re: Jeetereds Easy Liquid Culture Tek *DELETED*

[Re: blood4blood]
#7519441 - 10/15/07 01:15 PM (16 years, 6 months ago)

Post deleted by jeetered

Reason for deletion: ytf

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jeetered
Stranger

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Re: Jeetereds Easy Liquid Culture Tek

[Re: bmy]
#7519450 - 10/15/07 01:18 PM (16 years, 6 months ago)

Quote:

bmy said:
What is the purpose of the polyfil in the syringe?

When you input fresh air via the syringe, do you fill up the vacuum? If not - wont the outside pressure be able to push air through the punctured silicone?

What is the purpose of using two kinds of syrups?

Sorry for all the questions Nice writeup

I use both for good reason, a) pans dont like karo alone b) both cause quick colonization c) it's how i do things YO.

I dont let off all the vacuum no, i leave it slightly under pressure, keeps contams out.

the silicone is self healing, and no, it doesnt leak. The polyfil acts as a filtration medium

Edited by jeetered (10/15/07 01:23 PM)

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bmy
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Re: Jeetereds Easy Liquid Culture Tek

[Re: jeetered]
#7519526 - 10/15/07 01:39 PM (16 years, 6 months ago)

How does under pressured jars keep contams out? I would figure it would make it easier for stuff to get in. Over pressured on the other hand...

Sorry for sounding as if I don't trust your teks, that's not the case. I'm just a curious being. Knowledge is power! :smile:

:smile:

--------------------

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blood4blood
Calmer Than You Are

Registered: 04/25/07
Posts: 6,029
Loc: The Valley

Re: Jeetereds Easy Liquid Culture Tek

[Re: bmy]
#7519534 - 10/15/07 01:42 PM (16 years, 6 months ago)

its not "under pressured" its underpressure. that means there is pressure being exerted on the medium.

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jeetered
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Re: Jeetereds Easy Liquid Culture Tek

[Re: bmy]
#7519543 - 10/15/07 01:44 PM (16 years, 6 months ago)

man, take it as it is, It works, I have never had a contam doing it this way.

Edited by jeetered (10/16/07 05:04 PM)

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Pr0_X
CultivationLifer

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Re: Jeetereds Easy Liquid Culture Tek

[Re: bmy]
#7519553 - 10/15/07 01:46 PM (16 years, 6 months ago)

Nice write up Jeet, you obviously put alot of time into it by the pictures very nice for the beginners here looking to get into LC's :smile:

:smile:

All the people who find Jeet's tek a informitive beginner friendly tek should give the man 5 shroomies, less he comes to your house with his tomahawk :tongue:

:tongue:

--------------------
It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

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bmy
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Re: Jeetereds Easy Liquid Culture Tek

[Re: jeetered]
#7519567 - 10/15/07 01:48 PM (16 years, 6 months ago)

Take it easy *hides behind the sofa*. As I said I trust it to work very well. I was just curious about the technicalities behind it.

--------------------

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VisionsToReality
RIBBONS

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Re: Jeetereds Easy Liquid Culture Tek

[Re: bmy]
#7519651 - 10/15/07 02:14 PM (16 years, 6 months ago)

You close the lids tightly before pressure cooking? Wouldn't that be a hazard?

--------------------
Life is one big road with lots of signs,
So when you're ridin' through the ruts,
Don't you complicate your mind.

Flee from hate, mischief and jealousy
Don't bury your thoughts,
Put your vision to reality, yeah!

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ThePyschonaut52
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Re: Jeetereds Easy Liquid Culture Tek

[Re: VisionsToReality]
#7520013 - 10/15/07 03:41 PM (16 years, 6 months ago)

Very nice tek jeetered. I'm bookmarking this one :thumbup:

:thumbup:

--------------------
"In god we trust..."

-I guess we're screwed.

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orchidfanatic
retiree

Registered: 08/12/07
Posts: 832
Loc: where the wild things are

Re: Jeetereds Easy Liquid Culture Tek

[Re: VisionsToReality]
#7520094 - 10/15/07 04:05 PM (16 years, 6 months ago)

Quote:

VisionsToReality said:
You close the lids tightly before pressure cooking? Wouldn't that be a hazard?

only to the inside of the PC ..
when I make a LC with rubber plugs as inj sites .. they form a vacuum
a needle with polyfil will vent it to normal air . curious on the "pressure" part myself..

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jeetered
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Re: Jeetereds Easy Liquid Culture Tek *DELETED*

[Re: VisionsToReality]
#7520358 - 10/15/07 05:43 PM (16 years, 6 months ago)

Post deleted by jeetered

Reason for deletion: fgh

Edited by jeetered (10/15/07 05:44 PM)

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roquet
Expat tippler

Registered: 05/29/07
Posts: 1,195
Loc: Dubai بجدية عربي...

Re: Jeetereds Easy Liquid Culture Tek

[Re: jeetered]
#7520771 - 10/15/07 07:16 PM (16 years, 6 months ago)

Quote:

jeetered said:
jeetered wrote:
will need about 60cc's of air until it's fully colonized.

if this is the case, I'm wondering if it wouldn't be easier to PC the jars with loose lids and tighten them as soon as you open the PC, meaning you don't have a vacuum so no need to inject sterile air. Would there be a big risk of contams getting in in the time between opening the PC and tightening the lids? If not, are there advantages to having a vacuum and controlling the amount of air added?

Have people found vacuum LCs with no added air don't work at all? I'm still finding out by trial and error. Mine are only small (200ml liquid, 100ml vacuum space).

I'm not totally sure how a vacuum works. Presumably there is *some* oxygen in a vacuum at the top of a jar or else the sides would cave in? Just less than normal. And there is oxygen in the water.

Sorry to go a bit off topic. Nice write-up, Jeetered :thumbup:

:thumbup:

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roquet
Expat tippler

Registered: 05/29/07
Posts: 1,195
Loc: Dubai بجدية عربي...

Re: Jeetereds Easy Liquid Culture Tek

[Re: jeetered]
#7520796 - 10/15/07 07:24 PM (16 years, 6 months ago)

Quote:

jeetered said:
jeetered wrote:
will need about 60cc's of air until it's fully colonized.

if this is the case, I'm wondering if it wouldn't be easier to PC the jars with loose lids and tighten them as soon as you open the PC, meaning you don't have a vacuum so no need to inject sterile air. Would there be a big risk of contams getting in in the time between opening the PC and tightening the lids? If not, are there advantages to having a vacuum and controlling the amount of air added?

Have people found vacuum LCs with no added air don't work at all? I'm still finding out by trial and error. Mine are only small (200ml liquid, 100ml vacuum space).

I'm not totally sure how a vacuum works. Presumably there is *some* oxygen in a vacuum at the top of a jar or else the sides would cave in? Just less than normal. And there is oxygen in the water.

Sorry to go a bit off topic. Nice write-up, Jeetered :thumbup:

:thumbup:

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roquet
Expat tippler

Registered: 05/29/07
Posts: 1,195
Loc: Dubai بجدية عربي...

Re: Jeetereds Easy Liquid Culture Tek

[Re: jeetered]
#7520864 - 10/15/07 08:09 PM (16 years, 6 months ago)

Quote:

jeetered said:
will need about 60cc's of air until it's fully colonized.

if this is the case, I'm wondering if it wouldn't be easier to PC the jars with loose lids and tighten them as soon as the PC is opened, meaning they don't have a vacuum so no need to add air. Would there be a big risk of contams getting in in the time between opening the PC and tightening the lids? If not, are there other advantages to having a vacuum and controlling the amount of air added?

Have people found vacuum LCs with no added air don't work at all? I'm still finding out by trial and error. Mine are only small (200ml liquid, 100ml vacuum space).

I'm not totally sure how a vacuum works. Presumably there is *some* oxygen in it or else the top of the jar would cave in? Just less than normal. And there is oxygen in the water.

Sorry to go a bit off topic. Nice write-up, Jeetered :thumbup:

:thumbup:

Edited by roquet (10/15/07 09:13 PM)

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nw_shroomy
NoN-stranger

Registered: 01/02/06
Posts: 1,332

Re: Jeetereds Easy Liquid Culture Tek

[Re: roquet]
#7520930 - 10/15/07 08:43 PM (16 years, 6 months ago)

Nice write up jeetered. :rockon:

:rockon:

Do you add all 60cc's of air all at once? After inoculating it or do you add a little at a time?

--------------------
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jeetered
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Re: Jeetereds Easy Liquid Culture Tek *DELETED*

[Re: nw_shroomy]
#7521071 - 10/15/07 09:15 PM (16 years, 6 months ago)

Post deleted by jeetered

Reason for deletion: 9o

Edited by jeetered (10/15/07 10:06 PM)

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nw_shroomy
NoN-stranger

Registered: 01/02/06
Posts: 1,332

Re: Jeetereds Easy Liquid Culture Tek

[Re: jeetered]
#7521117 - 10/15/07 09:30 PM (16 years, 6 months ago)

I didnt think I was picking it apart.If so sorry.I was just wondering when to shoot the air in.Thanks for the reply and the write up.

--------------------
Spawn Ratio Calculator
http://www.shroomery.org/forums/showflat.php/Number/7803673#7803673
I only grow edibles.Any info I give ONLY applies to gourmet mushrooms.

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jeetered
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Re: Jeetereds Easy Liquid Culture Tek

[Re: nw_shroomy]
#7521307 - 10/15/07 10:05 PM (16 years, 6 months ago)

Quote:

nw_shroomy said:
I didnt think I was picking it apart.If so sorry.I was just wondering when to shoot the air in.Thanks for the reply and the write up.

My apologies nw_shroomy, I didn't mean to come off abrasive please do not be afraid to ask any questions. I guess I was a little rude. SOmetimes it's hard to tell when someones genuinely asking questions, and being rhetorical.

Please, feel free to ask whatever you wish. For some reason I got the wrong impression about the intentions of your questions.

For that I apologize.

Peace bro! and to others please don't be afraid to ask anything. I honestly didnt mean to come off like that.

Edited by jeetered (10/15/07 10:07 PM)

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Skeeblix
Dave Thomas

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Re: Jeetereds Easy Liquid Culture Tek

[Re: jeetered]
#7521512 - 10/15/07 10:40 PM (16 years, 6 months ago)

Good stuff as ever, buddy.

--------------------
This post approved by:

Premedman1 said:
:lol:

:lol:

I just shat my pants.

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Blutjager
Inhuman

Registered: 06/11/06
Posts: 9,220

Re: Jeetereds Easy Liquid Culture Tek

[Re: Skeeblix]
#7521559 - 10/15/07 10:50 PM (16 years, 6 months ago)

I like

:hehehe:

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jeetered
Stranger

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Re: Jeetereds Easy Liquid Culture Tek

[Re: Blutjager]
#7521670 - 10/15/07 11:27 PM (16 years, 6 months ago)

Thanks skeeblix and blutjager!

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blood4blood
Calmer Than You Are

Registered: 04/25/07
Posts: 6,029
Loc: The Valley

Re: Jeetereds Easy Liquid Culture Tek

[Re: jeetered]
#7522378 - 10/16/07 09:20 AM (16 years, 6 months ago)

Quote:

jeetered said:
Quote:

blood4blood said:
nice one jeetered. how many cc's of spore solution did you add to the medium?

less then .5 cc's of spore solution.

so you added less than half a cc and got results like that. thats badass. i was always told to use between 2-3 cc's but if i can get by with only half a cc thats awesome. like i said before great write up man very easy to follow

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jeetered
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Re: Jeetereds Easy Liquid Culture Tek

[Re: blood4blood]
#7522615 - 10/16/07 10:52 AM (16 years, 6 months ago)

GTHANKS

and yea, you only need about a half cc of spore solution to inoculate the LC.

LC 2 LC transfers about 2 cc's for explosive growth.

Clone flesh takes about 4 days.

agar wedges of isolate rhizo takes about 3 days.

Pans take about 2 days.

lolz.

peace

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knotty
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Re: Jeetereds Easy Liquid Culture Tek

[Re: jeetered]
#7576512 - 10/30/07 12:37 PM (16 years, 5 months ago)

sweet tek, ive tried lc a couple times, i dont have a pc YET so i tried to steam sterilze it both times. didnt work. yo jeetered..have an extra pc laying around anywhere :bigblunt:

:bigblunt:

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Leonidas25
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Re: Jeetereds Easy Liquid Culture Tek

[Re: roquet]
#7579975 - 10/31/07 09:50 AM (16 years, 5 months ago)

OK, guys tell me if this would work. Instead of using a syringe with polyfill for the air exchange(s). Can't I just open a new sterile syringe/needle and suck up some air from the laminar flow coming out of my flow hood and inject that. That way I can carefully recap the syringe with a new un-opened sterile needle tip, and use the same syringe in the same manner at a later date for other air exchanges?

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jeetered
Stranger

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Re: Jeetereds Easy Liquid Culture Tek

[Re: Leonidas25]
#7580119 - 10/31/07 10:36 AM (16 years, 5 months ago)

Quote:

Leonidas25 said:
OK, guys tell me if this would work. Instead of using a syringe with polyfill for the air exchange(s). Can't I just open a new sterile syringe/needle and suck up some air from the laminar flow coming out of my flow hood and inject that. That way I can carefully recap the syringe with a new un-opened sterile needle tip, and use the same syringe in the same manner at a later date for other air exchanges?

that would work just fine.

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Leonidas25
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Re: Jeetereds Easy Liquid Culture Tek

[Re: jeetered]
#7589956 - 11/03/07 12:32 AM (16 years, 5 months ago)

I absolutely cannont find malt syrup anywhere locally...I already have the karo and everything else needed. Do you think 2 tbs of karo, in place of 1 tbs karo and 1 tbs malt, will be adequate for a cubensis strain?

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jeetered
Stranger

Registered: 07/07/06
Posts: 3,055
Loc: no clue

Re: Jeetereds Easy Liquid Culture Tek

[Re: Leonidas25]
#7590288 - 11/03/07 06:39 AM (16 years, 5 months ago)

that would work just fine.

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Leonidas25
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Re: Jeetereds Easy Liquid Culture Tek

[Re: jeetered]
#7590311 - 11/03/07 07:14 AM (16 years, 5 months ago)

Thanks Jeetered...this tek is awesome, really simple

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jeetered
Stranger

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Re: Jeetereds Easy Liquid Culture Tek

[Re: Leonidas25]
#7590369 - 11/03/07 08:01 AM (16 years, 5 months ago)

you're welcome and thanks!

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QDP843
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Re: Jeetereds Easy Liquid Culture Tek

[Re: jeetered]
#7641809 - 11/15/07 08:56 PM (16 years, 5 months ago)

thanks man, i will use this one day.

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Deception
HopefullyTripping Soon

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Re: Jeetereds Easy Liquid Culture Tek

[Re: QDP843]
#7679188 - 11/25/07 06:12 PM (16 years, 4 months ago)

This is awesome timing man!! :smile:

:smile:

i've been wanting to start an lc for quite awhile because for some reason my spore syringes keep failing to get the job done.. i've seen no results on 4 innoc. in a row so i want to turn my spore print into a lc. this tek is the easiest one i've read yet and i have everything i need to do it. thanks for the newb friendly post any all the info

--------------------
If at first you don't succeed..
Hit it till your knuckles bleed..

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jeetered
Stranger

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Re: Jeetereds Easy Liquid Culture Tek

[Re: Deception]
#7679256 - 11/25/07 06:34 PM (16 years, 4 months ago)

no problem!

good luck in your endeavors!

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